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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Proteus mirabilis Formate--tetrahydrofolate ligase (fhs), partial CSB-YP459542EYZ
CSB-EP459542EYZ
CSB-BP459542EYZ
CSB-MP459542EYZ
CSB-EP459542EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis 3-deoxy-manno-octulosonate cytidylyltransferase (kdsB) CSB-YP459543EYZ
CSB-EP459543EYZ
CSB-BP459543EYZ
CSB-MP459543EYZ
CSB-EP459543EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Elongation factor P-like protein (PMI0835) CSB-YP459544EYZ
CSB-EP459544EYZ
CSB-BP459544EYZ
CSB-MP459544EYZ
CSB-EP459544EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Phosphate acyltransferase (plsX) CSB-YP459545EYZ
CSB-EP459545EYZ
CSB-BP459545EYZ
CSB-MP459545EYZ
CSB-EP459545EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP459546EYZ
CSB-EP459546EYZ
CSB-BP459546EYZ
CSB-MP459546EYZ
CSB-EP459546EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Aspartate--tRNA ligase (aspS), partial CSB-YP459547EYZ
CSB-EP459547EYZ
CSB-BP459547EYZ
CSB-MP459547EYZ
CSB-EP459547EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP459548EYZ
CSB-EP459548EYZ
CSB-BP459548EYZ
CSB-MP459548EYZ
CSB-EP459548EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Recombination protein RecR (recR) CSB-YP459549EYZ
CSB-EP459549EYZ
CSB-BP459549EYZ
CSB-MP459549EYZ
CSB-EP459549EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Ribonuclease H (rnhA) CSB-YP459550EYZ
CSB-EP459550EYZ
CSB-BP459550EYZ
CSB-MP459550EYZ
CSB-EP459550EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Putative Holliday junction resolvase (PMI0338) CSB-YP459551EYZ
CSB-EP459551EYZ
CSB-BP459551EYZ
CSB-MP459551EYZ
CSB-EP459551EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Glutamate 5-kinase (proB) CSB-YP459552EYZ
CSB-EP459552EYZ
CSB-BP459552EYZ
CSB-MP459552EYZ
CSB-EP459552EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB CSB-YP459553EYZ
CSB-EP459553EYZ
CSB-BP459553EYZ
CSB-MP459553EYZ
CSB-EP459553EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis 3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase (fabA) CSB-YP459554EYZ
CSB-EP459554EYZ
CSB-BP459554EYZ
CSB-MP459554EYZ
CSB-EP459554EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Peptidyl-tRNA hydrolase (pth) CSB-YP459555EYZ
CSB-EP459555EYZ
CSB-BP459555EYZ
CSB-MP459555EYZ
CSB-EP459555EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Spermidine export protein MdtJ (mdtJ), partial CSB-YP459556EYZ1
CSB-EP459556EYZ1
CSB-BP459556EYZ1
CSB-MP459556EYZ1
CSB-EP459556EYZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Glycerol-3-phosphate acyltransferase (plsY), partial CSB-YP459557EYZ1
CSB-EP459557EYZ1
CSB-BP459557EYZ1
CSB-MP459557EYZ1
CSB-EP459557EYZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Peptide chain release factor 3 (prfC), partial CSB-YP459558EYZ
CSB-EP459558EYZ
CSB-BP459558EYZ
CSB-MP459558EYZ
CSB-EP459558EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Tryptophan synthase alpha chain (trpA) CSB-YP459559EYZ
CSB-EP459559EYZ
CSB-BP459559EYZ
CSB-MP459559EYZ
CSB-EP459559EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Ribosomal RNA small subunit methyltransferase C (rsmC) CSB-YP459560EYZ
CSB-EP459560EYZ
CSB-BP459560EYZ
CSB-MP459560EYZ
CSB-EP459560EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis 3-dehydroquinate dehydratase (aroQ) CSB-YP459561EYZ
CSB-EP459561EYZ
CSB-BP459561EYZ
CSB-MP459561EYZ
CSB-EP459561EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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