Recombinant Rat Osteocalcin (Bglap)
In Stock-
中文名稱(chēng):Recombinant Rat Osteocalcin(Bglap)
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貨號(hào):CSB-EP002682RA
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規(guī)格:¥1836
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圖片:
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其他:
產(chǎn)品詳情
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純度:Greater than 90% as determined by SDS-PAGE.
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基因名:Bglap
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Uniprot No.:
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別名:Bglap; Bglap2; Osteocalcin; Bone Gla protein; BGP; Gamma-carboxyglutamic acid-containing protein
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種屬:Rattus norvegicus (Rat)
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蛋白長(zhǎng)度:Full Length of Mature Protein
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來(lái)源:E.coli
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分子量:32.6kDa
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表達(dá)區(qū)域:50-99aa
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氨基酸序列YLNNGLGAPAPYPDPLEPHREVCELNPNCDELADHIGFQDAYKRIYGTTV
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request. -
蛋白標(biāo)簽:N-terminal GST-tagged
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產(chǎn)品提供形式:Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand. -
緩沖液:Tris-based buffer,50% glycerol
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儲(chǔ)存條件:Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
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保質(zhì)期:The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. -
貨期:3-7 business days
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注意事項(xiàng):Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
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產(chǎn)品描述:重組大鼠骨鈣素(Bglap),也稱(chēng)為骨γ-羧基谷氨酸蛋白,是一種在骨細(xì)胞中高度表達(dá)的蛋白質(zhì),可以通過(guò)重組技術(shù)來(lái)生產(chǎn)。下面是一般的步驟:設(shè)計(jì)和合成基因:基于大鼠骨鈣素的氨基酸序列,設(shè)計(jì)并合成含有Bglap基因的DNA序列。這可以通過(guò)基因合成公司或PCR方法完成。克隆基因:將Bglap基因插入到表達(dá)載體中,這可以通過(guò)PCR擴(kuò)增和限制性?xún)?nèi)切酶切割的方法完成。建議選擇一些經(jīng)過(guò)驗(yàn)證的表達(dá)載體,如pET系列載體,這些載體中包含了一些促進(jìn)蛋白表達(dá)的元件,如啟動(dòng)子、增強(qiáng)子、終止子等。轉(zhuǎn)染宿主細(xì)胞:在細(xì)胞中轉(zhuǎn)染含有Bglap基因的表達(dá)載體。這些宿主細(xì)胞可以選擇常見(jiàn)的哺乳動(dòng)物細(xì)胞系,如CHO細(xì)胞、HEK293細(xì)胞等。培養(yǎng)和表達(dá)蛋白:根據(jù)表達(dá)載體中的啟動(dòng)子和調(diào)節(jié)元件,誘導(dǎo)Bglap蛋白在轉(zhuǎn)染的宿主細(xì)胞中表達(dá)。在培養(yǎng)期間,需要添加抗生素等來(lái)保持表達(dá)載體的穩(wěn)定。蛋白質(zhì)的純化:通過(guò)細(xì)胞破碎和離心等方法提取含有Bglap蛋白的細(xì)胞裂解液。然后使用親和層析、離子交換層析、凝膠過(guò)濾層析等方法對(duì)蛋白進(jìn)行純化。蛋白質(zhì)的鑒定和活性檢測(cè):使用SDS-PAGE、Western blot等方法對(duì)純化后的Bglap蛋白進(jìn)行鑒定和定量。此外,可以使用免疫印跡、酶聯(lián)免疫吸附試驗(yàn)(ELISA)等方法來(lái)檢測(cè)其生物學(xué)活性和功能。
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Datasheet & COA:Please contact us to get it.
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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功能:Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium.
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基因功能參考文獻(xiàn):
- These data suggest that Undercarboxylated osteocalcin may have other effects on skeletal muscle in addition to its insulin sensitizing effect. PMID: 27291707
- Uncarboxylated osteocalcin potentiates insulin secretion, inhibits Kv channels and increases [Ca(2+)]i. PMID: 27746193
- This rat model of complete loss of osteocalcin provides a platform for further understanding the role of osteocalcin in disease. PMID: 27483347
- gamma-Carboxylase inhibition prevents osteocalcin carboxylation and protects against diabetic cardiomyopathy, possibly through direct action on upregulated G protein-coupled receptor family C group 6 member A and indirectly via adiponectin. PMID: 27488359
- Bone Gla Protein PMID: 25185647
- In primary adipocytes, both carboxylated and uncarboxylated osteocalcin increased basal and insulin-stimulated glucose transport. PMID: 24554534
- The physiological changes associated with estrous cycle stage were not sufficient to cause a detectable difference in carboxylated or undercarboxylated osteocalcin concentrations in this study. PMID: 23817840
- Osteocalcin (OC)-containing neurons are distributed throughout the cerebellar nuclei; OC may play a role in modulating the neuroprotective function of osteopontin. PMID: 22552891
- warfarin impaired the carboxylation of osteocalcin in rats. Factor Xa inhibitor edoxaban at or higher doses than needed for an antithrombotic effect sustained the circulating Gla-osteocalcin level. PMID: 22999414
- results suggest that under some physiological conditions the oncoprotein MDM2 may cooperate with p53 to regulate the osteocalcin gene during osteoblastic differentiation. PMID: 22405968
- These data are strongly in support of Sp1 as an essential transcription factor required for Osx recruitment and transactivation of the OCN promoter. PMID: 21820092
- purified Bone Gla Protein (BGP) resulted in stabilization of hypoxia-inducible factor 1alpha (HIF-1alpha) in chondrocytes and vascular smooth muscle cells PMID: 21757657
- The mechanism of high glucose induced calcification in vascular smooth muscle cells may be due to the increased expression of cbfalpha-1 and OC. PMID: 21302441
- functional link between vitamin D-dependent bone tissue-specific transcription and histone acetylation at osteocalcin locus PMID: 11893738
- the VDRE in the distal region of the OC gene promoter is refractory to binding of the VDR-RXR complex when organized in a nucleosomal context PMID: 11964167
- deltaEF1 suppresses expression of the endogenous osteocalcin gene PMID: 12193549
- acetylation of histones H3 and H4 is functionally coupled to the chromatin remodeling events that mediate the developmental induction of osteocalcin gene transcription in bone cells PMID: 12554783
- Type I collagen promotes the activity of dentin formation by dental pulp cells by down-regulating Dmp-1 gene expression and up-regulating osteocalcin gene expression. PMID: 12647294
- A human vitamin D receptor-glucocorticoid receptor DNA-binding domain chimera is functional on rat osteocalcin vitamin D-responsive element with only conservative change of lys-49 to arg, and of negatively charged glu-53 to basic amino acid (lys or arg). PMID: 12960019
- Data show that in osteoblastic cells, the vitamin D receptor interacts directly with Runx2, and that this interaction contributes significantly to vitamin D3-dependent enhancement of the osteocalcin promoter. PMID: 15456860
- Results describe gene regulation by Dlx3 in relation to that of Msx2 and Dlx5 during osteoblast differentiation. PMID: 15456894
- Ocn is a Nurr1 target gene, which positions Nurr1 in the core of transcriptional factors regulating osteoblastic gene expression PMID: 15485875
- OC-IR nerve fibers in the circumvallate papilla mainly originated from the petrosal ganglion. PMID: 15621021
- Osteonectin (ON) and osteocalcin (OC) was used as osteoblast markersand assessed using an indirect immunoperoxidase. technique. PMID: 16443258
- CEBP-beta-dependent recruitment of SWI/SNF activity is required for chromatin remodeling and transcriptional activity of the bone-specific osteocalcin gene PMID: 16772287
- specific protein-DNA and protein-protein interactions that occur within the context of the OC gene promoter in osteoblastic cells stabilize the preferential association of the VDR-SRC-1 complex PMID: 17218095
- These results support a model where VDR preferentially recruits SRC-1 to enhance bone-specific OC gene transcription. PMID: 17786964
- These data suggest that long-term reduction in bone OC levels may induce the formation of immature bone, which is easily resorbed with changes in bone metabolism such as ovariectomy, and that OC may be one of the factors affecting bone turnover. PMID: 17968486
- TFIIA gamma together with ATF4 and Runx2 stimulates osteocalcin promoter activity and endogenous mRNA expression. PMID: 18171674
- Biomineralization followed secretion of osteocalcin, which may reflect early osteoblastic differentiation of cultured mesenchymal stem cells under osteoinductive conditions. PMID: 19191495
- The Notch-responsive Hes-1 protein is capable of repressing osteocalcin gene transcription in osteoblastic cells through an E-box in the proximal promoter. PMID: 19670267
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亞細(xì)胞定位:Secreted.
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蛋白家族:Osteocalcin/matrix Gla protein family
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