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  4. Rat procollagen type III (HPCⅢ) ELISA Kit

Rat procollagen type III (HPCⅢ) ELISA Kit

  • 中文名稱：
    大鼠Ⅲ型前膠原（HPCⅢ）酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E15810r
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：

    This Rat HPCⅢ ELISA Kit was designed for the quantitative measurement of Rat HPCⅢ protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.

  • 別名：
    procollagen type 3 ,HPC3|procollagen type III ,HPCⅢ procollagen type III ,HPCIII
  • 縮寫：
    HPCⅢ
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測(cè)范圍：
    0.156 ng/mL-10 ng/mL
  • 靈敏度：
    0.039 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat HPCⅢ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:5 Average % 84  
    Range % 80-88  
    1:10 Average % 97  
    Range % 91-102  
    1:20 Average % 97  
    Range % 92-104  
    1:40 Average % 93  
    Range % 90-96  
  • 回收率：
    The recovery of rat HPCⅢ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 90-98  
    EDTA plasma (n=4) 97 92-104  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    10 2.589 2.636 2.613 2.426  
    5 1.901 1.967 1.934 1.747  
    2.5 1.455 1.468 1.462 1.275  
    1.25 1.012 1.089 1.051 0.864  
    0.625 0.587 0.597 0.592 0.405  
    0.312 0.342 0.368 0.355 0.168  
    0.156 0.282 0.289 0.286 0.099  
    0 0.188 0.186 0.187 　  
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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