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Chicken Epidermal growth factor,EGF ELISA Kit

  • 中文名稱(chēng)：
    雞表皮生長(zhǎng)因子(EGF)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E09868Ch
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：

    This Chicken EGF ELISA Kit was designed for the quantitative measurement of Chicken EGF protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 125 pg/mL-8000 pg/mL and the sensitivity is 31.25 pg/mL.

  • 別名：
    N/A
  • 縮寫(xiě)：
  • 種屬：
    Gallus gallus (Chicken)
  • 樣本類(lèi)型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    125 pg/mL-8000 pg/mL
  • 靈敏度：
    31.25 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Others
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of chicken EGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %102
    Range %100-104
    1:2Average %95
    Range %92-98
    1:4Average %88
    Range %85-91
    1:8Average %99
    Range %96-102
  • 回收率：
    The recovery of chicken EGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 104102-106
    EDTA plasma (n=4)9390-96
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    80002.337 2.466 2.402 2.289
    40001.893 1.875 1.884 1.771
    20001.452 1.455 1.454 1.341
    10001.064 1.099 1.082 0.969
    5000.643 0.632 0.638 0.525
    2500.443 0.478 0.461 0.348
    1250.272 0.295 0.284 0.171
    00.114 0.112 0.113
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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