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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Clostridium thermocellum DNA polymerase IV (dinB) CSB-YP384018DUT
CSB-EP384018DUT
CSB-BP384018DUT
CSB-MP384018DUT
CSB-EP384018DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Recombination protein RecR (recR) CSB-YP384019DUT
CSB-EP384019DUT
CSB-BP384019DUT
CSB-MP384019DUT
CSB-EP384019DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum 30S ribosomal protein S18 (rpsR) CSB-YP384020DUT
CSB-EP384020DUT
CSB-BP384020DUT
CSB-MP384020DUT
CSB-EP384020DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum UPF0316 protein Cthe_2213 (Cthe_2213), partial CSB-YP384021DUT1
CSB-EP384021DUT1
CSB-BP384021DUT1
CSB-MP384021DUT1
CSB-EP384021DUT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum V-type ATP synthase subunit E (atpE) CSB-YP384022DUT
CSB-EP384022DUT
CSB-BP384022DUT
CSB-MP384022DUT
CSB-EP384022DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum V-type ATP synthase beta chain (atpB) CSB-YP384023DUT
CSB-EP384023DUT
CSB-BP384023DUT
CSB-MP384023DUT
CSB-EP384023DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Putative membrane protein insertion efficiency factor (Cthe_2368) CSB-YP384024DUT
CSB-EP384024DUT
CSB-BP384024DUT
CSB-MP384024DUT
CSB-EP384024DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Serine--tRNA ligase (serS) CSB-YP384025DUT
CSB-EP384025DUT
CSB-BP384025DUT
CSB-MP384025DUT
CSB-EP384025DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Glutamyl-tRNA reductase (hemA) CSB-YP384026DUT
CSB-EP384026DUT
CSB-BP384026DUT
CSB-MP384026DUT
CSB-EP384026DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum 50S ribosomal protein L11 (rplK) CSB-YP384027DUT
CSB-EP384027DUT
CSB-BP384027DUT
CSB-MP384027DUT
CSB-EP384027DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum 30S ribosomal protein S7 (rpsG) CSB-YP384028DUT
CSB-EP384028DUT
CSB-BP384028DUT
CSB-MP384028DUT
CSB-EP384028DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum UvrABC system protein C (uvrC), partial CSB-YP384029DUT
CSB-EP384029DUT
CSB-BP384029DUT
CSB-MP384029DUT
CSB-EP384029DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum SsrA-binding protein (smpB) CSB-YP384030DUT
CSB-EP384030DUT
CSB-BP384030DUT
CSB-MP384030DUT
CSB-EP384030DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP384031DUT
CSB-EP384031DUT
CSB-BP384031DUT
CSB-MP384031DUT
CSB-EP384031DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum 50S ribosomal protein L4 (rplD) CSB-YP384032DUT
CSB-EP384032DUT
CSB-BP384032DUT
CSB-MP384032DUT
CSB-EP384032DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum 30S ribosomal protein S19 (rpsS) CSB-YP384033DUT
CSB-EP384033DUT
CSB-BP384033DUT
CSB-MP384033DUT
CSB-EP384033DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum 50S ribosomal protein L14 (rplN) CSB-YP384034DUT
CSB-EP384034DUT
CSB-BP384034DUT
CSB-MP384034DUT
CSB-EP384034DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum 30S ribosomal protein S8 (rpsH) CSB-YP384035DUT
CSB-EP384035DUT
CSB-BP384035DUT
CSB-MP384035DUT
CSB-EP384035DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum 50S ribosomal protein L15 (rplO) CSB-YP384036DUT
CSB-EP384036DUT
CSB-BP384036DUT
CSB-MP384036DUT
CSB-EP384036DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum 30S ribosomal protein S4 (rpsD) CSB-YP384037DUT
CSB-EP384037DUT
CSB-BP384037DUT
CSB-MP384037DUT
CSB-EP384037DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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