NANS Antibody
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中文名稱(chēng):NANS兔多克隆抗體
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貨號(hào):CSB-PA885699LA01HU
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規(guī)格:¥440
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促銷(xiāo):
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圖片:
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Western Blot
Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate
All lanes: NANS antibody at 5.5μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 41 kDa
Observed band size: 41 kDa -
Immunofluorescence staining of HepG2 cells with CSB-PA885699LA01HU at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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IHC image of CSB-PA885699LA01HU diluted at 1:200 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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IHC image of CSB-PA885699LA01HU diluted at 1:200 and staining in paraffin-embedded human gastric cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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其他:
產(chǎn)品詳情
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產(chǎn)品名稱(chēng):Rabbit anti-Homo sapiens (Human) NANS Polyclonal antibody
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Uniprot No.:
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基因名:NANS
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別名:EC 2.5.1.56 antibody; EC 2.5.1.57 antibody; N acetylneuraminate 9 phosphate synthase antibody; N acetylneuraminate synthase antibody; N acetylneuraminic acid phosphate synthase antibody; N acetylneuraminic acid synthase antibody; N-acetylneuraminate synthase antibody; N-acetylneuraminate-9-phosphate synthase antibody; N-acetylneuraminic acid phosphate synthase antibody; N-acetylneuraminic acid synthase antibody; NANS antibody; OTTHUMP00000021769 antibody; SAS antibody; Sialic acid phosphate synthase antibody; Sialic acid synthase antibody; SIAS_HUMAN antibody
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宿主:Rabbit
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反應(yīng)種屬:Human
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免疫原:Recombinant Human Sialic acid synthase protein (132-259AA)
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免疫原種屬:Homo sapiens (Human)
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標(biāo)記方式:Non-conjugated
本頁(yè)面中的產(chǎn)品,NANS Antibody (CSB-PA885699LA01HU),的標(biāo)記方式是Non-conjugated。對(duì)于NANS Antibody,我們還提供其他標(biāo)記。見(jiàn)下表:
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克隆類(lèi)型:Polyclonal
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抗體亞型:IgG
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純化方式:>95%, Protein G purified
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濃度:It differs from different batches. Please contact us to confirm it.
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保存緩沖液:Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 -
產(chǎn)品提供形式:Liquid
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應(yīng)用范圍:ELISA, WB, IHC, IF
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推薦稀釋比:
Application Recommended Dilution WB 1:500-1:5000 IHC 1:200-1:500 IF 1:50-1:200 -
Protocols:
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儲(chǔ)存條件:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期:Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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功能:Produces N-acetylneuraminic acid (Neu5Ac) and 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (KDN). Can also use N-acetylmannosamine 6-phosphate and mannose 6-phosphate as substrates to generate phosphorylated forms of Neu5Ac and KDN, respectively.
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基因功能參考文獻(xiàn):
- NANS deficiency is associated with defect in brain and skeletal development. PMID: 27213289
- sialic acid synthases kinetics and preliminary mutagenesis experiments reveal the importance of C-terminal antifreeze protein domain PMID: 16274664
- The structure of the C-terminal antifreeze-like domain of human sialic acid synthase was determined. The structure comprises one alpha- and two single-turn 3(10)-helices and two beta-strands, and is similar to those of the type III antifreeze proteins. PMID: 16597820
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相關(guān)疾?。?/div>Spondyloepimetaphyseal dysplasia, Genevieve type (SEMDG)組織特異性:Ubiquitous.數(shù)據(jù)庫(kù)鏈接:
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