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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Escherichia coli Peptidoglycan synthase FtsI (ftsI), partial CSB-YP364923ENV
CSB-EP364923ENV
CSB-BP364923ENV
CSB-MP364923ENV
CSB-EP364923ENV-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli D-alanyl-D-alanine-carboxypeptidase/endopeptidase AmpH (ampH) CSB-YP364924ENV
CSB-EP364924ENV
CSB-BP364924ENV
CSB-MP364924ENV
CSB-EP364924ENV-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Phe operon leader peptide (pheL) CSB-YP364925ENV
CSB-EP364925ENV
CSB-BP364925ENV
CSB-MP364925ENV
CSB-EP364925ENV-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Phenylalanyl--tRNA ligase operon leader peptide (pheM) CSB-YP364926ENV
CSB-EP364926ENV
CSB-BP364926ENV
CSB-MP364926ENV
CSB-EP364926ENV-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phenylalanyl--tRNA ligase operon leader peptide (pheM) CSB-YP364927EOD
CSB-EP364927EOD
CSB-BP364927EOD
CSB-MP364927EOD
CSB-EP364927EOD-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phenylalanyl--tRNA ligase operon leader peptide (pheM) CSB-YP364928SZB
CSB-EP364928SZB
CSB-BP364928SZB
CSB-MP364928SZB
CSB-EP364928SZB-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leu operon leader peptide (leuL) CSB-YP364929EGX
CSB-EP364929EGX
CSB-BP364929EGX
CSB-MP364929EGX
CSB-EP364929EGX-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leu operon leader peptide (leuL) CSB-YP364930SZB
CSB-EP364930SZB
CSB-BP364930SZB
CSB-MP364930SZB
CSB-EP364930SZB-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant PyrBI operon leader peptide (pyrL) CSB-YP364931EGX
CSB-EP364931EGX
CSB-BP364931EGX
CSB-MP364931EGX
CSB-EP364931EGX-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Thr operon leader peptide (thrL) CSB-YP364932ENV
CSB-EP364932ENV
CSB-BP364932ENV
CSB-MP364932ENV
CSB-EP364932ENV-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thr operon leader peptide (thrL) CSB-YP364933SZB
CSB-EP364933SZB
CSB-BP364933SZB
CSB-MP364933SZB
CSB-EP364933SZB-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tryptophanase leader peptide (tnaL) CSB-YP364934EGX
CSB-EP364934EGX
CSB-BP364934EGX
CSB-MP364934EGX
CSB-EP364934EGX-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Trp operon leader peptide (trpL) CSB-YP364935ENV
CSB-EP364935ENV
CSB-BP364935ENV
CSB-MP364935ENV
CSB-EP364935ENV-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Trp operon leader peptide (trpL) CSB-YP364936EOD
CSB-EP364936EOD
CSB-BP364936EOD
CSB-MP364936EOD
CSB-EP364936EOD-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Leu/Ile/Val-binding protein (livJ) CSB-YP364937ENV
CSB-EP364937ENV
CSB-BP364937ENV
CSB-MP364937ENV
CSB-EP364937ENV-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leu/Ile/Val-binding protein (livJ) CSB-YP364938EOD
CSB-EP364938EOD
CSB-BP364938EOD
CSB-MP364938EOD
CSB-EP364938EOD-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Branched-chain amino acid transport system 2 carrier protein (brnQ), partial CSB-YP364939EOD
CSB-EP364939EOD
CSB-BP364939EOD
CSB-MP364939EOD
CSB-EP364939EOD-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acyl-CoA thioesterase I (tesA) CSB-YP364940EGX
CSB-EP364940EGX
CSB-BP364940EGX
CSB-MP364940EGX
CSB-EP364940EGX-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Murein hydrolase activator NlpD (nlpD) CSB-YP364941SZB
CSB-EP364941SZB
CSB-BP364941SZB
CSB-MP364941SZB
CSB-EP364941SZB-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized lipoprotein yajG (yajG) CSB-YP364942EGX
CSB-EP364942EGX
CSB-BP364942EGX
CSB-MP364942EGX
CSB-EP364942EGX-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式，可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中，除了表達(dá)系統(tǒng)之外，還面臨著一系列令人困惑的選擇，如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí)，你必須做出很多決定。如果你選擇的明智，你將獲得高質(zhì)量的重組蛋白，后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定，你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里，來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示，可以幫助你做出明智的決策。

第一條：重組蛋白的生產(chǎn)，在這篇文章中，我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條：如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子，它攜帶特定的基因進(jìn)入宿主細(xì)胞，并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá)，經(jīng)過(guò)多年的發(fā)展，表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示，本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條：如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用，對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題，并提供一些解決方案。除了以上文章，我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://cstxtech.com/technology-91.html。此外，我們還為我們的客戶提供重組蛋白表達(dá)服務(wù)，您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn)，涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等，您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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