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  4. Mouse 5-Hydroxytryptamine,5HT ELISA Kit

Mouse 5-Hydroxytryptamine,5HT ELISA Kit

  • 中文名稱：
    小鼠5羥色胺(5-HT)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E08365m
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：

    This Mouse 5HT ELISA Kit was designed for the quantitative measurement of Mouse 5HT protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 250 pg/mL-4000 pg/mL and the sensitivity is 125 pg/mL.

  • 縮寫：
    5HT
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    250 pg/mL-4000 pg/mL
  • 靈敏度：
    125 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Neuroscience
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse 5HT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:100 Average % 95  
    Range % 90-89  
    1:200 Average % 90  
    Range % 86-94  
    1:400 Average % 84  
    Range % 80-88  
    1:800 Average % 97  
    Range % 92-101  
  • 回收率：
    The recovery of mouse 5HT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 92 87-99  
    EDTA plasma (n=4) 89 83-96  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    4000 0.130 0.120 0.125    
    2000 0.243 0.241 0.242    
    1000 0.452 0.419 0.436    
    500 0.792 0.831 0.812    
    250 1.068 1.120 1.094    
    0 2.151 2.026 2.089    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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