ELP3 Antibody
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中文名稱:ELP3兔多克隆抗體
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貨號(hào):CSB-PA884499LA01HU
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規(guī)格:¥440
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促銷:
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圖片:
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Western Blot
Positive WB detected in: Jurkat whole cell lysate, K562 whole cell lysate, 293 whole cell lysate
All lanes: ELP3 antibody at 3.82µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 63, 61, 50, 53 kDa
Observed band size: 63 kDa -
IHC image of CSB-PA884499LA01HU diluted at 1:400 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Immunofluorescence staining of Hela cells with CSB-PA884499LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Immunoprecipitating ELP3 in HEK293 whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA884499LA01HU in HEK293 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA884499LA01HU (6µg) + HEK293 whole cell lysate (500µg)
Lane 3: HEK293 whole cell lysate (20µg)
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其他:
產(chǎn)品詳情
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產(chǎn)品名稱:Rabbit anti-Homo sapiens (Human) ELP3 Polyclonal antibody
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Uniprot No.:
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基因名:ELP3
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別名:DKFZp761L098 antibody; Elongation protein 3 homolog (S. cerevisiae) antibody; Elongation protein 3 homolog antibody; ELONGATION PROTEIN 3; S. CEREVISIAE; HOMOLOG OF antibody; elongator acetyltransferase complex subunit 3 antibody; Elongator complex protein 3 antibody; elp3 antibody; ELP3_HUMAN antibody; FLJ10422 antibody; hELP3 antibody; Hypothetical protein DKFZp761L098 antibody; Kat9 antibody
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宿主:Rabbit
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反應(yīng)種屬:Human
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免疫原:Recombinant Human Elongator complex protein 3 protein (1-85AA)
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免疫原種屬:Homo sapiens (Human)
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標(biāo)記方式:Non-conjugated
本頁面中的產(chǎn)品,ELP3 Antibody (CSB-PA884499LA01HU),的標(biāo)記方式是Non-conjugated。對(duì)于ELP3 Antibody,我們還提供其他標(biāo)記。見下表:
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克隆類型:Polyclonal
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抗體亞型:IgG
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純化方式:>95%, Protein G purified
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濃度:It differs from different batches. Please contact us to confirm it.
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保存緩沖液:Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 -
產(chǎn)品提供形式:Liquid
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應(yīng)用范圍:ELISA, WB, IHC, IF, IP
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推薦稀釋比:
Application Recommended Dilution WB 1:500-1:5000 IHC 1:200-1:500 IF 1:50-1:200 IP 1:200-1:2000 -
Protocols:
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儲(chǔ)存條件:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期:Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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功能:Catalytic tRNA acetyltransferase subunit of the RNA polymerase II elongator complex, which is a component of the RNA polymerase II (Pol II) holoenzyme and is involved in transcriptional elongation. The elongator complex is required for multiple tRNA modifications, including mcm5U (5-methoxycarbonylmethyl uridine), mcm5s2U (5-methoxycarbonylmethyl-2-thiouridine), and ncm5U (5-carbamoylmethyl uridine). In the elongator complex, acts as a tRNA uridine(34) acetyltransferase by mediating formation of carboxymethyluridine in the wobble base at position 34 in tRNAs. May also act as a protein lysine acetyltransferase by mediating acetylation of target proteins; such activity is however unclear in vivo and recent evidences suggest that ELP3 primarily acts as a tRNA acetyltransferase. Involved in neurogenesis: regulates the migration and branching of projection neurons in the developing cerebral cortex, through a process depending on alpha-tubulin acetylation. Required for acetylation of GJA1 in the developing cerebral cortex.
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基因功能參考文獻(xiàn):
- and CTU1/2, partner enzymes in U34 methoxycarbonylmethyl-2-thio tRNA modification, are up-regulated in human breast cancers and sustain metastasis. PMID: 27811057
- Promoter hypermethylation is an important mechanism of the transcriptional inactivation of ELP3 in invasive ductal breast carcinoma. PMID: 25148870
- Regulation of G6PD acetylation by SIRT2 and KAT9 modulates NADPH homeostasis and cell survival during oxidative stress. PMID: 24769394
- data suggest that hElp3 can regulate the transcription of HSP70 gene, and the HAT domain of hElp3 is essential for this function PMID: 22216241
- The results of this study uncover a novel role for Elp3 in the regulation of synaptic bouton expansion during neurogenesis that may be linked with a requirement for sleep. PMID: 20626565
- Elp3 promotes the acetylation of alpha-tubulin in microtubules in neurological disorders. PMID: 20036197
- We used hELP3 antisense oligonucl. to knock down hELP3 gene. The results showed that reduction of hELP3 mRNA and protein caused a suppression of HSP70-2 and histone H3 hypoacetylation. PMID: 17558451
- Allelic variants of ELP3 were associated with amyotrophic lateral sclerosis in three populations comprising 1483 people. PMID: 18996918
- ELP3 localises to mitochondria in HeLa cells, actin-like filaments, and actin-rich sites at the edges of spreading cells. This suggests that ELP3 and the ELONGATOR complex may play a role in mitochondrial function, actin organisation, and cell motility. PMID: 19429107
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相關(guān)疾病:ELP3 genetic variations may be associated with an increased risk for neurodegeneration and motor neuron diseases.
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亞細(xì)胞定位:Cytoplasm. Nucleus.; [Isoform 1]: Nucleus.; [Isoform 2]: Cytoplasm. Nucleus.
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蛋白家族:ELP3 family
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組織特異性:Expressed in the cerebellum and spinal motor neurons.
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數(shù)據(jù)庫鏈接:
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