SEMA4D

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Activity: Measured by its binding ability in a functional ELISA. Immobilized human SEMA4D at 2 μg/ml can bind Pepinemab, the EC₅₀ is 4.035-5.743 ng/mL.

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Activity: Measured by its binding ability in a functional ELISA. Immobilized Pepinemab at 2 μg/ml can bind Mouse Sema4d, the EC₅₀ is 4.878-7.446 ng/ml.

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Activity: Measured by its binding ability in a functional ELISA. Immobilized human SEMA4D at 2 μg/ml can bind biotinylated Pepinemab, the EC₅₀ is 1.005-1.776 ng/mL.

Activity: Measured by its binding ability in a functional ELISA. Immobilized human SEMA4D at 5 μg/ml can bind human PLXNB1 (CSB-MP018222HU2k6), the EC₅₀ is 0.8179-1.357 μg/mL.

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Activity: Measured by its binding ability in a functional ELISA. Immobilized Rhesus macaque SEMA4D at 2 μg/mL can bind Anti-SEMA4D recombinant antibody (CSB-RA835707A1HU), the EC₅₀ is 1.943-2.724 ng/mL.

The Binding Activity of Mouse Sema4d with Anti-SEMA4D recombinant antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Anti-SEMA4D recombinant antibody at 2 μg/mL can bind Mouse Sema4d (CSB-MP020990MO), the EC₅₀ is 5.829-16.86 ng/mL.

The Binding Activity of Human SEMA4D with Anti-SEMA4D recombinant antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Human SEMA4D (CSB-MP835707HU) at 2 μg/mL can bind Anti- SEMA4D recombinant antibody, the EC₅₀ is 60.38-152.6 ng/mL.

Untransfected HEK293T cells (green line) and transfected Human SEMA4D HEK293T stable cells (red line) were stained with anti-SEMA4D antibody (2μg/1*106cells), washed and then followed by FITC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.

Untransfected HEK293T cells (green line) and transfected Human SEMA4D HEK293T stable cells (red line) were stained with anti-SEMA4D antibody (2μg/1*106 cells), washed and then followed by FITC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.

The Binding Activity of Mouse Sema4d with Anti-SEMA4D recombinant antibody.
Activity: Measured by its binding ability in a functional ELISA. Immobilized Mouse Sema4d (CSB-MP020990MO) at 2 μg/mL can bind Anti-SEMA4D recombinant antibody, the EC₅₀ is 98.14-174.8 ng/mL.

The Binding Activity of Human SEMA4D with Anti-SEMA4D recombinant antibody.
Activity: Measured by its binding ability in a functional ELISA. Immobilized Human SEMA4D (CSB-MP835707HU) at 2 μg/mL can bind Anti-SEMA4D recombinant antibody, the EC₅₀ is 1.082-4.805 ng/mL.