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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Actinobacillus succinogenes 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD) CSB-YP407952AUG
CSB-EP407952AUG
CSB-BP407952AUG
CSB-MP407952AUG
CSB-EP407952AUG-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes 30S ribosomal protein S20 (rpsT) CSB-YP407953AUG
CSB-EP407953AUG
CSB-BP407953AUG
CSB-MP407953AUG
CSB-EP407953AUG-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinomonas sp. UPF0301 protein Mmwyl1_0539 (Mmwyl1_0539) CSB-YP407954MNJ
CSB-EP407954MNJ
CSB-BP407954MNJ
CSB-MP407954MNJ
CSB-EP407954MNJ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinomonas sp. Acetylglutamate kinase (argB) CSB-YP407955MNJ
CSB-EP407955MNJ
CSB-BP407955MNJ
CSB-MP407955MNJ
CSB-EP407955MNJ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinomonas sp. Sec-independent protein translocase protein TatA (tatA), partial CSB-YP407956MNJ
CSB-EP407956MNJ
CSB-BP407956MNJ
CSB-MP407956MNJ
CSB-EP407956MNJ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinomonas sp. Bifunctional protein GlmU (glmU) CSB-YP407957MNJ
CSB-EP407957MNJ
CSB-BP407957MNJ
CSB-MP407957MNJ
CSB-EP407957MNJ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinomonas sp. Urease accessory protein UreG (ureG) CSB-YP407958MNJ
CSB-EP407958MNJ
CSB-BP407958MNJ
CSB-MP407958MNJ
CSB-EP407958MNJ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinomonas sp. Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP407959MNJ
CSB-EP407959MNJ
CSB-BP407959MNJ
CSB-MP407959MNJ
CSB-EP407959MNJ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinomonas sp. 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP407960MNJ
CSB-EP407960MNJ
CSB-BP407960MNJ
CSB-MP407960MNJ
CSB-EP407960MNJ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinomonas sp. L-ectoine synthase (ectC) CSB-YP407961MNJ
CSB-EP407961MNJ
CSB-BP407961MNJ
CSB-MP407961MNJ
CSB-EP407961MNJ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinomonas sp. Ribonuclease HII (rnhB) CSB-YP407962MNJ
CSB-EP407962MNJ
CSB-BP407962MNJ
CSB-MP407962MNJ
CSB-EP407962MNJ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinomonas sp. Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP407963MNJ
CSB-EP407963MNJ
CSB-BP407963MNJ
CSB-MP407963MNJ
CSB-EP407963MNJ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinomonas sp. Deoxycytidine triphosphate deaminase (dcd) CSB-YP407964MNJ
CSB-EP407964MNJ
CSB-BP407964MNJ
CSB-MP407964MNJ
CSB-EP407964MNJ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinomonas sp. Soluble pyridine nucleotide transhydrogenase (sthA) CSB-YP407965MNJ
CSB-EP407965MNJ
CSB-BP407965MNJ
CSB-MP407965MNJ
CSB-EP407965MNJ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinomonas sp. Dihydroorotate dehydrogenase (quinone) CSB-YP407966MNJ
CSB-EP407966MNJ
CSB-BP407966MNJ
CSB-MP407966MNJ
CSB-EP407966MNJ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinomonas sp. ATP synthase subunit b 1 (atpF1), partial CSB-YP407967MNJ
CSB-EP407967MNJ
CSB-BP407967MNJ
CSB-MP407967MNJ
CSB-EP407967MNJ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinomonas sp. Arginine deiminase (arcA) CSB-YP407968MNJ
CSB-EP407968MNJ
CSB-BP407968MNJ
CSB-MP407968MNJ
CSB-EP407968MNJ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinomonas sp. Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (accD) CSB-YP407969MNJ
CSB-EP407969MNJ
CSB-BP407969MNJ
CSB-MP407969MNJ
CSB-EP407969MNJ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinomonas sp. 50S ribosomal protein L32 (rpmF) CSB-YP407970MNJ
CSB-EP407970MNJ
CSB-BP407970MNJ
CSB-MP407970MNJ
CSB-EP407970MNJ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinomonas sp. Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP407971MNJ
CSB-EP407971MNJ
CSB-BP407971MNJ
CSB-MP407971MNJ
CSB-EP407971MNJ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式，可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中，除了表達系統(tǒng)之外，還面臨著一系列令人困惑的選擇，如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時，你必須做出很多決定。如果你選擇的明智，你將獲得高質(zhì)量的重組蛋白，后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定，你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里，來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示，可以幫助你做出明智的決策。

第一條：重組蛋白的生產(chǎn)，在這篇文章中，我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條：如何選擇合適的表達載體?

表達載體是一種DNA分子，它攜帶特定的基因進入宿主細胞，并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達，經(jīng)過多年的發(fā)展，表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示，本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條：如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用，對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題，并提供一些解決方案。除了以上文章，我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://cstxtech.com/technology-91.html。此外，我們還為我們的客戶提供重組蛋白表達服務(wù)，您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問，涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等，您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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