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Recombinant Mouse CCN family member 1 (Ccn1)

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  • 中文名稱：
    Recombinant Mouse CCN family member 1(Ccn1)
  • 貨號：
    CSB-EP006463MO
  • 規(guī)格：
    ￥1836
  • 圖片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP006463MO could indicate that this peptide derived from E.coli-expressed Mus musculus (Mouse) Cyr61.
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP006463MO could indicate that this peptide derived from E.coli-expressed Mus musculus (Mouse) Cyr61.
  • 其他：

產(chǎn)品詳情

  • 純度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    Cyr61
  • Uniprot No.：
  • 別名：
    Ccn1; Cyr61; Igfbp10; CCN family member 1; 3CH61; Cellular communication network factor 1; Cysteine-rich angiogenic inducer 61; Insulin-like growth factor-binding protein 10; IBP-10; IGF-binding protein 10; IGFBP-10; Protein CYR61
  • 種屬：
    Mus musculus (Mouse)
  • 蛋白長度：
    Full Length of Mature Protein
  • 來源：
    E.coli
  • 分子量：
    42.6 kDa
  • 表達區(qū)域：
    25-379aa
  • 氨基酸序列
    TCPAACHCPLEAPKCAPGVGLVRDGCGCCKVCAKQLNEDCSKTQPCDHTKGLECNFGASSTALKGICRAQSEGRPCEYNSRIYQNGESFQPNCKHQCTCIDGAVGCIPLCPQELSLPNLGCPNPRLVKVSGQCCEEWVCDEDSIKDSLDDQDDLLGLDASEVELTRNNELIAIGKGSSLKRLPVFGTEPRVLFNPLHAHGQKCIVQTTSWSQCSKSCGTGISTRVTNDNPECRLVKETRICEVRPCGQPVYSSLKKGKKCSKTKKSPEPVRFTYAGCSSVKKYRPKYCGSCVDGRCCTPLQTRTVKMRFRCEDGEMFSKNVMMIQSCKCNYNCPHPNEASFRLYSLFNDIHKFRD
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白標簽：
    N-terminal 10xHis-tagged
  • 產(chǎn)品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    Tris-based buffer,50% glycerol
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    3-7 business days
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 產(chǎn)品描述：

    This recombinant Mouse Cyr61 protein is an E.coli?expressed protein (Full Length of Mature Protein) with N-terminal 10xHis tag?and its purity is 90%+ determined by SDS-PAGE. With the appropriate cDNA and PCR methods, Cyr61?expression plasmids can be rapidly produced. which must undergo denaturation and folding cycle, can be recovered with more modest yields. Hence, using small-scale fermentations and laboratory-scale processing equipment, Cyr61 proteins (or subdomains thereof) can usually be produced in sufficient quantities to initiate most studies including detailed structural determinations.which must undergo denaturation and folding cycle, can be recovered with more modest yields. Hence, using small-scale fermentations and laboratory-scale processing equipment, Cyr61 proteins (or subdomains thereof) can usually be produced in sufficient quantities to initiate most studies including detailed structural determinations.

    Cyr61, also called Ccn1, is a growth factor-inducible prompt response transcription product that regulates cell proliferation, survival, differentiation, migration, apoptosis, angiogenesis, adhesion, and synthesis of extracellular matrix (ECM) via cell type-specific binding mainly to α6β1, αMβ2, and αVβ3 integrins. It also plays a vital role in the female reproductive system during embryogenesis and tumorigenesis. Cyr61 is upregulated as an angiogenic factor in the cardiovascular system under pathophysiological conditions, revealing a role for Cyr61 in cellular and tissue self-renewal, potentially involving circulating progenitor cells. Studies have found that Cyr61 limits fibrosis in the liver and inhibits hepatocarcinogenesis by blocking EGFR-dependent hepatocyte compensatory proliferation.

  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up-regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5. CCN1-mediated gene regulation is dependent on heparin-binding. Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1. Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-1/beta-5 and cell proliferation through integrin alpha-v/beta-3.
  • 基因功能參考文獻：
    1. These findings reveal a critical role for CCN1 in restoring mucosal homeostasis after intestinal injury PMID: 25807183
    2. CYR61 is upregulated in acute lung injury (ALI) and CYR61 overexpression exacerbates ALI in mice. PMID: 25713320
    3. CYR61 silencing can blunt the malignant behavior of osteosarcoma tumor cells by limiting primary tumor growth and dissemination process. PMID: 25065593
    4. Knock-in mice expressing a CCN1 mutant that is unable to bind alphavbeta5/alphavbeta3 were impaired in ductular reaction. CCN1, acting through its alphav beta3/alphav beta5 binding site, is critical for survival after bile duct ligation. PMID: 25822023
    5. Findings reveal that CCN1 plays a novel role in pathogen clearance; increasing macrophage infiltration and activation resulting in increased virus clearance in tumors. PMID: 24895995
    6. Extracellular Cyr61 convergence with growth factor signaling and integrin/FAK signaling is a new concept of growth factor-induced cell migration. PMID: 25623072
    7. These findings implicate CCN1 as a mediator of cardioprotection induced by G-protein coupled receptor agonists that activate RhoA/MRTF-A signaling. PMID: 25106095
    8. Our results here reveal a novel role of Cyr61 in the pathogenesis of rheumatoid arthritis PMID: 24517278
    9. Findings suggest that that cysteine-rich 61 protein (cyr61) is expressed in airway epithelial cells and has a positive correlation with eotaxin and number of airway infiltrating eosinophils. PMID: 24142710
    10. It provides evidence that CCN1 is released from activated platelets, suggesting that platelets constitute a novel source for CCN1 release to the circulation during sepsis. PMID: 24430538
    11. Binding of CYR61 to LFA-1, ICAM-1 and integrin alpha6 supports the adhesion of thymic epithelial cells and T-cell. PMID: 24280864
    12. these data reveal that de novo Cyr61 in the extracellular matrix bridges LPA and integrin pathways, which in turn, activate FAK, leading to cell migration. PMID: 24371135
    13. CCN1 protein expression is increased in aging skeletal muscle. PMID: 24196529
    14. The aim of the study was to evaluate CCN1 protein abundance and localization in the kidney of IL-6-deficient C57BL/6J (IL-6 KO) mice. PMID: 23690222
    15. Degradome products of the matricellular protein CCN1 as modulators of pathological angiogenesis in the retina. PMID: 23798676
    16. role of Cyr61 in obstructive kidney fibrosis PMID: 23457573
    17. the potential roles of CCN1 in the inflammation and macrophage infiltration of nonalcoholic fatty liver disease ( PMID: 23071295
    18. the matricellular protein CCN1/Cyr61 is a critical regulator of Sonic Hedgehog in pancreatic carcinogenesis PMID: 23027863
    19. The expressions of Cyr61, MMP-2, and VEGF at the levels of mRNA and protein were decreased in the QYHJ groups. PMID: 21382954
    20. a novel, VEGF-independent, clinically relevant, pro-angiogenic factor, CYR61, that is a transcriptional target of Hh-GLI signaling PMID: 22056874
    21. Cyr61 expression increases with tumor grade in human osteosarcoma and demonstrate that cyr61 silencing inhibits in vitro osteosarcoma cell invasion and migration as well as in vivo lung metastases in mice. PMID: 21312266
    22. newly identified functions of CCN1 suggest its possible therapeutic utility in ischemic retinopathy. PMID: 21212276
    23. extracellular matrix protein CCN1 dictates TNFalpha and FasL cytotoxicity PMID: 21153366
    24. Expression of Cyr61 is induced in fibroblasts through plasmin-induced activation of the protease-activated receptor-1 signaling pathway. PMID: 12231560
    25. These findings establish CYR61 as a novel and essential regulator of vascular development. PMID: 12446788
    26. Pressure overload, ischemia, & neurohormonal factors induce myocardial expression of CCN1, a potent proangiogenic factor. CCN1 may play an important role in the adaptation of the heart to cardiovascular stress. PMID: 15117851
    27. results identify a novel and functionally important binding site for integrin alphavbeta3 and provide a new approach for dissecting alphavbeta3-specific CCN1 functions both in cultured cells and in the organism PMID: 15308622
    28. CCN1 acts through integrin alpha6beta1 and heparan sulfate proteoglycans (HSPGs) to promote specific functions in fibroblasts. PMID: 15322081
    29. Ccn1-null mice are impaired in cardiac valvuloseptal morphogenesis, resulting in severe atrioventricular septal defects. PMID: 17023674
    30. Constitutively active rhFOXO3a gene transduction suppressed rmCYR61 expression in rat VSMC. Suppression of CYR61 is among several mechanisms by which FOXO3a inhibits VSMC proliferation and neointimal hyperplasia. PMID: 17234971
    31. Intracolonic overexpression of CCN1 induced angiogenesis in mouse colon. PMID: 18599605
    32. Caveolin-1 (cav-1)/Cyr61 interaction via integrins represents a novel pathway of Cyr61 signaling involving cav-1-dependent processes, which play a critical role in regulating hyperoxia-induced cell death PMID: 18801924
    33. Fas-dependent hepatic apoptosis induced by an agonistic monoclonal anti-Fas antibody or intragastric administration of alcohol is severely blunted in knock-in mice expressing an apoptosis-defective Ccn1 allele. PMID: 19364818
    34. Data suggest that signal-controlled activation of SRF, MRTF-A, and CBP provides a novel connection between mechanical stimuli and CCN1 gene expression. PMID: 19542562
    35. The Cyr61 protein, a product of a growth factor-inducible immediate-early gene, regulates chondrogenesis in mouse limb bud mesenchymal cells. PMID: 9441684
    36. Study shows that the interplay between CYR61-CTGF-NOV1 (CCN1) and Wnt5a in endothelial cells and pericytes determines the angiogenic outcome in a model of ischemic retinopathy. Data highlight the significance of CCN1-EC and CCN1-pericyte communication signals in driving physiological and pathological angiogenesis. PMID: 28469167
    37. Mechanical cyclic stretch led to increased Cyr61 mRNA and protein expression, mobilized NF-kappaB from the cytoplasm to the nucleus and increased IL-8 secretion in A549 cells. PMID: 30128877
    38. Study describes how cysteine-rich 61 (CCN1) promotes monocyte migration by upregulating monocyte chemoattractant protein-1 (CCL2) expression in osteoblasts in rheumatoid arthritis (RA) disease. CCN1 could serve as a potential target for RA treatment. PMID: 28341837
    39. Blocking or knockdown CCN1 expression ameliorated skin inflammation and reduced the expression of CCL20 in both imiquimod and IL-23-induced psoriasis-like mouse models. PMID: 28602508
    40. refine the leukocyte recruitment cascade model by introducing endothelium-bound CCN1 as an inflammation mediator and by demonstrating a role for platelets and patrolling Ly6C(low) monocytes in acute vascular inflammation. PMID: 27482114
    41. This study is the first to describe how CCN1 promotes VEGF expression in osteoblasts and increased endothelial progenitor cells angiogenesis in rheumatoid arthritis disease. PMID: 27465842
    42. Data show that cysteine rich protein 61 protein (CCN1) critically mediates doxorubicin (DOX)-induced cardiotoxicity. PMID: 27167338
    43. Our results not only reveal a novel mechanism illustrating the role of Cyr61 in epidermis pathogenesis but also suggest that therapies targeting Cyr61 may represent a novel strategy in the treatment of psoriasis vulgaris. PMID: 27856305
    44. The LPA1-CCN1 axis may be the central control for SMC migration. PMID: 27760754
    45. these data suggest that the WNT/beta-catenin signaling pathway enhances pancreatic cancer development and malignancy in part via up-regulation of CYR61. PMID: 27889647
    46. Lysophosphatidic acid induced time-dependent phosphorylation of serum response factor and CRE-binding protein in in smooth muscle cells, inducing transcription of CCN1. PMID: 28157379
    47. Data show that Cysteine-rich 61 (CYR61) acts as a tumor-promoting gene in pancreatic neuroendocrine tumors. PMID: 26625209
    48. CCN1 is an important regulator of chondrocyte maturation during cartilage development and homeostasis. PMID: 26363286
    49. CCN1 exacerbates tracheal obliterative bronchiolitis by enhancing fibroproliferation via an alphavbeta3-integrin-independent pathway. PMID: 26174800
    50. Data show that RNA silencing of CCN family member 1 protein (CCN1) inhibits retinal neovascularization (RNV) by inhibiting phosphoinositide 3-kinase (PI3K)/AKT protein signaling in mouse pup model of oxygen-induced retinopathy (OIR). PMID: 26459773

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  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    CCN family
  • 組織特異性：
    Low in kidney, adrenal gland, testes, brain, and ovary, moderate in heart, uterus, and skeletal muscle, highest in lung.
  • 數(shù)據(jù)庫鏈接：