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  4. Recombinant Human SARS coronavirus Nucleoprotein (N)

Recombinant Human SARS coronavirus Nucleoprotein (N)

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  • 中文名稱：
  • 貨號：
    CSB-EP349523HQE
  • 規(guī)格：
    ￥1344
  • 促銷：
    現(xiàn)貨重組蛋白特價促銷
  • 圖片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

產(chǎn)品詳情

  • 純度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 別名：
    Nucleocapsid protein
  • 種屬：
    Human SARS coronavirus (SARS-CoV) (Severe acute respiratory syndrome coronavirus)
  • 蛋白長度：
    Full Length
  • 來源：
    E.coli
  • 分子量：
    50.1 kDa
  • 表達區(qū)域：
    1-422aa
  • 氨基酸序列
    MSDNGPQSNQRSAPRITFGGPTDSTDNNQNGGRNGARPKQRRPQGLPNNTASWFTALTQHGKEELRFPRGQGVPINTNSGPDDQIGYYRRATRRVRGGDGKMKELSPRWYFYYLGTGPEASLPYGANKEGIVWVATEGALNTPKDHIGTRNPNNNAATVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRGNSRNSTPGSSRGNSPARMASGGGETALALLLLDRLNQLESKVSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKQYNVTQAFGRRGPEQTQGNFGDQDLIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYHGAIKLDDKDPQFKDNVILLNKHIDAYKTFPPTEPKKDKKKKTDEAQPLPQRQKKQPTVTLLPAADMDDFSRQLQNSMSGASADSTQA
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白標簽：
    N-terminal 6xHis-tagged
  • 產(chǎn)品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    3-7 business days
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. May modulate transforming growth factor-beta signaling by binding host SMAD3.
  • 基因功能參考文獻：
    1. The severe acute respiratory syndrome coronavirus N protein was found to bind to the SPRY domain of the tripartite motif protein 25 (TRIM25) E3 ubiquitin ligase, thereby interfering with the association between TRIM25 and retinoic acid-inducible gene I (RIG-I) and inhibiting TRIM25-mediated RIG-I ubiquitination and activation. PMID: 28148787
    2. Electrostatic repulsion acts as a switch to regulate SARS virus N protein oligomerization. PMID: 23717688
    3. These results suggest that SARS coronavirus could reduce pyruvate kinase activity via its nucleocapsid protein, and this may in turn cause disease. PMID: 22222284
    4. The co-localization of SARS-CoVN and CXCL16 in the cytoplasm of HEK293FT cells was also shown using confocal laser scanning microscopy. PMID: 20960183
    5. The HLA-A 2402 complexed with SARSV N1 showed a novel host strategy to present an immunodominant CTL epitope by intrachain hydrogen bond as a featured epitope. PMID: 20844028
    6. SARS-CoV N interacts with MAP19 and increased the expression of MAP19 in cells. PMID: 19737459
    7. This is the first report showing the ability of the nucleocapsid protein of SARS-CoV to induce apoptosis and actin reorganization in mammalian cells under stressed conditions. PMID: 15294014
    8. analysis of SARS-CoV nucleocapsid protein expressed in insect cells PMID: 15514849
    9. The nucleocapsid protein of the SARS coronavirus was cloned and purified. PMID: 15523835
    10. Binding to cyclophilin A during invasion of host cells. PMID: 15688292
    11. coronavirus N protein undergoes posttranslational modification by sumoylation; functional implication of this modification in the formation of coronavirus ribouncleoprotein complex, virion assembly and virus-host interactions PMID: 15848177
    12. the nucleocapsid protein forms a dimer through its C-terminal domain PMID: 15849181
    13. Differences in nuclear/nucleolar localization properties of N from other members of coronavirus family suggest a unique shuttle protein function for N, which may play an important role in the pathogenesis of SARS. PMID: 15992957
    14. results showed that the SARS-CoV N protein can significantly activate NF-kappaB only in Vero E6 cells, which are susceptible to SARS-CoV infection, but not in Vero or HeLa cells; suggests that NF-kappaB activation is cell-specific PMID: 16143815
    15. The secondary structure of the dimerization domain consists of five alpha helices and a beta-hairpin. PMID: 16214138
    16. Results show that the N protein consists of two non-interacting structural domains, the N-terminal RNA-binding domain (RBD) (residues 45-181) and the C-terminal dimerization domain (residues 248-365) (DD), surrounded by flexible linkers. PMID: 16228284
    17. data suggest that the SR-rich motif of the SARS-CoV N protein might play an import role in the transformation of the SARS-CoV N protein between the dimer and multimer during its binding to its central region for self-association or dissociation PMID: 16285739
    18. cytoplasmic localization was directed by region III and was the dominant localization signal in the N protein. PMID: 16298975
    19. the S phase inhibitory activity of the N protein may have major significance during viral pathogenesis PMID: 16431923
    20. the N protein has a dimer with extensive interactions between the two subunits, suggesting that the dimeric form of the N protein is the functional unit in vivo PMID: 16627473
    21. using the solution structure of severe acute respiratory (SARS) coronavirus N-protein, revealed that this motif is available for interaction with cellular factors which may mediate nucleolar localization PMID: 16734668
    22. This is the first report demonstrating the interaction of hUbc9 with a structural protein of plus-strand RNA viruses, indicating a new drug target for SARS-CoV. PMID: 16998888
    23. the nucleocapsid protein of severe acute respiratory syndrome coronavirus is sumoylated by interaction with Ubc9 PMID: 17037517
    24. characterization of the structures of N protein N-terminal domain in two crystal forms, at 1.17 A (monoclinic) and at 1.85 A (cubic), respectively PMID: 17229691
    25. The C-terminal domain (CTD), spanning residues 248-365 (NP248-365), had stronger nucleic acid-binding activity than the NTD. The crystal structure of the NP248-365 region is solved, suggesting a mechanism for helical RNA packaging in the virus. PMID: 17379242
    26. SARS coronavirus N protein can induce apoptosis of COS-1 cells by activating mitochondrial pathway PMID: 17453707
    27. We also showed that N protein activated IL-6 expression through NF-kappaB by facilitating the translocation of NF-kappaB from cytosol to nucleus. PMID: 17490702
    28. SARS coronavirus nucleocapsid protein interacts with Smad3 and modulates transforming growth factor-beta signaling PMID: 18055455
    29. The experiments revealed that N induces the intrinsic apoptotic pathway, resulting in processing of N at residues 400 and 403 by caspase-6 and/or caspase-3. PMID: 18155731
    30. fgl2 gene transcription induced by the N protein of SARS-CoV was dependent on transcription factor C/EBP alpha binding with its cognate cis-element in fgl2 promoter. PMID: 18390877
    31. It was demonstrated that the N protein of SARS-CoV induces aggregation of EF1, inhibiting protein translation and cytokinesis by blocking F-actin bundling. PMID: 18448518
    32. This study employed the SAIL method to determine the high-quality solution structure of the severe acute respiratory syndrome coronavirus nucleocapsid protein C-terminal domain by NMR. PMID: 18561946
    33. the domain conferring the ability to direct virus-like particle assembly and release in SARS-CoV N is largely contained between residues 168 and 421 PMID: 18592403
    34. Phosphorylation of the arginine/serine motif of the nucleocapsid protein did not significantly affect RNA binding of the nucleocapsid protein but impaired its multimerization ability. PMID: 18631359
    35. This paper describes the work to identify two proteins/protein fragments of N protein and to determine their source. PMID: 18926799
    36. An excessive host immune response against the nucleocapsid protein of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) is involved in severe pneumonia caused by SARS-CoV infection. PMID: 18941225
    37. The SARS-CoV N protein is a modular protein containing multiple RNA-binding sites. PMID: 19052082
    38. thermostability of the N-terminal RNA-binding domain (RBD) of the N protein; results showed the thermal-induced unfolding-folding transition of the RBD follows a two-state model with a reversibility >90% PMID: 19254548
    39. SR-rich motif is critical for effective virus replication. PMID: 19370068
    40. SARS-CoV N protein specifically modulates transcription of the FGL2 gene to cause fibrosis and vascular thrombosis. PMID: 19423547

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  • 亞細胞定位：
    Virion. Host endoplasmic reticulum-Golgi intermediate compartment. Host Golgi apparatus. Host cytoplasm, host perinuclear region.
  • 蛋白家族：
    Betacoronavirus nucleocapsid protein family
  • 數(shù)據(jù)庫鏈接：

    KEGG: vg:1489678