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Recombinant Human Acylamino-acid-releasing enzyme (APEH)

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  • 中文名稱：
    Recombinant Human Acylamino-acid-releasing enzyme(APEH)
  • 貨號：
    CSB-EP001899HU
  • 規(guī)格：
    ￥1836
  • 圖片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

產(chǎn)品詳情

  • 純度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
    APEH
  • Uniprot No.：
  • 別名：
    AARE; ACPH; ACPH_HUMAN; Acyl peptide hydrolase; Acyl-peptide hydrolase; Acylamino acid releasing enzyme; Acylamino-acid-releasing enzyme; Acylaminoacyl peptidase; Acylaminoacyl-peptidase; APEH; APH; N acylaminoacyl peptide hydrolase; OPH; Oxidized protein hydrolase
  • 種屬：
    Homo sapiens (Human)
  • 蛋白長度：
    Full Length
  • 來源：
    E.coli
  • 分子量：
    85.2 kDa
  • 表達(dá)區(qū)域：
    1-732aa
  • 氨基酸序列
    MERQVLLSEPEEAAALYRGLSRQPALSAACLGPEVTTQYGGQYRTVHTEWTQRDLERMENIRFCRQYLVFHDGDSVVFAGPAGNSVETRGELLSRESPSGTMKAVLRKAGGTGPGEEKQFLEVWEKNRKLKSFNLSALEKHGPVYEDDCFGCLSWSHSETHLLYVAEKKRPKAESFFQTKALDVSASDDEIARLKKPDQAIKGDQFVFYEDWGENMVSKSIPVLCVLDVESGNISVLEGVPENVSPGQAFWAPGDAGVVFVGWWHEPFRLGIRFCTNRRSALYYVDLIGGKCELLSDDSLAVSSPRLSPDQCRIVYLQYPSLIPHHQCSQLCLYDWYTKVTSVVVDVVPRQLGENFSGIYCSLLPLGCWSADSQRVVFDSAQRSRQDLFAVDTQVGTVTSLTAGGSGGSWKLLTIDQDLMVAQFSTPSLPPTLKVGFLPSAGKEQSVLWVSLEEAEPIPDIHWGIRVLQPPPEQENVQYAGLDFEAILLQPGSPPDKTQVPMVVMPHGGPHSSFVTAWMLFPAMLCKMGFAVLLVNYRGSTGFGQDSILSLPGNVGHQDVKDVQFAVEQVLQEEHFDASHVALMGGSHGGFISCHLIGQYPETYRACVARNPVINIASMLGSTDIPDWCVVEAGFPFSSDCLPDLSVWAEMLDKSPIRYIPQVKTPLLLMLGQEDRRVPFKQGMEYYRALKTRNVPVRLLLYPKSTHALSEVEVESDSFMNAVLWLRTHLGS
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白標(biāo)簽：
    N-terminal 6xHis-tagged
  • 產(chǎn)品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    Tris-based buffer，50% glycerol
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    3-7 business days
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 產(chǎn)品描述：

    CUSABIO synthesized the recombinant gene by integrating the N-terminal 6xHis tag sequence into the targeted gene encoding the 1-732aa of the human APEH. The synthesized gene was subsequently cloned into an expression vector. After cloning, the expression vector was introduced into the E.coli for expression. The product was purified to obtain the recombinant human APEH protein carrying N-terminal 6xHis tag. The SDS-PAGE assayed the purity of this recombinant APEH protein greater than 85%. This APEH protein migrated along the gel to a band of about 90 kDa molecular weight.

    APEH is a gene providing instruction of making a protein called acylamino-acid-releasing enzyme in human and belongs to peptidase S9C family. This protein, also abbreviated APH or AARE, is a tetrameric serine protease which specifically removes acetyl amino acids from N-terminally acetylated peptides and play an important role in destroying oxidatively-damaged proteins in living cells. APH preferentially cleaves off Ac-Ala, Ac-Met and Ac-Ser. Deletions of this gene locus are found in various types of carcinomas, including small-cell lung carcinoma and renal cell carcinoma.

  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評價(jià)

問答及客戶評論

■ 常見問題解答
Q:

我們一直試圖使用AANA作為底物來驗(yàn)證其活性(在405 nm處產(chǎn)生并跟蹤硝基苯胺黃色產(chǎn)物),但沒有成功。我希望你能給我們一些建議。 我們將蛋白溶解在100ul去離子水中(1ug/uL)。我們在10 mM TRIS (pH = 8.0)和5 mM AANA(總體積200uL)中使用1、2和4ug蛋白質(zhì)，在37℃下孵育。我們沒有看到任何黃色產(chǎn)物，但我們的Hela細(xì)胞裂解物對照有。我們之前發(fā)布過這個(gè):https://www . science direct . com/science/article/pii/s 0009279721002775 如果你對我們?nèi)绾螠y試這種蛋白的活性有什么建議，請告訴我——我們想用它進(jìn)行動(dòng)力學(xué)分析。

A:
CSB-EP001899HU 該蛋白我們采用的是包涵體復(fù)性得到，雖然復(fù)性到了可溶性緩沖（不含有變性劑）但是也不能保證復(fù)性后的蛋白一定具有活性； 您可以嘗試下用APEH蛋白和LGALS8蛋白(CSB-EP012894HU) 進(jìn)行功能性Elisa驗(yàn)證，看下蛋白是否有活性。

靶點(diǎn)詳情

  • 功能：
    This enzyme catalyzes the hydrolysis of the N-terminal peptide bond of an N-acetylated peptide to generate an N-acetylated amino acid and a peptide with a free N-terminus. It preferentially cleaves off Ac-Ala, Ac-Met and Ac-Ser.
  • 基因功能參考文獻(xiàn)：
    1. Although APEH is primarily localized in the cytoplasm, a sub-fraction of this enzyme is sequestered at sites of nuclear damage following UVA irradiation or following oxidative stress. APEH interacts with the amino-terminal domain of XRCC1, and APEH facilitates both single-strand break repair and cell survival following exposure to H2O2. Thus, APEH as a novel proteolytic component of the DNA damage response. PMID: 28866241
    2. APEH polymorphism has significant influence on valproic acid pharmacokinetics in Chinese population PMID: 27406852
    3. Studies indicate that the cylindromatosis/turban tumor syndrome gene (CYLD) ranked highest, followed by acylaminoacyl-peptidase (APEH), dystroglycan (DAG1), macrophage-stimulating protein (MST1) and ubiquitin-specific peptidase 4 (USP4). PMID: 21931648
  • 亞細(xì)胞定位：
    Cytoplasm.
  • 蛋白家族：
    Peptidase S9C family
  • 數(shù)據(jù)庫鏈接：

    HGNC: 586

    OMIM: 102645

    KEGG: hsa:327

    STRING: 9606.ENSP00000296456

    UniGene: Hs.517969