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Recombinant Human 5'-nucleotidase (NT5E) (Active)

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  • 中文名稱：
    人NT5E重組蛋白
  • 貨號：
    CSB-MP723415HU
  • 規(guī)格：
    ￥1368
  • 促銷：
    現(xiàn)貨重組蛋白特價促銷
  • 圖片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
    • Activity
      Measured by its binding ability in a functional ELISA. Immobilized CD73 at 2 μg/ml can bind Anti- CD73 Rabbit Monoclonal Antibody(CSB-RA978310A0HU), the EC50 is 3.212-4.525 ng/ml. Biological Activity Assay
  • 其他：

產(chǎn)品詳情

  • 純度：
    Greater than 95% as determined by SDS-PAGE.
  • 內(nèi)毒素：
    Less than 1.0 EU/ug as determined by LAL method.
  • 生物活性：
    Measured by its binding ability in a functional ELISA. Immobilized CD73 at 2 μg/ml can bind Anti- CD73 Rabbit Monoclonal Antibody(CSB-RA978310A0HU), the EC50 is 3.212-4.525 ng/ml.
  • 基因名：
  • Uniprot No.：
  • 別名：
    (5'-NT)(Ecto-5'-nucleotidase)(CD73)(NT5)(NTE)
  • 分子結(jié)構(gòu)：
  • 種屬：
    Homo sapiens (Human)
  • 蛋白長度：
    Full Length of Mature Protein
  • 來源：
    Mammalian cell
  • 分子量：
    60.2 kDa
  • 表達區(qū)域：
    27-549aa
  • 氨基酸序列
    WELTILHTNDVHSRLEQTSEDSSKCVNASRCMGGVARLFTKVQQIRRAEPNVLLLDAGDQYQGTIWFTVYKGAEVAHFMNALRYDAMALGNHEFDNGVEGLIEPLLKEAKFPILSANIKAKGPLASQISGLYLPYKVLPVGDEVVGIVGYTSKETPFLSNPGTNLVFEDEITALQPEVDKLKTLNVNKIIALGHSGFEMDKLIAQKVRGVDVVVGGHSNTFLYTGNPPSKEVPAGKYPFIVTSDDGRKVPVVQAYAFGKYLGYLKIEFDERGNVISSHGNPILLNSSIPEDPSIKADINKWRIKLDNYSTQELGKTIVYLDGSSQSCRFRECNMGNLICDAMINNNLRHTDEMFWNHVSMCILNGGGIRSPIDERNNGTITWENLAAVLPFGGTFDLVQLKGSTLKKAFEHSVHRYGQSTGEFLQVGGIHVVYDLSRKPGDRVVKLDVLCTKCRVPSYDPLKMDEVYKVILPNFLANGGDGFQMIKDELLRHDSGDQDINVVSTYISKMKVIYPAVEGRIKFS
  • 蛋白標簽：
    C-terminal 6xHis-tagged
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    3-7 business days
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 產(chǎn)品描述：

    The DNA fragment corresponding to Trp27-Ser549 of the human NT5E antigen is fused with a 6xHis-tag at the C-terminus and then is expressed in the mammalian cells. The resulting product is the recombinant human NT5E protein. Its purity is over 95% measured by SDS-PAGE. On the gel, this protein was migrated to the molecular weight band of about 62 kDa. The endotoxin content of this NT5E protein is less than 1.0 EU/ug determined by the LAL method. Its bioactivity has been validated through an ELISA. In the functional ELISA, immobilized NT5E can bind to anti-NT5E rabbit monoclonal antibody, with the EC50 of 3.212-4.525 ng/ml. This NT5E protein is available now.

    NT5E, also called CD73, is a bifunctional GPI-anchored cell surface protein with both enzymatic and nonenzymatic functions in cells. As an ecto-5′-nucleotidase, CD73 catalyzes the hydrolysis of AMP into adenosine and phosphate. As a membrane receptor for extracellular matrix protein (ECM), CD73 also modulates cell interaction with ECM components such as laminin and fibronectin, to mediate the invasion and metastasis of tumor cells. Overexpression of CD73 has been detected in many cancers, including gastric cancer, breast cancer, and colorectal cancer, and has been associated the poor clinical outcomes.

  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評價

平均分:
5.0分 - 2 個評價

實驗應用：細胞實驗

產(chǎn)品評價： 我使用CSB-MP723415HU產(chǎn)品進行ELISA實驗，EC50為 5.809-7.175ng/ml ，蛋白有較好的結(jié)合活性。蛋白性質(zhì)穩(wěn)定，重復性好，實驗進展順利，后續(xù)打算繼續(xù)大量采購。

By 曹老師

實驗應用：藥物相關研究

產(chǎn)品評價： CD73(NT5E)作為細胞外基質(zhì)蛋白(ECM)的膜受體，還可調(diào)節(jié)細胞與ECM成分（如層粘連蛋白和纖維連接蛋白）的相互作用，介導腫瘤細胞的侵襲和轉(zhuǎn)移。CD73在許多癌癥中都被檢測到過表達，包括胃癌、乳腺癌和結(jié)直腸癌，并與不良的臨床結(jié)果相關。我們使用CSB-MP723415HU做ELISA實驗，EC50為5.809-7.175ng/ml ，蛋白有較好的結(jié)合活性。HPLC驗證純度較高。后續(xù)用于抗體篩選，篩選的抗體親和力高，特異性好，靈敏度高。

By 彭老師

靶點詳情

  • 功能：
    Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Exhibits AMP-, NAD-, and NMN-nucleosidase activities.
  • 基因功能參考文獻：
    1. Changes in the local expression and activity of CD39 and CD73 in calcified valves suggest their potential role in calcific aortic valve disease. PMID: 30056298
    2. High expression of NT5E is associated with Prostate Cancer. PMID: 29916747
    3. Case Report: identify novel mutations in the NT5E gene in patient with calcification of joints and arteries. PMID: 28825389
    4. High CD73 expression is associated with breast cancer. PMID: 29047106
    5. High CD73 expression is associated with neoplasms. PMID: 29514610
    6. NT5E-targeting miRNAs (miR-30b and miR-340) function as tumor suppressors PMID: 29155108
    7. soluble CD73 might be used as serologic prognostic biomarker in patients with metastatic melanoma patients. PMID: 29202855
    8. High CD73 expression is associated with the increase in the ability of surviving breast cancer cells to evade innate and adaptive immunity. PMID: 29367423
    9. analysis of how inhibitors block human CD73 and block efficiently its enzymatic function in the low micromolar range through a non-competitive inhibition mechanism PMID: 29377887
    10. The Ecto-5'nucleotidase, together with alkaline phosphatase, also expressed apically in oviductal epithelium, complete the hydrolysis sequence by dephosphorylating AMP to adenosine. PMID: 29273916
    11. this paper shows that simultaneous overexpression of human E5NT and ENTPD1 protects porcine endothelial cells against H2O2-induced oxidative stress and cytotoxicity in vitro PMID: 28389406
    12. high levels of CD73 are significantly associated with reduced overall survival in patients with head and neck squamous cell carcinoma PMID: 27557512
    13. Studied role of miR-30a in colorectal cancer(CRC). Found miR-30a is downregulated in CRC, and it inhibits cell proliferation in CRC by reducing expression of CD73. PMID: 28464916
    14. we demonstrated the existence of CSCs in both cultured ccRCC cell line and tissues, and CD73 as a cell-surface biomarker for ccRCC CSC-like cells. PMID: 28404888
    15. Induction of ecto-5'-nucleotidase/CD73 by radiation contributes to the radiosensitivity of T24 urinary bladder cancer cell line. PMID: 29305710
    16. Report prognostic impact of CD73 expression in non-small lung cancers. PMID: 28060732
    17. Data show that MiR-422a and its target CD73 are involved in early loco(regional) recurrence of head and neck squamous cell carcinoma (HNSCC) tumors and are targets for personalized medicine. PMID: 27281619
    18. HPV+ cervical cancer cells express higher levels of CD73 than HPV- cells, and this expression is associated with the production of larger amounts of adenosine, as well as a stronger inhibition of proliferation, activation, and cytotoxic activity of CD8+ T cells via interaction with A2A adenosine receptor PMID: 28950987
    19. High CD73 expression is associated with Melanoma Metastasis. PMID: 28652244
    20. Hippocampal astrogliosis observed in medial temporal lobe epilepsy patients was accompanied by a proportionate increase in A2A receptor and ecto-5'-nucleotidase/CD73 immunoreactivities. PMID: 27650530
    21. High CD73 expression is associated with cervical cancer. PMID: 28202050
    22. Endogenous Plastic Somatic (ePS) cells in a latent state, i.e. lacking SOX2, OCT3/4 and NANOG (SON) expression, in non-diseased breast specimens through immunohistochemical analysis of previously identified ePS-specific biomarkers (CD73(+), EpCAM(+) and CD90(-)). PMID: 27705752
    23. Down-regulation of CD73 on B cells of patients with viremic HIV correlates with B cell activation and disease progression. PMID: 28193736
    24. CD73 polymorphisms play a role in calciphylaxis development in dialysis patients. PMID: 28212442
    25. CD73 expression is upregulated in NSCLC and is correlated with a decrease in miR-30a-5p expression. PMID: 28158983
    26. This study found that in septic critically ill patients the soluble CD73 levels were generally low and showed a further decrease from 0h to 24h. Moreover, the sCD73 levels were higher in acute kidney injury (AKI) versus non-AKI patients and in non-survivors with severe sepsis than in survivors, but were not independently associated either with the development of AKI or 90-day mortal PMID: 27732656
    27. concluded that CD39 and CD73 are molecular targets for the development of drugs for ALF patients care PMID: 27899277
    28. Genetic polymorphism of NT5E may contribute to the pathogenesis of calcific aortic valve disease. PMID: 27906615
    29. Oxidized low density lipoproteins modulate CD39 and CD73 activity in the endothelium. PMID: 27906627
    30. CD73 may play an important role in breast cancer stem cells. PMID: 27670764
    31. This study aimed to investigate the activities of purinergic system ecto-enzymes present on the platelet surface as well as CD39 and CD73 expressions on platelets of sickle cell anemia treated patients. PMID: 27044834
    32. Aim of the present study was to investigate the role of CD73 in glioma blood vessels; results found that the expression of CD73 in glioma vascular cells is significantly decreased, and this reduction may down-regulate the expression of brain microvascular endothelial tight junction-related proteins PMID: 26884147
    33. These results provide a finely mapped epitope that can be targeted for selective, potent, and non-competitive inhibition of CD73, as well as establish a strategy for inhibiting enzymes that function in both membrane-bound and soluble states. PMID: 26854859
    34. CD73 promotes the growth of human colorectal cancer cells through EGFR and the b-catenin/cyclin D1 signaling pathway. CD73 may be used as a valuable biomarker of colorectal cancer. PMID: 26708311
    35. Our study revealed that CD73 is an independent prognostic factor in prostate cancer. PMID: 26253870
    36. The NT5E gene is involved in both intrinsic and acquired resistance to platinum-based drugs in ovarian cancer. [meta-analysis] PMID: 26629888
    37. CD73 expression was significantly correlated with the invasion into adjacent organs. PMID: 26691441
    38. report on a Chinese family with CALJA and novel compound heterozygous mutations (c.1360G>A (p.Gly454Arg) and c.1387C>T (p.Arg463X)) in NT5E PMID: 26178434
    39. findings reveal that CD73-generated adenosine promotes epithelial integrity and suggest why loss of CD73 in endometrial cancer allows for tumor progression PMID: 26642367
    40. CD73 activity from any host cell type is not required for the monocyte/macrophage polarization in the peritoneum towards a pro- or an anti-inflammatory phenotype in vivo PMID: 26258883
    41. these data suggest a possible role for CD73 and A2A in inflammation observed in patients with T2D and obesity mediated via apoptosis. PMID: 25770019
    42. This study highlights a role for CD73 as a prognostic marker of patient survival and also as a candidate therapeutic target in high-grade serous ovarian cancers. PMID: 26363007
    43. role of CD73 and CD39 ectonucleotidases in T cell differentiation PMID: 26226423
    44. Expression of NPP1 and 5'-nucleotidase by valve interstitial cells promotes the mineralization of the aortic valve through A2aR and a cAMP/PKA/CREB pathway. PMID: 25644539
    45. Although upregulated CD73 expression in tumor cells correlates with a poor prognosis in patients with rectal adenocarcinoma, the combination of CD73 expression in malignant epithelial cells and tumor stroma may have a better prognostic value PMID: 25677906
    46. rs9444348 heterozygosity is associated with increased risk of post-traumatic epilepsy development after brain injury. PMID: 26040919
    47. Species-specific CD73 regulation, with potential significance to cancer, fibrosis, and other diseases characterized by changes in CD73 expression and function. PMID: 25298403
    48. The Nt5e(-/-) targeted mutant mice recapitulate some, but not all, features of ACDC and serve as a model system to study pharmacologic interventions for ectopic mineralization. PMID: 25486201
    49. CD73 enhances endothelial barrier function and sprouting in blood but not lymphatic vasculature. PMID: 25402681
    50. It decompose ATP and produce adenosine, which regulates immunity via adenosine receptor. PMID: 25675814

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  • 相關疾?。?/div>
    Calcification of joints and arteries (CALJA)
  • 亞細胞定位：
    Cell membrane; Lipid-anchor, GPI-anchor.
  • 蛋白家族：
    5'-nucleotidase family
  • 數(shù)據(jù)庫鏈接：

    HGNC: 8021

    OMIM: 129190

    KEGG: hsa:4907

    STRING: 9606.ENSP00000257770

    UniGene: Hs.153952