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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Escherichia coli Ribosomal protein L11 methyltransferase (prmA) CSB-YP457802ENT
CSB-EP457802ENT
CSB-BP457802ENT
CSB-MP457802ENT
CSB-EP457802ENT-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Phycocyanobilin lyase CpcT (cpcT) CSB-YP457803FQA
CSB-EP457803FQA
CSB-BP457803FQA
CSB-MP457803FQA
CSB-EP457803FQA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Photosystem Q (B) protein 3, partial CSB-YP457804FQA1
CSB-EP457804FQA1
CSB-BP457804FQA1
CSB-MP457804FQA1
CSB-EP457804FQA1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Transcriptional repressor NrdR (nrdR) CSB-YP457805FQA
CSB-EP457805FQA
CSB-BP457805FQA
CSB-MP457805FQA
CSB-EP457805FQA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP457806FQA
CSB-EP457806FQA
CSB-BP457806FQA
CSB-MP457806FQA
CSB-EP457806FQA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 30S ribosomal protein S5 (rpsE) CSB-YP457807FQA
CSB-EP457807FQA
CSB-BP457807FQA
CSB-MP457807FQA
CSB-EP457807FQA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 50S ribosomal protein L5 (rplE) CSB-YP457808FQA
CSB-EP457808FQA
CSB-BP457808FQA
CSB-MP457808FQA
CSB-EP457808FQA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 50S ribosomal protein L3 (rplC) CSB-YP457809FQA
CSB-EP457809FQA
CSB-BP457809FQA
CSB-MP457809FQA
CSB-EP457809FQA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Catalase-peroxidase (katG), partial CSB-YP457810FQA
CSB-EP457810FQA
CSB-BP457810FQA
CSB-MP457810FQA
CSB-EP457810FQA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP457811FQA
CSB-EP457811FQA
CSB-BP457811FQA
CSB-MP457811FQA
CSB-EP457811FQA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Elongation factor P (efp) CSB-YP457812FQA
CSB-EP457812FQA
CSB-BP457812FQA
CSB-MP457812FQA
CSB-EP457812FQA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Trigger factor (tig) CSB-YP457813FQA
CSB-EP457813FQA
CSB-BP457813FQA
CSB-MP457813FQA
CSB-EP457813FQA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Enolase (eno) CSB-YP457814FQA
CSB-EP457814FQA
CSB-BP457814FQA
CSB-MP457814FQA
CSB-EP457814FQA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Urease subunit gamma (ureA) CSB-YP457815FQA
CSB-EP457815FQA
CSB-BP457815FQA
CSB-MP457815FQA
CSB-EP457815FQA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Photosystem Q (B) protein 1, partial CSB-YP457816FQA1
CSB-EP457816FQA1
CSB-BP457816FQA1
CSB-MP457816FQA1
CSB-EP457816FQA1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Photosystem II reaction center protein K (psbK) CSB-YP457817FQA
CSB-EP457817FQA
CSB-BP457817FQA
CSB-MP457817FQA
CSB-EP457817FQA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP457818FQA
CSB-EP457818FQA
CSB-BP457818FQA
CSB-MP457818FQA
CSB-EP457818FQA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Phosphopantetheine adenylyltransferase (coaD) CSB-YP457819FQA
CSB-EP457819FQA
CSB-BP457819FQA
CSB-MP457819FQA
CSB-EP457819FQA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. D-alanine--D-alanine ligase (ddl) CSB-YP457820FQA
CSB-EP457820FQA
CSB-BP457820FQA
CSB-MP457820FQA
CSB-EP457820FQA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Phosphoglucosamine mutase (glmM) CSB-YP457821FQA
CSB-EP457821FQA
CSB-BP457821FQA
CSB-MP457821FQA
CSB-EP457821FQA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式，可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中，除了表達系統(tǒng)之外，還面臨著一系列令人困惑的選擇，如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時，你必須做出很多決定。如果你選擇的明智，你將獲得高質(zhì)量的重組蛋白，后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定，你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里，來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示，可以幫助你做出明智的決策。

第一條：重組蛋白的生產(chǎn)，在這篇文章中，我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條：如何選擇合適的表達載體?

表達載體是一種DNA分子，它攜帶特定的基因進入宿主細胞，并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達，經(jīng)過多年的發(fā)展，表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示，本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條：如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用，對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題，并提供一些解決方案。除了以上文章，我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://cstxtech.com/technology-91.html。此外，我們還為我們的客戶提供重組蛋白表達服務(wù)，您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問，涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等，您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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