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Pig Epithelial growth factor,EGF ELISA Kit

  • 中文名稱:
    豬表皮生長因子(EGF)酶聯(lián)免疫試劑盒
  • 貨號:
    CSB-E06788p
  • 規(guī)格:
    96T/48T
  • 價格:
    ¥3200/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:

    This Pig EGF ELISA Kit was designed for the quantitative measurement of Pig EGF protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 250 pg/mL-10000 pg/mL and the sensitivity is 125 pg/mL.

  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Sus scrofa (Pig)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    250 pg/mL-10000 pg/mL
  • 靈敏度:
    125 pg/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Others
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of pig EGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 100  
    Range % 97-105  
    1:2 Average % 93  
    Range % 90-96  
    1:4 Average % 102  
    Range % 98-106  
    1:8 Average % 87  
    Range % 83-90  
  • 回收率:
    The recovery of pig EGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 86 83-89  
    EDTA plasma (n=4) 101 99-104  
                 
                 
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    10000 2.647 2.687 2.667    
    5000 1.839 1.899 1.869    
    2500 1.057 1.067 1.062    
    1250 0.599 0.584 0.592    
    500 0.241 0.231 0.236    
    250 0.112 0.114 0.113    
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

產(chǎn)品評價