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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus subtilis Sensor histidine kinase yccG (yccG), partial CSB-YP302552BRJ1
CSB-EP302552BRJ1
CSB-BP302552BRJ1
CSB-MP302552BRJ1
CSB-EP302552BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Stress response protein CsbD (csbD) CSB-YP302553BRJ
CSB-EP302553BRJ
CSB-BP302553BRJ
CSB-MP302553BRJ
CSB-EP302553BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis 50S ribosomal protein L13 (rplM) CSB-YP302554BRJ
CSB-EP302554BRJ
CSB-BP302554BRJ
CSB-MP302554BRJ
CSB-EP302554BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative zinc metalloprotease ywhC (ywhC), partial CSB-YP302555BRJ1
CSB-EP302555BRJ1
CSB-BP302555BRJ1
CSB-MP302555BRJ1
CSB-EP302555BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Phosphatase rapF inhibitor (phrF) CSB-YP302556BRJ
CSB-EP302556BRJ
CSB-BP302556BRJ
CSB-MP302556BRJ
CSB-EP302556BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Antilisterial bacteriocin subtilosin biosynthesis protein AlbG (albG), partial CSB-YP302557BRJ1
CSB-EP302557BRJ1
CSB-BP302557BRJ1
CSB-MP302557BRJ1
CSB-EP302557BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis PTS system fructose-specific EIIABC component (fruA), partial CSB-YP302558BRJ
CSB-EP302558BRJ
CSB-BP302558BRJ
CSB-MP302558BRJ
CSB-EP302558BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Malonyl CoA-acyl carrier protein transacylase (fabD) CSB-YP302559BRJ
CSB-EP302559BRJ
CSB-BP302559BRJ
CSB-MP302559BRJ
CSB-EP302559BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Urease subunit beta (ureB) CSB-YP302560BRJ
CSB-EP302560BRJ
CSB-BP302560BRJ
CSB-MP302560BRJ
CSB-EP302560BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative phosphinothricin acetyltransferase YwnH (ywnH) CSB-YP302561BRJ
CSB-EP302561BRJ
CSB-BP302561BRJ
CSB-MP302561BRJ
CSB-EP302561BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Probable polysaccharide biosynthesis protein epsC (epsC), partial CSB-YP302562BRJ1
CSB-EP302562BRJ1
CSB-BP302562BRJ1
CSB-MP302562BRJ1
CSB-EP302562BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative acetyltransferase epsM (epsM) CSB-YP302563BRJ
CSB-EP302563BRJ
CSB-BP302563BRJ
CSB-MP302563BRJ
CSB-EP302563BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Enoyl-[acyl-carrier-protein] reductase [NADPH] FabL (fabL) CSB-YP302564BRJ
CSB-EP302564BRJ
CSB-BP302564BRJ
CSB-MP302564BRJ
CSB-EP302564BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium acetobutylicum Phosphate acetyltransferase (pta) CSB-YP302565DUB
CSB-EP302565DUB
CSB-BP302565DUB
CSB-MP302565DUB
CSB-EP302565DUB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydia muridarum 30S ribosomal protein S2 (rpsB) CSB-YP302566CIU
CSB-EP302566CIU
CSB-BP302566CIU
CSB-MP302566CIU
CSB-EP302566CIU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Putative colanic acid polymerase (wcaD), partial CSB-YP302567ENV
CSB-EP302567ENV
CSB-BP302567ENV
CSB-MP302567ENV
CSB-EP302567ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Putative colanic acid biosynthesis glycosyltransferase wcaL (wcaL) CSB-YP302568ENV
CSB-EP302568ENV
CSB-BP302568ENV
CSB-MP302568ENV
CSB-EP302568ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus ducreyi Ferric uptake regulation protein (fur) CSB-YP302569HSY
CSB-EP302569HSY
CSB-BP302569HSY
CSB-MP302569HSY
CSB-EP302569HSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus influenzae Putative type I restriction enzyme specificity protein HI_0216 (HI_0216) CSB-YP302570HTA
CSB-EP302570HTA
CSB-BP302570HTA
CSB-MP302570HTA
CSB-EP302570HTA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus influenzae Virulence-associated protein D (vapD) CSB-YP302571HTA
CSB-EP302571HTA
CSB-BP302571HTA
CSB-MP302571HTA
CSB-EP302571HTA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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