IRAK4

IRAK4，即白細(xì)胞介素-1受體相關(guān)激酶4，別名IL-1受體相關(guān)激酶4。它是一種在Toll樣受體（TLRs）和IL-1受體介導(dǎo)的炎癥發(fā)生中的關(guān)鍵性蛋白。IRAK4由人體細(xì)胞自身的基因編碼，其作用機(jī)制主要是通過參與Toll樣受體信號通路，激活下游的NF-κB和AP-1轉(zhuǎn)錄因子，從而誘導(dǎo)炎癥因子、細(xì)胞因子、趨化因子的表達(dá) 。IRAK4在生物學(xué)上的意義非常重要，它不僅參與了炎癥反應(yīng)的正向調(diào)節(jié)，還廣泛參與了細(xì)胞內(nèi)反應(yīng)的網(wǎng)絡(luò)性調(diào)節(jié)。IRAK4的功能異常與多種炎癥性疾病的發(fā)生發(fā)展有關(guān)，包括類風(fēng)濕性關(guān)節(jié)炎、特應(yīng)性皮炎、化膿性汗腺炎等。此外，IRAK4的過表達(dá)可以增強(qiáng)LPS誘導(dǎo)的NADPH氧化酶活性，誘導(dǎo)下游的p38 MAPK的信號轉(zhuǎn)導(dǎo)，進(jìn)一步擴(kuò)展了IRAK4的生物學(xué)功能。因此，IRAK4是抗炎癥和腫瘤研究中的一個重要靶點，針對IRAK4的小分子抑制劑和蛋白降解劑正在被開發(fā)用于治療相關(guān)疾病。

熱銷產(chǎn)品

IRAK4 Recombinant Monoclonal Antibody (CSB-RA284992A0HU)

驗證數(shù)據(jù)

Western Blot
Positive WB detected in: THP-1 whole cell lysate, U251 whole cell lysate
All lanes: IRAK4 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 52, 38 kDa
Observed band size: 55 kDa

Overlay histogram showing Jurkat cells stained with CSB-RA284992A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1μg/1*10⁶ cells) for 1 h at 4℃.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4℃. Control antibody (green line) was Rabbit IgG (1μg/1*10⁶ cells) used under the same conditions. Acquisition of >10,000 events was performed.

IRAK4 Antibody (CSB-PA011812ESR1HU)

驗證數(shù)據(jù)

Western blot
All lanes: Interleukin-1 receptor-associated kinase 4 antibody at 4μg/ml + Jurkat whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 52, 38 kDa
Observed band size: 52 kDa

Immunoprecipitating IRAK4 in MCF-7 whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA011812ESR1HU in MCF-7 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA011812ESR1HU (8μg) + MCF-7 whole cell lysate (500μg)
Lane 3: MCF-7 whole cell lysate (10μg)

IRAK4 Antibody

IRAK4 Protein

IRAK4 ELISA Kit

IRAK4 Antibodies

IRAK4 for Homo sapiens (Human)

產(chǎn)品貨號	產(chǎn)品名稱	種屬反應(yīng)性	應(yīng)用類型
CSB-PA011812ESR1HU	IRAK4 Antibody	Human	ELISA, WB, IP
CSB-PA011812ESR2HU	IRAK4 Antibody	Human	ELISA
CSB-PA793788	IRAK4 Antibody	Human,Mouse	ELISA,IHC
CSB-RA284992A0HU	IRAK4 Recombinant Monoclonal Antibody	Human	ELISA, WB, FC

IRAK4 Proteins

IRAK4 Proteins for Mus musculus (Mouse)

產(chǎn)品貨號	產(chǎn)品名稱	來源
CSB-YP011812MO CSB-EP011812MO CSB-BP011812MO CSB-MP011812MO CSB-EP011812MO-B	Recombinant Mouse Interleukin-1 receptor-associated kinase 4 (Irak4)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

IRAK4 Proteins for Homo sapiens (Human)

產(chǎn)品貨號	產(chǎn)品名稱	來源
CSB-YP011812HU CSB-EP011812HU CSB-BP011812HU CSB-MP011812HU CSB-EP011812HU-B	Recombinant Human Interleukin-1 receptor-associated kinase 4 (IRAK4)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

IRAK4 Proteins for Bos taurus (Bovine)

產(chǎn)品貨號	產(chǎn)品名稱	來源
CSB-YP011812BO CSB-EP011812BO CSB-BP011812BO CSB-MP011812BO CSB-EP011812BO-B	Recombinant Bovine Interleukin-1 receptor-associated kinase 4 (IRAK4)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli