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  4. Recombinant Human Nuclear transcription factor Y subunit alpha (NFYA)

Recombinant Human Nuclear transcription factor Y subunit alpha (NFYA)

  • 中文名稱：
    人NFYA重組蛋白
  • 貨號：
    CSB-YP015773HU
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    人NFYA重組蛋白
  • 貨號：
    CSB-EP015773HU
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    人NFYA重組蛋白
  • 貨號：
    CSB-EP015773HU-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    人NFYA重組蛋白
  • 貨號：
    CSB-BP015773HU
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    人NFYA重組蛋白
  • 貨號：
    CSB-MP015773HU
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    CAAT box DNA binding protein subunit A ; CAAT box DNA-binding protein subunit A; CBF A; CBF B; CBFA; CBFB; CCAAT binding transcription factor subunit B; FLJ11236; HAP 2; HAP2; HAP2 CCAAT binding protein; NF YA; NF-YA; NFY protein chain A ; NFYA; NFYA_HUMAN; NUCLEAR FACTOR BINDING TO Y BOX OF HLA GENES; Nuclear transcription factor Y alpha; Nuclear transcription factor Y; Nuclear transcription factor Y subunit A; Nuclear transcription factor Y subunit alpha; Sez10; Transcription factor NF Y A subunit
  • 種屬：
    Homo sapiens (Human)
  • 蛋白長度：
    Full length protein
  • 表達區(qū)域：
    1-347
  • 氨基酸序列
    MEQYTANSNS STEQIVVQAG QIQQQQQGGV TAVQLQTEAQ VASASGQQVQ TLQVVQGQPL MVQVSGGQLI TSTGQPIMVQ AVPGGQGQTI MQVPVSGTQG LQQIQLVPPG QIQIQGGQAV QVQGQQGQTQ QIIIQQPQTA VTAGQTQTQQ QIAVQGQQVA QTAEGQTIVY QPVNADGTIL QQVTVPVSGM ITIPAASLAG AQIVQTGANT NTTSSGQGTV TVTLPVAGNV VNSGGMVMMV PGAGSVPAIQ RIPLPGAEML EEEPLYVNAK QYHRILKRRQ ARAKLEAEGK IPKERRKYLH ESRHRHAMAR KRGEGGRFFS PKEKDSPHMQ DPNQADEEAM TQIIRVS
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Component of the sequence-specific heterotrimeric transcription factor (NF-Y) which specifically recognizes a 5'-CCAAT-3' box motif found in the promoters of its target genes. NF-Y can function as both an activator and a repressor, depending on its interacting cofactors. NF-YA positively regulates the transcription of the core clock component ARNTL/BMAL1.
  • 基因功能參考文獻：
    1. Results from a study on gene expression variability markers in early-stage human embryos shows that NFYA is a putative expression variability marker for the 3-day, 8-cell embryo stage. PMID: 26288249
    2. Our results identified for the first time one of the mechanisms responsible for NF-Y expression, which may be involved in the aberrant expression and activity observed in tumor cells and other pathological conditions. PMID: 29505822
    3. Suggest NF-YAs and lamin A expression levels as novel potential biomarkers useful to identify G1 endometrial carcinoma patients with risk of recurrence. PMID: 27974701
    4. increased expression of NF-YA may promote a malignant phenotype in OS cells via modulation of FASN expression. PMID: 27840951
    5. The findings indicate that overexpression of NF-YA contributes to tumor angiogenesis through EZH2-STAT3 signaling in human melanoma cells, highlighting NF-YA as a potential therapeutic target in human melanoma. PMID: 27109360
    6. Data indicate that specific cancer-driving nodes are generally under NF-YA/B control. PMID: 26646448
    7. Presence of NF-Y transcription factor plays a pivotal role in transcriptional regulation of ID genes in development. PMID: 26509926
    8. our results indicate that NF-YA alternative splicing is an influential muscle cell determinant, through direct regulation of selected cell cycle blocking genes, and, directly and indirectly, of muscle-specific transcription factors PMID: 26921500
    9. A direct non-transcriptional role of NF-Y in the overall efficiency of DNA replication, specifically in the DNA elongation process. PMID: 26732297
    10. enhanced CDCA8 promoter activities by NF-Y overexpression and reduced CDCA8 transcription by NF-Y knockdown further verified that NF-Y is a positive regulator of CDCA8 transcription. PMID: 26170459
    11. Data show that Zinc-fingers and homeoboxes 2 (ZHX2) represses nuclear transcription factor Y alpha (NF-Y)-mediated activation of multidrug resistance 1 (MDR1) transcription. PMID: 25473899
    12. NF-Y inhibits NT2/D1 cell growth in p53-independent manner and decreases SOX2 expression. PMID: 25756534
    13. Stimulation of the lymphocytes with phytohemagglutinin, restores normal OGG1 levels and repair rates. MAP kinase c-Jun N-terminal kinase (JNK) and the recruitment of the transcription factor NFYA to the promoter region of OGG1 are shown to be involved. PMID: 25463392
    14. Association of p21 with NF-YA suppresses the expression of PLK1 and prevents mitotic death in response to DNA damage. PMID: 24407240
    15. NF-YA binds directly over the CCAAT sequence. PMID: 23595228
    16. NF-Y recruits the developmentally regulated, erythroid transcription activator GATA-2 and general repressor BCL11A to modulate transcription of the gamma-globin gene. PMID: 23071749
    17. The crystal structure of NF-Y bound to a 25 bp CCAAT oligonucleotide shows that the histone-fold domains dimer binds to the DNA sugar-phosphate backbone, mimicking the nucleosome H2A/H2B-DNA assembly; NF-YA both binds to NF-YB/NF-YC and inserts an alpha helix deeply into the DNA minor groove, providing sequence-specific contacts to the CCAAT box. PMID: 23332751
    18. NFYA could be a target of mutant TBP in SCA17. PMID: 22530004
    19. This study unveils a new role for NF-Y in the regulation of human proteasome genes. PMID: 22285817
    20. Sp1, CREB, HNF-1, and NF-Y, known to be responsive to hormones and diet, regulate NAGS transcription PMID: 22383952
    21. Findings establish that NF-Y acts upstream of E2F1 in p53-mediated apoptosis. PMID: 20952509
    22. A novel mechanism involving coordinated regulation of nuclear levels and acetylation of NF-YA and Bcl6 activates RGS4 transcription. PMID: 20630860
    23. The phospho-DeltaNp63alpha forms protein-protein complexes with NF-YA transcription factor regulate the transcription of DDIT3 gene implicated in the programmed cell death of head and neck squamous cell carcinoma cells upon cisplatin exposure. PMID: 20023394
    24. the immediate-early ICP0 gene may be among the first genes to be induced during the early events in HSV-1 reactivation, and NF-Y is important for this induction, and other factors induce the ICP22 promoter. PMID: 19828605
    25. immunodepletion of Oct-1 and NF-YA proteins or mutations in the OCT-1 and CAAT motifs disrupt BRCA1 binding to the GADD45 promoter PMID: 11777930
    26. NF-YA increases during CaCo-2 differentiation and that the constitutive expression of NF-YA, obtained in stably transfected CaCo-2 cells, results in the expression of differentiation markers PMID: 12392713
    27. confirms the important transactivating role of NF-YA isoforms for the cystathionine beta synthase 1b promoter via its synergism with Sp1 PMID: 12427542
    28. p300 binds to multiple NF-Y trimers to regulate cyclin B2 promoter function PMID: 12482752
    29. role in inhibiting von Willebrand factor promoter activation through recruitment of histone deacetylases PMID: 12511565
    30. NF-Y is a developmentally regulated inducer of the HOXB4 gene in hematopoietic cells. PMID: 12791656
    31. NF-YA is phosphorylated in a pathway involving Cdk2 PMID: 12857729
    32. Histone deacetylase inhibitors can induce Gadd45 through its promoter without the need for functional p53, and both Oct-1 and NF-Y concertedly participate in trichostatin A-induced activation of the gadd45 promoter. PMID: 14586402
    33. NF-YA has a role in regulating the induction of human OGG1 following administration of DNA-alkylating agents PMID: 14688259
    34. p53 negatively regulates Chk2 gene transcription through modulation of NF-Y function and that this regulation may be important for reentry of cells into the cell cycle after DNA damage is repaired. PMID: 15044452
    35. NF-Y has a role in regulating EPHX1 expression through its interaction with C/EBPalpha PMID: 15150264
    36. transforming growth factor beta type II receptor promoter activity and acetylation of Sp1 by recruitment of PCAF/p300 to a Sp1.NF-Y complex are induced by Trichostatin A PMID: 15647279
    37. These results indicate that 1,25(OH)2D3 may affect transcription of the human PTH gene both by competitive binding of VDR and NF-Y, and by modulating transcriptional activity of NF-Y. PMID: 15707954
    38. NF-Ya is a potent cellular regulator of hematopoietic stem cell self-renewal PMID: 16081537
    39. Data suggest that the cell specifity of HIV-1 expression and replication may be regulated, in part, through the control of TRBP1 expression by NF-Y factors. PMID: 16343534
    40. cyclin B2 expression in colorectal adenocarcinoma is dependent on NF-Y PMID: 17289878
    41. Our data indicate that multiple CCAAT control elements are involved in the regulation of the SOX3 promoter, suggesting that NF-Y functions as a key regulator of SOX3 gene expression. PMID: 17910945
    42. NF-Y is an important mediator of EBV stress response in switching from a latent to lytic state PMID: 18281530
    43. p53 overexpression strongly downregulates the transcriptional efficiency driven by an H ferritin promoter construct containing only the NF-Y recognition sequence. PMID: 18372207
    44. NF-Y is a fundamental switch at the heart of decision between gene activation and repression in CCAAT regulated genes. PMID: 18446193
    45. Transcription factors Sp3, ZBP-89 and NF-Y are capable of binding to the SOX18 promoter region. PMID: 18496767
    46. The existence of a functional binding site for the tumor suppressor p53 near the proximal CCAAT box and the fact that the basal expression of annexin A1 in human colon adenocarcinoma cells is driven by p53 at the transcriptional level, is shown. PMID: 18541673
    47. Genome-wide occupancy of SREBP1 and its partners NFY and SP1 reveals novel functional roles and combinatorial regulation of distinct classes of genes. PMID: 18654640
    48. O-GlcNAc of Sp1 serine/threonine-rich region interrupts a physical interaction between Sp1 and NF-YA, thus inhibiting Sp1-NF-Y cooperative activation of gene transcription. PMID: 19302979
    49. review: role of the C/EBPbeta isoforms in breast cancer PMID: 19351437
    50. NF-Y (complex of NFYA, NFYB, and NFYC) was initially described as the factor binding to the Y box of MHC class II genes, an essential element of MHC class II gene enhancers and promoters. PMID: 3476205

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  • 亞細胞定位：
    Nucleus.
  • 蛋白家族：
    NFYA/HAP2 subunit family
  • 數據庫鏈接：

    HGNC: 7804

    OMIM: 189903

    KEGG: hsa:4800

    STRING: 9606.ENSP00000345702

    UniGene: Hs.10441