Human 5-Hydroxytryptamine,5HT ELISA Kit
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中文名稱:人5羥色胺(5-HT)酶聯(lián)免疫試劑盒
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貨號:CSB-E08363h
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規(guī)格:96T/48T
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價格:¥3600/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:
This Human 5HT ELISA Kit was designed for the quantitative measurement of Human 5HT protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 4 ng/mL-800 ng/mL and the sensitivity is 2 ng/mL.
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縮寫:5HT
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種屬:Homo sapiens (Human)
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樣本類型:serum, plasma, tissue homogenates
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檢測范圍:4 ng/mL-800 ng/mL
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靈敏度:2 ng/mL
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反應(yīng)時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領(lǐng)域:Neuroscience
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測定原理:quantitative
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測定方法:Competitive
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精密度:
Intra-assay Precision (Precision within an assay): CV%<15% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<15% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human 5-HT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 96 Range % 92-102 1:2 Average % 94 Range % 91-98 1:4 Average % 85 Range % 80-89 1:8 Average % 95 Range % 89-99 -
回收率:
The recovery of human 5-HT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 95 90-99 EDTA plasma (n=4) 89 85-96 -
標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. ng/ml OD1 OD2 Average 800 0.215 0.216 0.216 200 0.383 0.391 0.387 64 0.575 0.567 0.571 16 0.986 0.974 0.980 4 1.571 1.472 1.522 0 2.444 2.345 2.395 -
數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻(xiàn)
- Early life stress disrupts intestinal homeostasis via NGF-TrkA signaling Wong HLX, et al,Nature Communications,2019
- Essential Fatty Acids Linoleic Acid and Α-\Linolenic Acid Sex-\Dependently Regulate Glucose Homeostasis in Obesity Pan Zhuang.et al,MOL NUTR FOOD RES,2018
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