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  4. Recombinant Chicken Vinculin (VCL), partial

Recombinant Chicken Vinculin (VCL), partial

  • 中文名稱：
    雞VCL重組蛋白
  • 貨號(hào)：
    CSB-YP025812CH
  • 規(guī)格：
  • 來(lái)源：
    Yeast
  • 其他：
  • 中文名稱：
    雞VCL重組蛋白
  • 貨號(hào)：
    CSB-EP025812CH
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 其他：
  • 中文名稱：
    雞VCL重組蛋白
  • 貨號(hào)：
    CSB-EP025812CH-B
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    雞VCL重組蛋白
  • 貨號(hào)：
    CSB-BP025812CH
  • 規(guī)格：
  • 來(lái)源：
    Baculovirus
  • 其他：
  • 中文名稱：
    雞VCL重組蛋白
  • 貨號(hào)：
    CSB-MP025812CH
  • 規(guī)格：
  • 來(lái)源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    VCL; VINC1; Vinculin; Metavinculin
  • 種屬：
    Gallus gallus (Chicken)
  • 蛋白長(zhǎng)度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion.
  • 基因功能參考文獻(xiàn)：
    1. VASP phosphorylation at Ser(157) mediates its localization at the membrane, but that VASP Ser(157) phosphorylation and membrane localization are not sufficient to activate its actin catalytic activity PMID: 25759389
    2. The importance of the actin-binding site in vinculin, which provide the molecular basis for elucidating additional roles of this interaction, including actin-induced conformational changes that promote actin bundling. PMID: 24685146
    3. Data show that alphaE-catenin D3 binds strongly to vinculin, whereas larger fragments and full-length alphaE-catenin bind approximately 1,000-fold more weakly. PMID: 22586082
    4. The vinculin C-terminal hairpin mediates F-actin bundle formation, focal adhesion, and cell mechanical properties. PMID: 22052910
    5. ACh stimulation regulates vinculin activation in tracheal smooth muscle via RhoA; vinculin activation facilitates connections between actin filaments and talin-integrin adhesion complexes and mediates the initiation of actin polymerization PMID: 21071443
    6. crystal structure: conformational changes in the head, tail and proline-rich domains are linked structurally and thermodynamically; and a combinatorial pathway to activation ensures that vinculin is activated only at sites of cell adhesion PMID: 15195105
    7. A double vinculin mutant protein Y100F/Y1065F localized to focal adhesion plaques. PMID: 15229287
    8. binding to F-actin potentiates a local rearrangement in the vinculin tail that in turn promotes vinculin dimerization and, hence, formation of actin bundles PMID: 16427016
    9. Results show that the interaction between two species with weak affinity in solution, such as vinculin and alpha-actinin, can be visualized in a membrane-like environment. PMID: 16430917
    10. role of pH on vinculin tail domain structure and self-association PMID: 18980387
    11. Lipid binding to the tail domain of vinculin: specificity and the role of the N and C termini PMID: 19110481
    12. vinculin can link adhesion plaques to the cytoskeleton by initiating the formation of bundled actin filaments or by remodeling existing filaments PMID: 19736312

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  • 亞細(xì)胞定位：
    Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction, adherens junction. Cell junction, focal adhesion. Cytoplasm, cytoskeleton. Cell membrane, sarcolemma; Peripheral membrane protein; Cytoplasmic side.
  • 蛋白家族：
    Vinculin/alpha-catenin family
  • 組織特異性：
    Isoform Metavinculin is muscle-specific.
  • 數(shù)據(jù)庫(kù)鏈接：