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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Actinobacillus succinogenes LexA repressor (lexA) CSB-YP409532AUG
CSB-EP409532AUG
CSB-BP409532AUG
CSB-MP409532AUG
CSB-EP409532AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Chaperone protein TorD (torD) CSB-YP409533AUG
CSB-EP409533AUG
CSB-BP409533AUG
CSB-MP409533AUG
CSB-EP409533AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Gamma-glutamyl phosphate reductase (proA) CSB-YP409534AUG
CSB-EP409534AUG
CSB-BP409534AUG
CSB-MP409534AUG
CSB-EP409534AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Peptide chain release factor 3 (prfC), partial CSB-YP409535AUG
CSB-EP409535AUG
CSB-BP409535AUG
CSB-MP409535AUG
CSB-EP409535AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes UPF0133 protein Asuc_0997 (Asuc_0997) CSB-YP409536AUG
CSB-EP409536AUG
CSB-BP409536AUG
CSB-MP409536AUG
CSB-EP409536AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Glutamyl-tRNA reductase (hemA) CSB-YP409537AUG
CSB-EP409537AUG
CSB-BP409537AUG
CSB-MP409537AUG
CSB-EP409537AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Pyridoxamine kinase (pdxY) CSB-YP409538AUG
CSB-EP409538AUG
CSB-BP409538AUG
CSB-MP409538AUG
CSB-EP409538AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes NADPH-dependent 7-cyano-7-deazaguanine reductase (queF) CSB-YP409539AUG
CSB-EP409539AUG
CSB-BP409539AUG
CSB-MP409539AUG
CSB-EP409539AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Glucose-1-phosphate adenylyltransferase (glgC) CSB-YP409540AUG
CSB-EP409540AUG
CSB-BP409540AUG
CSB-MP409540AUG
CSB-EP409540AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (dapD) CSB-YP409541AUG
CSB-EP409541AUG
CSB-BP409541AUG
CSB-MP409541AUG
CSB-EP409541AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Class B acid phosphatase (aphA) CSB-YP409542AUG
CSB-EP409542AUG
CSB-BP409542AUG
CSB-MP409542AUG
CSB-EP409542AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Integration host factor subunit alpha (ihfA) CSB-YP409543AUG
CSB-EP409543AUG
CSB-BP409543AUG
CSB-MP409543AUG
CSB-EP409543AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes 50S ribosomal protein L35 (rpmI) CSB-YP409544AUG
CSB-EP409544AUG
CSB-BP409544AUG
CSB-MP409544AUG
CSB-EP409544AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes 30S ribosomal protein S9 (rpsI) CSB-YP409545AUG
CSB-EP409545AUG
CSB-BP409545AUG
CSB-MP409545AUG
CSB-EP409545AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Lysine--tRNA ligase (lysS), partial CSB-YP409546AUG
CSB-EP409546AUG
CSB-BP409546AUG
CSB-MP409546AUG
CSB-EP409546AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Trp operon repressor homolog (trpR) CSB-YP409547AUG
CSB-EP409547AUG
CSB-BP409547AUG
CSB-MP409547AUG
CSB-EP409547AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Molybdenum cofactor biosynthesis protein C (moaC) CSB-YP409548AUG
CSB-EP409548AUG
CSB-BP409548AUG
CSB-MP409548AUG
CSB-EP409548AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Electron transport complex protein RnfD (rnfD), partial CSB-YP409549AUG
CSB-EP409549AUG
CSB-BP409549AUG
CSB-MP409549AUG
CSB-EP409549AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase CSB-YP409550AUG
CSB-EP409550AUG
CSB-BP409550AUG
CSB-MP409550AUG
CSB-EP409550AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP409551AUG
CSB-EP409551AUG
CSB-BP409551AUG
CSB-MP409551AUG
CSB-EP409551AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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