Pig interleukin 10,IL-10 ELISA Kit
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中文名稱:豬白介素10(IL-10)酶聯(lián)免疫試劑盒
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貨號(hào):CSB-E06779p
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規(guī)格:96T/48T
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價(jià)格:¥3600/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:
This Pig IL10 ELISA Kit was designed for the quantitative measurement of Pig IL10 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 6.25 pg/mL-400 pg/mL and the sensitivity is 1.56 pg/mL .
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別名:IL10Interleukin-10 ELISA Kit; IL-10 ELISA Kit; Cytokine synthesis inhibitory factor ELISA Kit; CSIF ELISA Kit
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縮寫:
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Uniprot No.:
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種屬:Sus scrofa (Pig)
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樣本類型:serum, plasma, tissue homogenates
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檢測(cè)范圍:6.25 pg/mL-400 pg/mL
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靈敏度:1.56 pg/mL
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反應(yīng)時(shí)間:1-5h
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樣本體積:50-100ul
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檢測(cè)波長(zhǎng):450 nm
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研究領(lǐng)域:Immunology
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測(cè)定原理:quantitative
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測(cè)定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 90 Range % 81-103 1:2 Average % 95 Range % 90-105 1:4 Average % 95 Range % 90-108 1:8 Average % 96 Range % 82-112 -
回收率:
The recovery of pig IL-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 95 85-109 EDTA plasma (n=4) 97 92-106 -
標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average Corrected 400 2.178 2.152 2.165 2.015 200 1.584 1.560 1.572 1.422 100 0.981 0.950 0.966 0.816 50 0.568 0.564 0.566 0.416 25 0.372 0.361 0.367 0.217 12.5 0.296 0.302 0.299 0.149 6.25 0.252 0.248 0.250 0.100 0 0.152 0.148 0.150 -
數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻(xiàn)
- β-hydroxybutyrate administration improves liver injury and metabolic abnormality in postnatal growth retardation piglets C Wang,Frontiers in veterinary science,2023
- Comparative Analysis of Intestinal Inflammation and Microbiota Dysbiosis of LPS-Challenged Piglets between Different Breeds C Li,Animals,2024
- Effects of chlortetracycline on growth performance and intestinal functions in weaned piglets H Han,Journal of applied microbiology,2023
- Recombinant characteristics, pathogenicity, and viral shedding of a novel PRRSV variant derived from twice inter-lineage recombination XY Cui,Veterinary microbiology,2023
- Dietary Litsea cubeba essential oil supplementation improves growth performance and intestinal health of weaned piglets Z Yang,Animal Nutrition,2022
- A water-soluble beta-glucan improves growth performance by altering gut microbiome and health in weaned pigs Y Wu,Animal Nutrition,2021
- Eficacia comparativa de la administración simultánea de una vacuna de circovirus porcino tipo 2 (PCV2) más una vacuna del virus del síndrome reproductivo … J Jeong,/,2020
- Streptococcus suis Serotype 2 Infection Causes Host Immunomodulation Through Induction of Thymic Atrophy Wang S,Infect Immun,2020
- Oral administration of Lactobacillus delbrueckii during the suckling period improves intestinal integrity after weaning in piglets Yinghui Li, et al,Journal of Functional Foods,2019
- Cathepsin L promotes secretory IgA response by participating in antigen presentation pathways during Mycoplasma Hyopneumoniae infection Zhang N, et al,PLoS One.,2019
- Probiotics-fermented Massa Medicata Fermentata ameliorates weaning stress in piglets related to improving intestinal homeostasis Yanbo Wang, et al,Applied Microbiology and Biotechnology,2018
- Effects of dietary lysozyme levels on growth performance, intestinal morphology, immunity response and microbiota community of growing pigs Lijun Zou.et al,Journal of the Science of Food and Agriculture,2018
- Influences of Copper/Zinc-Loaded Montmorillonite on Growth Performance, Mineral Retention, Intestinal Morphology, Mucosa Antioxidant Capacity Jiao LF.et al,Biol Trace Elem Res,2018
- Farm-level prevalence and risk factors for detection of hepatitis E virus, porcine enteric calicivirus, and rotavirus in Canadian finisher pigs Barbara Wilhelm.et al,CAN J VET RES.,2016
- of a porcine circovirus type 2 (PCV2) vaccine plus a porcine reproductive and respiratory syndrome virus (PRRSV) vaccine from two commercial sources in pigs Jeong J.et al,Journal of Swine Health and Production,2016
- Effects of yeast products on the intestinal morphology,barrier function, cytokine expression, and antioxidant system of weaned piglets Huan-sheng YANG.et al,Zhejiang University-SCIENCE B,2016
- Effect of low dosage of chito-oligosaccharide supplementation on intestinal morphology, immune response, antioxidant capacity, and barrier function in weaned piglets Xiong X.et al,J Anim Sci.,2015
- Mouse Model Infected Porcine Circovirus Types 2 Gang Liu et al,Journal of Animal and Veterinary Advances,2012
產(chǎn)品評(píng)價(jià)
樣品類型:細(xì)胞培養(yǎng)上清液
樣品信息:其他
稀釋比:沒(méi)有稀釋
產(chǎn)品評(píng)價(jià): 結(jié)果很滿意,也是對(duì)比了別家的試劑盒,最后發(fā)現(xiàn)還是華美的好用,我用的是細(xì)胞培養(yǎng)基上清,別家的試劑盒有干擾因素,華美的沒(méi)有,試了好幾家,也就這個(gè)牌子的好用。
By 高老師
樣品類型:血清
樣品信息:其他 豬
稀釋比:沒(méi)有稀釋
產(chǎn)品評(píng)價(jià): 標(biāo)曲很好,結(jié)果在標(biāo)曲范圍之內(nèi),符合預(yù)期結(jié)果。
By 謝老師
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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功能:Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha. Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation. In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling.
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基因功能參考文獻(xiàn):
- The PRRSV vaccination induced higher levels of IL-10 expression in the first week and this may be why the CSFV vaccination is immunosuppressed. PMID: 27938685
- These data indicate that the type 2 porcine reproductive and respiratory syndrome virus N protein plays an important role in IL-10 induction and the N-N non-covalent domain is associated with this activity. PMID: 25614594
- The effects of semen, spermatozoa in extender, or extender alone on the expression of TGF-beta1, IL-10, and IL-6 in ovarian follicles are reported. PMID: 24020676
- Prostaglandin E2 potentiates mesenchymal stem cell-induced IL-10+IFN-gamma+CD4+ regulatory T cells to control transplant arteriosclerosis. PMID: 23359497
- Porcine reproductive and respiratory syndrome virus strain CH-1a could significantly up-regulate IL-10 production through p38 MAPK activation. PMID: 22909062
- Boar seminal plasma contained TGF- beta1 and IL-10 but with high individual variation. PMID: 22482797
- Changes in interleukin-10 mRNA expression are predictive for 9-day survival of pigs in an emergency preservation and resuscitation model. PMID: 20163907
- These results suggest that TNF-alpha and IL-10, but not IL-6, are involved in the late reparatory phases of the experimental disk lesion. PMID: 19605905
- The present study shows that elevated levels of serum CRP and IL-10 were associated with porcine circovirus type 2-infected piglets that subsequently developed severe PMWS.(postweaning multisystemic wasting syndrome) PMID: 16817761
- Subclinically porcine circovirus type 2 (PCV2)-infected pigs developed a transient PCV2-specific IL-10 response during the viremic phase of infection which coincided with the inversion of the IgM/IgG ratio. PMID: 17592737
- Twenty one polymorphisms in the IL10 gene have been found, including single nucleotide polymorphisms and insertion deletion polymorphisms. The chromosomal location has been mapped by FISH and radiation hybrid mapping. PMID: 18607788
- findings show that Brachyspira hyodysenteriae inoculation induced production of systemic levels of IL-1beta during the dysentery period and increased levels of IL-10 coincided with recovery from dysentery PMID: 18700003
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亞細(xì)胞定位:Secreted.
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蛋白家族:IL-10 family
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