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  4. Human amyloid beta peptide 1-42,Aβ1-42 ELISA Kit

Human amyloid beta peptide 1-42,Aβ1-42 ELISA Kit

  • 中文名稱：
    人β淀粉樣蛋白1-42(Aβ1-42)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E10684h
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
        Aβ1-42是beta淀粉樣蛋白（Aβ）的一種形式，它是一種由神經(jīng)元合成和分泌的蛋白質(zhì)。Aβ1-42是導致阿爾茨海默病（AD）的重要成分之一。它在AD病變過程中聚集成膠質(zhì)斑和神經(jīng)纖維纏結，損害神經(jīng)元的正常功能。此外，Aβ1-42還可能在其他神經(jīng)系統(tǒng)疾病的發(fā)病機制中發(fā)揮作用，包括創(chuàng)傷性腦損傷、脊髓灰質(zhì)炎和肌萎縮側索硬化癥等。
        華美生物所提供的Human amyloid beta peptide 1-42,Aβ1-42 ELISA Kit屬于ELISA檢測試劑盒，采用雙抗夾心法定量檢測人血清、血漿、組織勻漿、腦脊液(CSF)樣本中的Aβ1-42，其靈敏度為0.078 ng/mL，檢測范圍為0.312 ng/mL-20 ng/mL。
     
  • 別名：
    Amyloid beta 42(ABeta 42),amyloid beta peptide 1-42,Aβ1-42,amyloid βpeptide 1-42
  • 縮寫：
    Aβ1-42
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates, cerebrospinal fluid (CSF)
  • 檢測范圍：
    0.312 ng/mL-20 ng/mL
  • 靈敏度：
    0.078 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Neuroscience
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human Aβ1-42 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 84  
    Range % 80-92  
    1:2 Average % 97  
    Range % 91-105  
    1:4 Average % 97  
    Range % 92-110  
    1:8 Average % 93  
    Range % 86-98  
  • 回收率：
    The recovery of human Aβ1-42 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 89-98  
    EDTA plasma (n=4) 97 90-100  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    20 2.302 2.379 2.341 2.244  
    10 1.762 1.781 1.772 1.675  
    5 1.124 1.189 1.157 1.060  
    2.5 0.653 0.679 0.666 0.569  
    1.25 0.375 0.398 0.387 0.290  
    0.625 0.251 0.259 0.255 0.158  
    0.312 0.151 0.153 0.152 0.055  
    0 0.095 0.098 0.097 　  
  • 本試劑盒所含材料：
      • A micro ELISA plate --- The 96-well plate has been pre-coated with anti-human Aβ1-42 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
      • Two vials lyophilized standard --- Dilute a bottle of standard at dilution series, read the OD values, and then draw a standard curve.
      • One vial Biotin-labeled Aβ1-42 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
      • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
      • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the high concentration Biotin-antibody to an appropriate working solution.
      • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the high concentration HRP-avidin solution to an appropriate solution.
      • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
      • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) --- Wash away unbound or free substances.
      • One vial TMB Substrate (10 ml/bottle) --- Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
      • One vial Stop Solution (10 ml/bottle) --- Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
      • An instruction manual

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  • 本試劑盒不含材料：
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
      • An incubator can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻

產(chǎn)品評價

問答及客戶評論

■ 常見問題解答
Q:

We are interested in following item.
#CSB-E10684h
Do you have the information of capture antibody coated on the plate and biotinylated detection antibody in the kit?

A:
Thanks for your inquiry.Regarding the mentioned kit,we would like to inform the following information:
(Note:Capture:capture antibody coated on the plate;Detection: biotin-conjugated antibody)
#CSB-E10684h,Human amyloid beta peptide 1-42,Aβ1-42 ELISA Kit
Capture:mouse monoclonal antibody;Detection:goat polyclonal antibody
■ 客戶評論
  • Sample type: Brain tissues
    Sample species: Human
    Review: the discrimination index in the object recognition test. After the MWM test and the object recognition test, animals were sacrificed and the brain tissues were collected. Aβ42 levels in the cortex (K) and hippocampus (L) were detected by ELISA. # p < 0.05, ###p < 0.001 vs. Wt group. * p < 0.05, **p < 0.01, ***p < 0.001 vs. APP/PS1 group.
    PMID: 30128052

靶點詳情

  • 最新研究進展：
    Aβ1-42是β淀粉樣蛋白（amyloid beta protein，Aβ）的一種亞型，它是導致阿爾茨海默?。ˋlzheimer's disease，AD）的主要病理因素之一。最新的研究表明，Aβ1-42的聚集和沉積不僅在AD中發(fā)揮作用，而且在其他神經(jīng)退行性疾病中也可能具有重要作用，如前額顳葉癡呆癥、帕金森氏病等。