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  4. Rat amyloid beta peptide 1-42,Aβ1-42 ELISA Kit

Rat amyloid beta peptide 1-42,Aβ1-42 ELISA Kit

  • 中文名稱：
    大鼠β淀粉樣蛋白1-42(Aβ1-42)酶聯免疫試劑盒
  • 貨號：
    CSB-E10786r
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3900/￥2500
  • 其他：

產品詳情

  • 產品描述：

    This Rat Aβ1-42 ELISA Kit was designed for the quantitative measurement of Rat Aβ1-42 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.9 pg/mL-60 pg/mL and the sensitivity is 0.225 pg/mL .

  • 別名：
    Amyloid beta 42(ABeta 42),amyloid beta peptide 1-42,Aβ1-42,amyloid βpeptide 1-42
  • 縮寫：
    Aβ1-42
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    0.9 pg/mL-60 pg/mL
  • 靈敏度：
    0.225 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Neuroscience
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat Aβ1-42 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %87
    Range %83-90
    1:2Average %94
    Range %90-98
    1:4Average %101
    Range %97-104
    1:8Average %96
    Range %93-99
  • 回收率：
    The recovery of rat Aβ1-42 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9489-98
    EDTA plasma (n=4)10095-105
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    602.281 2.274 2.278 2.136
    301.599 1.576 1.588 1.446
    150.967 0.924 0.946 0.804
    7.50.538 0.528 0.533 0.391
    3.750.395 0.371 0.383 0.241
    1.80.321 0.312 0.317 0.175
    0.90.235 0.250 0.243 0.101
    00.141 0.143 0.142 　
  • 數據處理：
  • 貨期：
    3-5 working days

引用文獻

產品評價

問答及客戶評論

■ 常見問題解答
Q:

我們的實驗室打算購買β淀粉樣蛋白1-42，Aβ1-42 ELISA試劑盒，貨號是CSB-E10786r。但是，我們使用的是含有SDS的RIPA緩沖液來裂解組織，同時我們也用相同的RIPA緩沖液和1x PBS稀釋了樣品。我想知道這樣做是否會影響試劑盒的效果

A:
對于ELISA檢測，一般不建議使用含有SDS的緩沖液處理樣品。這是因為SDS可以破壞分子與分子間的氫鍵，即破壞蛋白質的二級和三級結構，并可能影響ELISA的檢測。由于您的樣品已經被處理過，我們只能建議您先使用處理后的樣品進行梯度預實驗。我們以前沒有這樣做過，所以我們只能建議你試一試。