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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Brucella ovis 50S ribosomal protein L16 (rplP) CSB-YP403670BPI
CSB-EP403670BPI
CSB-BP403670BPI
CSB-MP403670BPI
CSB-EP403670BPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis 50S ribosomal protein L3 (rplC) CSB-YP403671BPI
CSB-EP403671BPI
CSB-BP403671BPI
CSB-MP403671BPI
CSB-EP403671BPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP403672BPI
CSB-EP403672BPI
CSB-BP403672BPI
CSB-MP403672BPI
CSB-EP403672BPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis Urease accessory protein UreD 2 (ureD2) CSB-YP403673BPI
CSB-EP403673BPI
CSB-BP403673BPI
CSB-MP403673BPI
CSB-EP403673BPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis DNA ligase (ligA), partial CSB-YP403674BPI
CSB-EP403674BPI
CSB-BP403674BPI
CSB-MP403674BPI
CSB-EP403674BPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis Cell-division control histidine kinase pdhS (pdhS), partial CSB-YP403675BPI
CSB-EP403675BPI
CSB-BP403675BPI
CSB-MP403675BPI
CSB-EP403675BPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis Protein TolB (tolB) CSB-YP403676BPI
CSB-EP403676BPI
CSB-BP403676BPI
CSB-MP403676BPI
CSB-EP403676BPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis UPF0335 protein BOV_1691 (BOV_1691) CSB-YP403677BPI
CSB-EP403677BPI
CSB-BP403677BPI
CSB-MP403677BPI
CSB-EP403677BPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis Protease HtpX homolog (htpX), partial CSB-YP403678BPI1
CSB-EP403678BPI1
CSB-BP403678BPI1
CSB-MP403678BPI1
CSB-EP403678BPI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis Glutamate 5-kinase (proB) CSB-YP403679BPI
CSB-EP403679BPI
CSB-BP403679BPI
CSB-MP403679BPI
CSB-EP403679BPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP403680BPI
CSB-EP403680BPI
CSB-BP403680BPI
CSB-MP403680BPI
CSB-EP403680BPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis Ribosome maturation factor RimM (rimM) CSB-YP403681BPI
CSB-EP403681BPI
CSB-BP403681BPI
CSB-MP403681BPI
CSB-EP403681BPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis Protein translocase subunit SecA (secA), partial CSB-YP403682BPI
CSB-EP403682BPI
CSB-BP403682BPI
CSB-MP403682BPI
CSB-EP403682BPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis ATP-dependent protease ATPase subunit HslU (hslU) CSB-YP403683BPI
CSB-EP403683BPI
CSB-BP403683BPI
CSB-MP403683BPI
CSB-EP403683BPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB CSB-YP403684BPI
CSB-EP403684BPI
CSB-BP403684BPI
CSB-MP403684BPI
CSB-EP403684BPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis HTH-type quorum sensing-dependent transcriptional regulator vjbR (vjbR) CSB-YP403685BPI
CSB-EP403685BPI
CSB-BP403685BPI
CSB-MP403685BPI
CSB-EP403685BPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis ATP phosphoribosyltransferase regulatory subunit (hisZ) CSB-YP403686BPI
CSB-EP403686BPI
CSB-BP403686BPI
CSB-MP403686BPI
CSB-EP403686BPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis 10 kDa chaperonin (groS) CSB-YP403687BPI
CSB-EP403687BPI
CSB-BP403687BPI
CSB-MP403687BPI
CSB-EP403687BPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis Protein nrdI (nrdI) CSB-YP403688BPI
CSB-EP403688BPI
CSB-BP403688BPI
CSB-MP403688BPI
CSB-EP403688BPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium tuberculosis DNA replication and repair protein recF (recF) CSB-YP403689MON
CSB-EP403689MON
CSB-BP403689MON
CSB-MP403689MON
CSB-EP403689MON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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