E. coli biotin ligase
(BirA) is highly specific in covalently attaching biotin to the 15 amino
acid AviTag peptide. This recombinant protein was biotinylated in vivo
by AviTag-BirA technology, which method is BriA catalyzes amide linkage
between the biotin and the specific lysine of the AviTag.
The tag type will be
determined during production process. If you have specified tag
type, please tell us and we will develop the specified tag
preferentially.
產(chǎn)品提供形式:
Lyophilized powder Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復(fù)溶:
We recommend that this vial be briefly centrifuged prior
to opening to bring the contents to the bottom. Please reconstitute
protein in deionized sterile water to a concentration of 0.1-1.0
mg/mL.We recommend to add 5-50% of glycerol (final concentration) and
aliquot for long-term storage at -20℃/-80℃. Our default final
concentration of glycerol is 50%. Customers could use it as reference.
儲存條件:
Store at -20°C/-80°C upon receipt, aliquoting is necessary for
mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期:
The shelf life is related to many factors, storage state,
buffer ingredients, storage temperature and the stability of the protein
itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The
shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期:
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our
proteins are default shipped with normal blue ice packs, if you
request to ship with dry ice, please communicate with us in advance
and extra fees will be charged.
注意事項(xiàng):
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet :
Please contact us to get it.
產(chǎn)品評價(jià)
靶點(diǎn)詳情
功能:
Involved in at least 2 independent signaling cascades, controlling cell fate via canonical Wnt signaling and cell polarity via a planar cell polarity (PCP) cascade. Acts synergistically with dal/dapple-like to activate Wnt signaling, stabilizing ctnnb1/beta-catenin and leading to dorsal axis formation. Also prevents degradation of ctnnb1/beta-catenin by displacing gsk3 from a complex with ARP/Axin-related protein. Has an additional role in anterior-posterior (A/P) axis formation, specifying different neuroectodermal cell fates along the A/P axis in a dose-dependent manner by activating several early patterning genes. In the PCP pathway, required at the cell membrane for PCP-mediated neural and mesodermal convergent extension during gastrulation and subsequent neural tube closure, acting to activate jnk. Also involved in blastopore closure and archenteron elongation during early, but not late, gastrulation. Associates with ephrin receptors and ligands and acts as part of a downstream PCP pathway to mediate ephrin-mediated cell repulsion via activation of rhoa. Required for efnb1/ephrin-B1-driven movement of non-retinal progenitor cells into the retina during eye field formation. Patterns the hindbrain. Required for ciliogenesis. Controls the docking of basal bodies to the apical plasma membrane; mediates the activation, but not localization of rhoa at the apical surface of ciliated cells during basal body docking. Furthermore, required for the association of basal bodies with membrane-bound vesicles and the vesicle-trafficking protein exoc4/sec8, and this association is in turn required for basal body docking. Once basal bodies are docked, required for the planar polarization of basal bodies that underlies ciliary beating and the directional fluid flow across ciliated epithelia.
基因功能參考文獻(xiàn):
CapZIP, which has been shown to regulate ciliogenesis, is an ERK7 substrate, and that Dishevelled, which has also been shown to regulate ciliogenesis, facilitates ERK7 phosphorylation of CapZIP through binding to both ERK7 and CapZIP. PMID: 25823377
Different Dvl proteins (Dvl1, Dvl2, Dvl3) and the composition of dishevelled-beta-arrestin protein complexes contribute to the specific activation of individual branches of Wnt signaling in Xenopus gastrulation. PMID: 25568338
these findings suggest that regulation of Dvl protein levels by NEDD4L is essential for convergent extension during early Xenopus embryogenesis. PMID: 24833518
findings reveal that, in response to Wnt signalling, Dishevelled inhibits CSL transcription factors to regulate Notch signalling and cell-fate decisions in vivo PMID: 23132247
Data show that maternal Dvl2 and 3 are required for convergence extension movements downstream of the dorsally localized signaling pathway activated by Xnr3, but not downstream of the pathway activated by activin. PMID: 21618643
The archenteron elongation is independent of Xdsh signaling, especially during the second half of gastrulation. PMID: 15548584
Subcellular localization is insufficient to account for the pathway specificity of Dishevelled in the canonical Wnt versus PCP signaling cascades. PMID: 15936275
both Lgl and Dsh are required for normal apical-basal polarity of Xenopus ectodermal cells PMID: 16251968
The direct interaction of Dvl2 with AP-2 is important for Frizzled internalization and Frizzled/planar cell polarity signaling. PMID: 17199046
Dishevelled controls apical docking and planar polarization of basal bodies in ciliated epithelial cells. PMID: 18552847
PTK7-mediated membrane localization of a dsh deletion mutant lacking PCP activity inhibits neural crest migration. PMID: 19004858
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亞細(xì)胞定位:
Cytoplasm. Cytoplasmic vesicle. Cell projection, cilium. Nucleus. Cell membrane; Peripheral membrane protein. Note=Phosphorylated and recruited from the cytoplasm to the cell membrane by frizzled proteins. Also relocated to the cell membrane by sdc4 and ephb1/ephrin-B1. Concentrated at cell membrane in both ciliated and non-ciliated cells. Enriched at the apical surface of ciliated cells. Localized to the cilium rootlet.
蛋白家族:
DSH family
組織特異性:
Expressed equally in both animal-vegetal and dorsal-ventral directions of the early blastula. Becomes enriched on the dorsal side of the embryo after cortical rotation. Expressed along the anterior margin of eye field of neurulae (stage 16 embryos) and in