SARS-CoV-2抗體
新冠病毒引起的新冠肺炎疫情此起彼伏，對全球的醫(yī)療衛(wèi)生系統(tǒng)和經(jīng)濟(jì)帶來了巨大影響，同時(shí)也給新冠疫苗、治療藥物以及診斷帶來了新的挑戰(zhàn)。武漢華美生物一直密切關(guān)注新冠病毒的進(jìn)化情況，目前已開發(fā)了大量高質(zhì)量SARS-CoV-2相關(guān)抗體試劑，包括N抗體、S抗體、S RBD納米抗體、N抗體對和S RBD抗體對等。
其中，N抗體與S抗體可用于新冠病毒致病性機(jī)理研究，可通過WB定性檢測新冠抗原；N抗體對和S RBD抗體對可用于新冠試劑盒的開發(fā)；另外，部分抗體驗(yàn)證了中和試驗(yàn)和主流突變株檢測，可作為質(zhì)控品，應(yīng)用于針對突變株的中和抗體篩選、重組蛋白疫苗研發(fā)、疫苗接種后血清中的中和抗體檢測等。
華美特色
- 抗體試劑類型多
- 高特異性
- 多種應(yīng)用場景驗(yàn)證
- 多個(gè)主流突變株驗(yàn)證
部分應(yīng)用場景及驗(yàn)證數(shù)據(jù)
● WB定性抗原檢測
Positive WB detected in: His tag-tagged SARS-CoV-2 N recombinant protein (CSB-EP3325GMY); SARS-CoV-2 N antibody at 1:1000 (CSB-RA33255A1GMY)
Positive WB detected in: SARS-CoV-2 N recombinant protein (CSB-EP3325GMY) at 40ng, 20ng, 10ng; All lanes N antibody (CSB-MA33255A0m) at 1:1000
● 功能性ELISA驗(yàn)證抗原-抗體結(jié)合活性
Immobilized SARS-CoV-2-N (CSB-EP3325GMY) at 2 μg/mL can bind SARS-CoV-2-N (CSB-RA33255A0GMY) Antibody, the EC50 is 6.892 ng/mL.
Immobilized SARS-CoV-2-S1-RBD (CSB-YP3324GMY1) at 2 μg/ml can bind SARS-CoV-2-S Antibody (CSB-MA33245A0m), the EC50 is 32.59 to 39.33 ng/mL.
● 用于膠體金（GICA）快速檢測
In the GICA detection system, the background of antibody (CSB-RA33255A2GMY) is clean, the detection limit can be as low as 15.625ng/mL (1.09ng/0.07mL), and the sensitivity is very good.
In the GICA detection system, the background of antibody (CSB-RA33245A0GMY) is clean, the detection limit can be as low as 13.94ng/mL (0.976ng/0.07mL), and the sensitivity is very good.
● 抗體對經(jīng)S-ELISA驗(yàn)證
SARS-CoV-2 N Antibody Pair 1 (CSB-EAP33255A1) is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA).
SARS-CoV-2 Spike RBD Antibody Pair 1 (CSB-EAP33245) is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA).
● 經(jīng)中和試驗(yàn)驗(yàn)證中和抗體
Binding signal of SARS-CoV-2-S1-RBD (CSB-YP3324GMY1) and ACE2 protein-HRP conjugate (CSB-MP866317HU) was inhibited by S Antibody (CSB-RA33245A1GMY) with the IC50 is 2.38 μg/mL
Binding signal of SARS-CoV-2-S1-RBD (CSB-YP3324GMY1) and ACE2-HRP (CSB-YP3324GMY1) was inhibited by SARS-CoV-2 Spike RBD Nanobody (CSB-RA33245A2GMY) with the IC50 is 0.1074 μg/mL.
● 多個(gè)突變株識(shí)別驗(yàn)證
| Protein Code | Variants | EC50 (ng/mL) |
|---|---|---|
| CSB-EP3325GMY(M1) | D3L, R203K, G204R, S235F | 2. 562-3.421 |
| CSB-EP3325GMY(M2) | T205I | 29.34-40.67 |
| CSB-EP3325GMY(M3) | P80R | 63.78-113.6 |
| CSB-EP3325GMY(M4) | P13L | 2.825-4.124 |
| CSB-EP3325GMY(M5) | D103Y | 7.358-9.976 |
| CSB-EP3325GMY(M8) | S202N | 1.466-2.382 |
| CSB-EP3325GMY(M9) | L230F | 4.854-7.721 |
| CSB-EP3325GMY(M10) | I292T | 3.859-7.358 |
| CSB-EP3325GMY(M11) | Q384H | 2.785-4.100 |
| CSB-EP3325GMY(M12) | R203K, G204R | 4.664-8.256 |
| CSB-EP3325GMY(M13) | R203M, D377Y | 0.8488-1.385 |
| CSB-EP3325GMY(M14) | D63G, R203M, D377Y | 1.304-1.875 |
The Binding Activity of N protein with N Recombinant Antibody. Activity: Measured by its binding ability in a functional ELISA. Immobilized N at 2 μg/mL can bind N Recombinant Antibody (CSB-RA33255A1GMY), the EC50 of N Recombinant Antibody.
| Protein Code | Variants | EC50 (ng/mL) |
|---|---|---|
| CSB-MP3324GMY1(M8) | E484K | 371.0-487.5 |
| CSB-MP3324GMY1(M7) | K417N | 4.522-5.950 |
| CSB-MP3324GMY1(M6) | N501Y | 47.09-86.25 |
| Protein Code | Variants | EC50 (ng/mL) |
|---|---|---|
| CSB-MP3324GMY1(M9) | K417T | 8.706-13.61 |
| CSB-MP3324GMY1(M10) | K417N, E484K, N501Y | 97.92-169.6 |
| CSB-MP3324GMY(M1) | D614G | 23.19-34.02 |
The Binding Activity of S protein with S Recombinant Antibody. Activity: Measured by its binding ability in a functional ELISA. Immobilized S at 2 μg/mL can bind S Recombinant Antibody, the EC50 of S Recombinant Antibody (CSB-RA33245A1GMY).
產(chǎn)品列表
● N antibody
| 貨號(hào) | 產(chǎn)品名稱 | 反應(yīng)種屬 | 應(yīng)用類型 |
|---|---|---|---|
| CSB-RA33255A1GMY | N Antibody | SARS-CoV-2 | ELISA, WB, GICA |
| CSB-RA33255A2GMY | N Antibody | SARS-CoV-2 | ELISA |
| CSB-MA33255A0m | N Antibody | GICA | Mouse |
| CSB-PA33254A0Rb | N Antibody | GICA | Rabbit |
| CSB-RA33255A0GMY | N Antibody | SARS-CoV-2 | ELISA, GICA |
| CSB-RA33255B1GMY | N Antibody, HRP conjugated | SARS-CoV-2 | ELISA |
| CSB-RA33255C1GMY | N Antibody, FITC conjugated | SARS-CoV-2 | ELISA |
| CSB-RA33255D1GMY | N Antibody, Biotin conjugated | SARS-CoV-2 | ELISA |
| CSB-MA33255A1m | N Antibody | GICA | Mouse |
| CSB-MA33255A2m | N Antibody | GICA | Mouse |
| CSB-PA33254B0Rb | N Antibody, HRP conjugated | SARS-CoV-2 | ELISA |
| CSB-PA33254C0Rb | N Antibody, FITC conjugated | SARS-CoV-2 | ELISA |
| CSB-PA33254D0Rb | N Antibody, Biotin conjugated | SARS-CoV-2 | ELISA |
● S antibody
| 貨號(hào) | 產(chǎn)品名稱 | 反應(yīng)種屬 | 應(yīng)用類型 |
|---|---|---|---|
| CSB-RA33245A1GMY | S Antibody | SARS-CoV-2 | ELISA, GICA, Neutralising |
| CSB-MA33245A2m | S Monoclonal Antibody | GICA | Mouse |
| CSB-PA33245YA01GMY | S Antibody | GICA | Rabbit |
| CSB-RA33245A0GMY | S Antibody | SARS-CoV-2 | ELISA, GICA |
| CSB-RA33245B1GMY | S Antibody, HRP conjugated | SARS-CoV-2 | ELISA |
| CSB-RA33245C1GMY | S Antibody, FITC conjugated | SARS-CoV-2 | ELISA |
| CSB-RA33245D1GMY | S Antibody, Biotin conjugated | SARS-CoV-2 | ELISA |
| CSB-MA33245A0m | S Monoclonal Antibody | GICA | Mouse |
| CSB-MA33245A1m | S Monoclonal Antibody | GICA | Mouse |
| CSB-PA33245YB01GMY | S Antibody | SARS-CoV-2 | ELISA |
| CSB-PA33245YC01GMY | S Antibody | SARS-CoV-2 | ELISA |
| CSB-PA33245YD01GMY | S Antibody | SARS-CoV-2 | ELISA |
| CSB-PA33245YA11GMY | S Antibody | GICA | Rabbit |
| CSB-PA33245YB11GMY | S Antibody | SARS-CoV-2 | ELISA |
| CSB-PA33245YC11GMY | S Antibody | SARS-CoV-2 | ELISA |
| CSB-PA33245YD11GMY | S Antibody | SARS-CoV-2 | ELISA |
● N Antibody Pair
| 貨號(hào) | 產(chǎn)品名稱 | 反應(yīng)種屬 | 應(yīng)用類型 |
|---|---|---|---|
| CSB-EAP33255A2 | SARS-CoV-2 N Antibody Pair 2 | SARS-CoV-2 | S-ELISA |
| CSB-EAP33255A1 | SARS-CoV-2 N Antibody Pair 1 | SARS-CoV-2 | S-ELISA |
| CSB-EAP33255A3 | SARS-CoV-2 N Antibody Pair 3 | SARS-CoV-2 | S-ELISA |
| CSB-EAP33255A4 | SARS-CoV-2 N Antibody Pair 4 | SARS-CoV-2 | S-ELISA |
● S RBD Nanobody Antibody
| 貨號(hào) | 產(chǎn)品名稱 | 反應(yīng)種屬 | 應(yīng)用類型 |
|---|---|---|---|
| CSB-RA33245A2GMY | SARS-CoV-2 RBD Nanobody | SARS-CoV-2 | ELISA, GICA, Neutralising |
| CSB-RA33245B2GMY | SARS-CoV-2 RBD Nanobody, HRP conjugated | SARS-CoV-2 | ELISA |
| CSB-RA33245C2GMY | SARS-CoV-2 RBD Nanobody, FITC conjugated | SARS-CoV-2 | ELISA |
| CSB-RA33245D2GMY | SARS-CoV-2 RBD Nanobody, Biotin conjugated | SARS-CoV-2 | ELISA |
● S RBD Antibody Pair
| 貨號(hào) | 產(chǎn)品名稱 | 反應(yīng)種屬 | 應(yīng)用類型 |
|---|---|---|---|
| CSB-EAP33245 | SARS-CoV-2 Spike RBD Antibody Pair 1 | SARS-CoV-2 | S-ELISA |
