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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Botryotinia fuckeliana U3 small nucleolar RNA-associated protein 25 (UTP25), partial CSB-YP412474BVD
CSB-EP412474BVD
CSB-BP412474BVD
CSB-MP412474BVD
CSB-EP412474BVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Botryotinia fuckeliana ATP-dependent RNA helicase dhh1 (dhh1), partial CSB-YP412475BVD
CSB-EP412475BVD
CSB-BP412475BVD
CSB-MP412475BVD
CSB-EP412475BVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Botryotinia fuckeliana Protein rot1 (rot1), partial CSB-YP412476BVD1
CSB-EP412476BVD1
CSB-BP412476BVD1
CSB-MP412476BVD1
CSB-EP412476BVD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Botryotinia fuckeliana Mitochondrial zinc maintenance protein 1, mitochondrial (MZM1), partial CSB-YP412477BVD
CSB-EP412477BVD
CSB-BP412477BVD
CSB-MP412477BVD
CSB-EP412477BVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Botryotinia fuckeliana Autophagy-related protein 2 (atg2), partial CSB-YP412478BVD
CSB-EP412478BVD
CSB-BP412478BVD
CSB-MP412478BVD
CSB-EP412478BVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Botryotinia fuckeliana Assembly factor cbp4 (cbp4), partial CSB-YP412479BVD1
CSB-EP412479BVD1
CSB-BP412479BVD1
CSB-MP412479BVD1
CSB-EP412479BVD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Botryotinia fuckeliana Cell division cycle protein 123 (cdc123) CSB-YP412480BVD
CSB-EP412480BVD
CSB-BP412480BVD
CSB-MP412480BVD
CSB-EP412480BVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Botryotinia fuckeliana ATP-dependent RNA helicase ded1 (ded1), partial CSB-YP412481BVD
CSB-EP412481BVD
CSB-BP412481BVD
CSB-MP412481BVD
CSB-EP412481BVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Botryotinia fuckeliana Autophagy-related protein 17 (atg17) CSB-YP412482BVD
CSB-EP412482BVD
CSB-BP412482BVD
CSB-MP412482BVD
CSB-EP412482BVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Botryotinia fuckeliana 54S ribosomal protein L4, mitochondrial (mrpl4) CSB-YP412483BVD
CSB-EP412483BVD
CSB-BP412483BVD
CSB-MP412483BVD
CSB-EP412483BVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Janthinobacterium sp. Chromosomal replication initiator protein DnaA (dnaA) CSB-YP412484JAQ
CSB-EP412484JAQ
CSB-BP412484JAQ
CSB-MP412484JAQ
CSB-EP412484JAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Janthinobacterium sp. Homoserine O-acetyltransferase (metX) CSB-YP412485JAQ
CSB-EP412485JAQ
CSB-BP412485JAQ
CSB-MP412485JAQ
CSB-EP412485JAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Janthinobacterium sp. Glutamyl-tRNA (Gln) amidotransferase subunit A CSB-YP412486JAQ
CSB-EP412486JAQ
CSB-BP412486JAQ
CSB-MP412486JAQ
CSB-EP412486JAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Janthinobacterium sp. N-acetyl-gamma-glutamyl-phosphate reductase (argC) CSB-YP412487JAQ
CSB-EP412487JAQ
CSB-BP412487JAQ
CSB-MP412487JAQ
CSB-EP412487JAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Janthinobacterium sp. Homoserine kinase (thrB) CSB-YP412488JAQ
CSB-EP412488JAQ
CSB-BP412488JAQ
CSB-MP412488JAQ
CSB-EP412488JAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Janthinobacterium sp. tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP412489JAQ
CSB-EP412489JAQ
CSB-BP412489JAQ
CSB-MP412489JAQ
CSB-EP412489JAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Janthinobacterium sp. (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB CSB-YP412490JAQ
CSB-EP412490JAQ
CSB-BP412490JAQ
CSB-MP412490JAQ
CSB-EP412490JAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Janthinobacterium sp. 30S ribosomal protein S21 (rpsU) CSB-YP412491JAQ
CSB-EP412491JAQ
CSB-BP412491JAQ
CSB-MP412491JAQ
CSB-EP412491JAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Janthinobacterium sp. Ribosome maturation factor RimM (rimM) CSB-YP412492JAQ
CSB-EP412492JAQ
CSB-BP412492JAQ
CSB-MP412492JAQ
CSB-EP412492JAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Janthinobacterium sp. Deoxycytidine triphosphate deaminase (dcd) CSB-YP412493JAQ
CSB-EP412493JAQ
CSB-BP412493JAQ
CSB-MP412493JAQ
CSB-EP412493JAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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