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Recombinant Human C-C chemokine receptor type 8 (CCR8)-VLPs (Active)

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  • 中文名稱：
  • 貨號(hào)：
    CSB-MP004847HU
  • 規(guī)格：
    ￥5000
  • 圖片：
    • CSB-MP004847HU is detected by Mouse anti-6*His monoclonal antibody.
    • CSB-MP004847HU is detected by Anti-CCR8 recombinant Antibody
    • Activity
      Measured by its binding ability in a functional ELISA. Immobilized human CCR8 (CSB-MP004847HU) at 5 μg/ml can bind Anti-CCR8 recombinant Antibody, the the EC50 is 11.13-17.29 ng/ml.
    • The presence of VLP-like structures was confirmed by TEM
    • Loaded Anti-Human CCR8 Antibody (CSB-RA004847MA3HU) on 96-Flat plate, can bind Human CCR8, with an affinity constant of ＜1 pM as determined in BLI assay (Gator Prime).
    • The purity of VLPs was greater than 90% as determined by SEC-HPLC
  • 其他：

產(chǎn)品詳情

  • 純度：
    Greater than 90% as determined by SEC-HPLC.
  • 內(nèi)毒素：
    Less than 1.0 EU/ug as determined by LAL method.
  • 生物活性：
    Measured by its binding ability in a functional ELISA. Immobilized human CCR8 (CSB-MP004847HU) at 5 μg/ml can bind Anti-CCR8 recombinant Antibody, the the EC50 is 11.13-17.29 ng/ml.
  • 基因名：
  • Uniprot No.：
  • 別名：
    CCR8; CKRL1; CMKBR8; CMKBRL2; C-C chemokine receptor type 8; C-C CKR-8; CC-CKR-8; CCR-8; CC chemokine receptor CHEMR1; Chemokine receptor-like 1; CKR-L1; GPR-CY6; GPRCY6; TER1; CD antigen CDw198
  • 種屬：
    Homo sapiens (Human)
  • 蛋白長度：
    Full Length
  • 來源：
    Mammalian cell
  • 分子量：
    42.2 kDa
  • 表達(dá)區(qū)域：
    1-355aa
  • 氨基酸序列
    MDYTLDLSVTTVTDYYYPDIFSSPCDAELIQTNGKLLLAVFYCLLFVFSLLGNSLVILVLVVCKKLRSITDVYLLNLALSDLLFVFSFPFQTYYLLDQWVFGTVMCKVVSGFYYIGFYSSMFFITLMSVDRYLAVVHAVYALKVRTIRMGTTLCLAVWLTAIMATIPLLVFYQVASEDGVLQCYSFYNQQTLKWKIFTNFKMNILGLLIPFTIFMFCYIKILHQLKRCQNHNKTKAIRLVLIVVIASLLFWVPFNVVLFLTSLHSMHILDGCSISQQLTYATHVTEIISFTHCCVNPVIYAFVGEKFKKHLSEIFQKSCSQIFNYLGRQMPRESCEKSSSCQQHSSRSSSVDYIL
  • 蛋白標(biāo)簽：
    C-terminal 10xHis-tagged (This tag can be tested only under denaturing conditions)
  • 產(chǎn)品提供形式：
    Liquid
    Note: We will default ship it in lyophilized form with normal bule ice packs. However, if you request to ship in liquid form, it needs to be shipped with dry ice, please communicate with us in advance and extra fees for dry ice and dry ice box will be charged.
  • 緩沖液：
    PBS, pH 7.4, 6% trehalose
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 產(chǎn)品描述：

    Recombinant full-length human CCR8 protein was expressed by the intact DNA sequence of the human CCR8 in mammalian cells. This recombinant CCR8 protein was fused with a 10xHis-tag at the C-terminus. It contains less than 1.0 EU/ug determined by the LAL method. The CCR8 protein is biologically active as it can react with the mouse anti-6xHis monoclonal antibody and anti-CCR8 recombinant antibody, respectively. Its VLP-like structure was observed and confirmed by TEM. This recombinant CCR8 protein is highly immunogenic because it carries viral protein.

    Past studies have shown that the CCR8 protein is mainly expressed on monocytes, Th2 cells, and Treg cells. Many basic studies have also proved CCR8 plays an essential role in the biological processes of lymph node migration of monocyte-derived dendritic cells and Th2 cell-mediated type 2 inflammation. Researchers have found that CCR8 is a good biomarker of Treg cells at tumor sites and a potential tumor immune target. Subsequent studies found that the presence or absence of CCR8 does not affect the migration ability of Treg and its function in the tumor microenvironment. The development of CCR8-targeted monoclonal antibody drugs is expected to specifically inhibit the function of Treg at the tumor site and contribute to cancer treatment.

  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

平均分:
5.0分 - 1 個(gè)評(píng)價(jià)

實(shí)驗(yàn)應(yīng)用：藥物相關(guān)研究

產(chǎn)品評(píng)價(jià)： CCR8多次跨膜，僅表達(dá)其胞外結(jié)構(gòu)域會(huì)破壞其構(gòu)象表位，造成部分抗原表位的丟失。華美生物公司表達(dá)全長蛋白，且具有較好的生物活性和免疫原性，對(duì)于我們后續(xù)開發(fā)抗體藥物具有很大的幫助。贊贊贊

By 錢老師

問答及客戶評(píng)論

■ 常見問題解答
Q:

我注意到你提供了 CCR8 納米圓盤，你能告訴我細(xì)胞內(nèi)和細(xì)胞外結(jié)構(gòu)域的暴露情況嗎？ 我們想篩選一組能夠檢測(cè)該靶標(biāo)的細(xì)胞內(nèi)或細(xì)胞外表位的抗體； 對(duì)于 VLP，根據(jù)定義，細(xì)胞內(nèi)結(jié)構(gòu)域沒有暴露，并不適合我的實(shí)驗(yàn)。

A:
VLP 只暴露胞外域 ，胞內(nèi)域在包膜里面不暴露，主要就是篩識(shí)別胞外表位，所以VLP的可能不適合您的實(shí)驗(yàn)。 如果您想篩選一組能夠檢測(cè)該靶標(biāo)的細(xì)胞內(nèi)或細(xì)胞外表位的抗體，推薦：CSB-CF004847HU 是通過無細(xì)胞表達(dá)系統(tǒng)表達(dá)的蛋白全長，但是沒有測(cè)活，不能保證識(shí)別內(nèi)源樣本，可以小規(guī)格訂購嘗試。或者您可以選擇目的蛋白的胞內(nèi)域或胞外域序列進(jìn)行重組表達(dá)后，篩選識(shí)別靶標(biāo)的細(xì)胞內(nèi)或細(xì)胞外表位的抗體，但無法保證識(shí)別內(nèi)源性樣本。

靶點(diǎn)詳情

  • 功能：
    Receptor for the chemokine CCL1/SCYA1/I-309. May regulate monocyte chemotaxis and thymic cell line apoptosis. Alternative coreceptor with CD4 for HIV-1 infection.
  • 基因功能參考文獻(xiàn)：
    1. Authors demonstrate the pivotal role of CCR8(+) Treg cells in restraining immunity and highlight the potential clinical implications of this discovery. PMID: 28533380
    2. Role of Conserved Disulfide Bridges and Aromatic Residues in Extracellular Loop 2 of Chemokine Receptor CCR8 for Chemokine and Small Molecule Binding. PMID: 27226537
    3. High CCR8 expression is associated with high recurrence in kidney cancer. PMID: 26716905
    4. findings suggest that CCR8 expression in ALCL is more closely related to the presence of DUSP22 rearrangements than to cutaneous involvement and that the function of CCR8 may extend beyond its skin-homing properties in this disease PMID: 25390351
    5. Epidermal-derived vitamin D3 metabolites and prostaglandins provide an essential cue for the localization of CCR8+ immune surveillance T cells within healthy human skin. PMID: 26002980
    6. CCL1-CCR8 interaction may play a critical role in lymphocytic recruitment in IgG4-related sclerosing cholangitis and type 1 autoimmune pancreatitis, leading to duct-centred inflammation and obliterative phlebitis. PMID: 23811304
    7. Identification of human CCR8 as a CCL18 receptor. PMID: 23999500
    8. CCR8(+) myeloid cell subset is expanded in patients with cancer. PMID: 23363815
    9. Data show that CCR8 expression by newly activated naive T cells is regulated by skin-specific factor(s) derived primarily from epidermal keratinocytes. PMID: 23043070
    10. C-terminal clipping of chemokine CCL1/I-309 enhances CCR8-mediated intracellular calcium release and anti-apoptotic activity PMID: 22479563
    11. The functional data from human macrophages suggest a potential cross talk between the CCR8 and the Toll-like receptor 4 (TLR4) pathways, both of which are present in chronic obstructive pulmonary disease patients. PMID: 21976223
    12. There may be a role for CCR8 in the recruitment of T cells to the lung in asthmatics. PMID: 20455898
    13. CCR8 mediates rescue from dexamethasone-induced apoptosis via an ERK-dependent pathway PMID: 12525579
    14. found in the central nervous system and is associated with phagocytic macrophages PMID: 12547701
    15. CCR8 genes and surrounding genomic regions these genes are the result of the duplication of an ancestral gene prior to the divergence of teleost fish. PMID: 12551893
    16. Transfected human CCR8-dependent activation of the RAS/MAPK pathway mediates anti-apoptotic activity of I-309/ CCL1 and vMIP-I. PMID: 12645948
    17. The induction of CCR8 under conditions associated with vascular smooth muscle cell proliferation and migration raises the possibility that CCR8 may play an important role in vessel wall pathology. PMID: 14576057
    18. the axis CCL1-CCR8 links adaptive and innate immune functions that play a role in the initiation and amplification of atopic skin inflammation PMID: 15814739
    19. CCR8 ligands are allotropic, binding to distinct sites within CCR8; the human immune system may have evolved to use CCL7 as a selective antagonist of viral chemokine activity at CCR8 but not those of the host ligand PMID: 17023422
    20. CCR8 is expressed by a small and heterogeneous population of peripheral blood CD4 memory T cells enriched in T helper type 2 (Th2) effector and T regulatory (Treg) cells. PMID: 17082609
    21. CCR8-expressing CD4-positive T lymphocytes are preferentially recruited from the periphery into the lungs of asthmatic individuals, driven by elevated CCL1 levels produced almost exclusively by mast cells and basophils. PMID: 17641040
    22. The combination of 17beta-E(2) with the environmental pollutant TCDD is involved in the pathogenesis of endometriosis via up-regulating the chemokine CCR8-I-309. PMID: 17693327

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  • 亞細(xì)胞定位：
    Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    G-protein coupled receptor 1 family
  • 數(shù)據(jù)庫鏈接：

    HGNC: 1609

    OMIM: 601834

    KEGG: hsa:1237

    STRING: 9606.ENSP00000326432

    UniGene: Hs.113222