FICD Antibody
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中文名稱(chēng):FICD兔多克隆抗體
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貨號(hào):CSB-PA880112LA01HU
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規(guī)格:¥440
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促銷(xiāo):
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圖片:
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Western Blot
Positive WB detected in: A549 whole cell lysate, Hela whole cell lysate, Rat kidney tissue
All lanes: FICD antibody at 3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa -
IHC image of CSB-PA880112LA01HU diluted at 1:500 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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IHC image of CSB-PA880112LA01HU diluted at 1:500 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Immunofluorescence staining of PC-3 cells with CSB-PA880112LA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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其他:
產(chǎn)品詳情
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產(chǎn)品名稱(chēng):Rabbit anti-Homo sapiens (Human) FICD Polyclonal antibody
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Uniprot No.:
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基因名:FICD
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別名:FICD; HIP13; HYPE; UNQ3041/PRO9857; Protein adenylyltransferase FICD; AMPylator FICD; De-AMPylase FICD; FIC domain-containing protein; Huntingtin yeast partner E; Huntingtin-interacting protein 13; HIP-13; Huntingtin-interacting protein E
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宿主:Rabbit
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反應(yīng)種屬:Human, Rat
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免疫原:Recombinant Human Adenosine monophosphate-protein transferase FICD protein (63-185AA)
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免疫原種屬:Homo sapiens (Human)
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標(biāo)記方式:Non-conjugated
本頁(yè)面中的產(chǎn)品,F(xiàn)ICD Antibody (CSB-PA880112LA01HU),的標(biāo)記方式是Non-conjugated。對(duì)于FICD Antibody,我們還提供其他標(biāo)記。見(jiàn)下表:
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克隆類(lèi)型:Polyclonal
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抗體亞型:IgG
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純化方式:>95%, Protein G purified
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濃度:It differs from different batches. Please contact us to confirm it.
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保存緩沖液:Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 -
產(chǎn)品提供形式:Liquid
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應(yīng)用范圍:ELISA, WB, IHC, IF
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推薦稀釋比:
Application Recommended Dilution WB 1:500-1:5000 IHC 1:500-1:1000 IF 1:50-1:200 -
Protocols:
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儲(chǔ)存條件:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期:Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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功能:Protein that can both mediate the addition of adenosine 5'-monophosphate (AMP) to specific residues of target proteins (AMPylation), and the removal of the same modification from target proteins (de-AMPylation), depending on the context. The side chain of Glu-231 determines which of the two opposing activities (AMPylase or de-AMPylase) will take place. Acts as a key regulator of the ERN1/IRE1-mediated unfolded protein response (UPR) by mediating AMPylation or de-AMPylation of HSPA5/BiP. In unstressed cells, acts as an adenylyltransferase by mediating AMPylation of HSPA5/BiP at 'Thr-518', thereby inactivating it. In response to endoplasmic reticulum stress, acts as a phosphodiesterase by mediating removal of ATP (de-AMPylation) from HSPA5/BiP at 'Thr-518', leading to restore HSPA5/BiP activity. Although it is able to AMPylate RhoA, Rac and Cdc42 Rho GTPases in vitro, Rho GTPases do not constitute physiological substrates.
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基因功能參考文獻(xiàn):
- The single catalytic domain of FICD can release the attached AMP, restoring functionality to BiP. PMID: 27918543
- We first demonstrate efficient enrichment and fast visualization of potential HYPE substrates in cell lysates by in-gel fluorescence, followed by robust identification via shotgun proteomics on a QExactive mass spectrometer PMID: 26604261
- HYPE is an unfolded protein response regulator. PMID: 25601083
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亞細(xì)胞定位:Endoplasmic reticulum membrane; Single-pass type II membrane protein.
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蛋白家族:Fic family
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組織特異性:Ubiquitous.
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數(shù)據(jù)庫(kù)鏈接:
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