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  4. Human procollagen type III,HPCⅢ ELISA kit

Human procollagen type III,HPCⅢ ELISA kit

  • 中文名稱：
    人Ⅲ型前膠原（HPCⅢ）酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E11989h
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：

    This Human HPCⅢ ELISA Kit was designed for the quantitative measurement of Human HPCⅢ protein in serum. It is a Sandwich ELISA kit, its detection range is 25 ng/mL-300 ng/mL and the sensitivity is 25 ng/mL.

  • 別名：
    procollagen type 3 ,HPC3|procollagen type III ,HPCⅢ procollagen type III ,HPCIII
  • 縮寫：
    HPCⅢ
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum
  • 檢測范圍：
    25 ng/mL-300 ng/mL
  • 靈敏度：
    25 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<20%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human HPCⅢ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 84  
    Range % 80-92  
    1:2 Average % 97  
    Range % 91-105  
    1:4 Average % 93  
    Range % 90-96  
    1:8 Average % 95  
    Range % 89-99  
  • 回收率：
    The recovery of human HPCⅢ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 96 89-101  
    EDTA plasma (n=4) 97 92-102  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    300 1.356 1.342 1.349    
    200 0.967 1.023 0.995    
    100 0.466 0.482 0.474    
    50 0.211 0.199 0.205    
    25 0.050 0.048 0.049    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產品評價