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  4. Phospho-SMAD5 (S463+S465) Recombinant Monoclonal Antibody

Phospho-SMAD5 (S463+S465) Recombinant Monoclonal Antibody

  • 中文名稱：
    磷酸化-SMAD5 (S463+S465)重組抗體
  • 貨號：
    CSB-RA859108A463phHU
  • 規(guī)格：
    ￥1320
  • 圖片：
    • IHC image of CSB-RA859108A463phHU diluted at 1:100 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：

    This phosphorylated SMAD5 antibody CSB-RA859108A463phHU is a recombinant monoclonal antibody produced from the expression of the plasmids that were integrated by the phospho-SMAD5 (S463+S465) monoclonal antibody DNA sequence in cell lines. The phospho-SMAD5 (S463+S465) monoclonal antibody was generated from splenocytes isolated from the animals that were immunized with the human phospho-SMAD5 (S463+S465). The phospho-SMAD5 (S463+S465) recombinant antibody is a rabbit IgG antibody. It underwent purification using the affinity-chromatography method. It can detect the human phospho-SMAD5 (S463+S465). And it is suitable for ELISA and IHC analyses.

    SMAD5 is a receptor-activated Smad that serves as an intracellular signal transducer for the transforming growth factor (TGF) superfamily. SMAD5 regulates cytoplasmic metabolic machinery and works as an intracellular pH messenger to maintain cell bioenergetic balance. SMAD5 is a negative regulator of embryonic hematopoiesis in a haploinsufficiency form, according to a recent study by Bing Liu et al., which helps to understand the cytogenetic mechanism by which SMAD5 serves as a leukemia suppressor.

  • Uniprot No.：
  • 基因名：
  • 別名：
    DKFZp781C1895 antibody; DKFZp781O1323 antibody; Dwfc antibody; hSmad5 antibody; JV5 1 antibody; JV5-1 antibody; MAD homolog 5 antibody; MAD, mothers against decapentaplegic homolog 5 antibody; MADH 5 antibody; MADH5 antibody; Mothers against decapentaplegic homolog 5 antibody; mothers against decapentaplegic, drosophila, homolog of, 5 antibody; Mothers against DPP homolog 5 antibody; MusMLP antibody; SMA and MAD related protein 5 antibody; SMAD 5 antibody; SMAD family member 5 antibody; SMAD, mothers against DPP homolog 5 antibody; Smad5 antibody; SMAD5_HUMAN antibody
  • 反應(yīng)種屬：
    Human
  • 免疫原：
    A synthesized peptide derived from Human Phospho-SMAD5 (S463+S465)
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    2G11
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, IHC
  • 推薦稀釋比：
    Application Recommended Dilution
    IHC 1:50-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能：
    Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD).
  • 基因功能參考文獻(xiàn)：
    1. We demonstrated high expression of miR-145 associated with late stage and unfavorable prognosis of esophageal cancer. We identified SMAD5 as direct target of miR-145, the suppressed expression of which consequently led to increased cell proliferation and migration/invasion. PMID: 29852786
    2. Smad5 acts as a intracellular pH messenger and maintains the bioenergetic homeostasis of cells by regulating cytoplasmic metabolic machinery. PMID: 28675158
    3. Data suggest that differences in expression levels in granulosa-like tumor cells and granulosa cells (GCs) from patients with polycystic ovary syndrome (PCOS) are due to increased expression of microRNA-27a-3p in GCs caused by insulin resistance in PCOS; microRNA-27a-3 expression is up-regulated in GCs in PCOS; overexpression of miR-27a-3p inhibits SMAD5 expression and promotes apoptosis. (SMAD5 = SMAD family member 5) PMID: 29029022
    4. Here the involvement of the pathway in adult brain function is suggested. This exploratory study establishes a strategy to better identify neuronal molecular signatures that are potentially associated with mental illness and cognitive deficits. We propose that the SMAD pathway may be a novel target in addressing cognitive deficit of SZ in future studies. PMID: 27727244
    5. miR-23a and miR-27a target SMAD5 and regulate apoptosis in human granulosa cells via the FasL-Fas pathway PMID: 26400397
    6. Our findings suggest that suppression of miR-222-3p activity promoted osteogenic differentiation hBMSCs through regulating Smad5-RUNX2 signaling axis. PMID: 26809090
    7. The polycomb group protein L3MBTL1 represses a SMAD5-mediated hematopoietic transcriptional program in human pluripotent stem cells. PMID: 25754204
    8. among the 15 SNPs, rs3206634 was significantly associated with KD in a recessive model (odds ratio = 2.31, p = 0.019), whereas there was no association between any of the 15 SNPs and CALs. PMID: 24163009
    9. Specific gene siRNAs knock-down further confirmed the osteogenic effects of Genistein on BMP2, SMAD5 and RUNX2 protein expression PMID: 24339730
    10. ATP production by NaF promotes hypertrophy-like changes via activation of phospho-Smad5 PMID: 23384547
    11. found that restoration of SMAD5, in addition to the TGF-beta type II receptor, which was epigenetically silenced by the latent viral protein latency-associated nuclear antigen, sensitized BC3 cells to the cytostatic effect of TGF-beta signaling PMID: 22013049
    12. Studies indicate SMAD5beta isoform plays role in hematopoietic stem cell homeostasis. PMID: 20148926
    13. regulatory signals are active at transcriptionally subnuclear sites PMID: 12060751
    14. Smad5 activation by BMP4 in human hematopoietic cells results in significantly increased proliferation of erythroid progenitors and formation of glycophorin-A+ cells. PMID: 12064918
    15. up-regulated Smad5 mediates apoptosis of gastric epithelial cells induced by Helicobacter pylori infection PMID: 12473652
    16. Results demonstrate a mitochondrial distribution of Smad5 in non-stimulated chondroprogenitor cells. PMID: 12849988
    17. SMAD5 undergoes copy number gain and increased expression, rather than loss of expression, and therefore does not act as a tumor-suppressor gene in hepatocellular carcinoma PMID: 14670176
    18. In mature human B cells, BMP-6 inhibited cell growth, and rapidly induced phosphorylation of Smad5. PMID: 15877825
    19. We used homology-modeling techniques to generate a reliable molecular model of the Smad5 MH1 domain based on the crystal structure of Smad3 MH1 domain PMID: 16243555
    20. SMAD5 gene was found to be associated with schizophrenia. PMID: 18298822

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  • 亞細(xì)胞定位：
    Cytoplasm. Nucleus. Note=Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4.
  • 蛋白家族：
    Dwarfin/SMAD family
  • 組織特異性：
    Ubiquitous.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 6771

    OMIM: 603110

    KEGG: hsa:4090

    STRING: 9606.ENSP00000441954

    UniGene: Hs.167700