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  4. Mouse procollagen type III (HPCⅢ) ELISA Kit

Mouse procollagen type III (HPCⅢ) ELISA Kit

  • 中文名稱：
    小鼠Ⅲ型前膠原（HPCⅢ）酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E17125m
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：

    This Mouse HPCⅢ ELISA Kit was designed for the quantitative measurement of Mouse HPCⅢ protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

  • 別名：
    procollagen type 3 ,HPC3|procollagen type III ,HPCⅢ procollagen type III ,HPCIII
  • 縮寫：
    HPCⅢ
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測范圍：
    15.6 pg/mL-1000 pg/mL
  • 靈敏度：
    3.9 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse HPCⅢ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:100 Average % 97    
    Range % 92-101    
    1:200 Average % 95    
    Range % 90-100    
    1:400 Average % 91    
    Range % 85-95    
    1:800 Average % 102    
    Range % 98-107    
  • 回收率：
    The recovery of mouse HPCⅢ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 89 85-93    
    EDTA plasma (n=4) 84 80-88    
                   
                   
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    pg/ml OD1 OD2 Average Corrected    
    1000 2.492 2.343 2.418 2.316    
    500 1.825 1.716 1.771 1.669    
    250 0.947 1.004 0.976 0.874    
    125 0.571 0.582 0.577 0.475    
    62.5 0.359 0.339 0.349 0.247    
    31.2 0.246 0.237 0.242 0.140    
    15.6 0.164 0.171 0.168 0.066    
    0 0.098 0.105 0.102 　    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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