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Cleaved-NOTCH2 (A1734) Antibody

  • 中文名稱：
    Cleaved-NOTCH2 (A1734)兔多克隆抗體
  • 貨號：
    CSB-PA020143
  • 規(guī)格：
    ￥880
  • 圖片：
    • Western Blot analysis of Jurkat cells using Cleaved-Notch 2 (A1734) Polyclonal Antibody
  • 其他：

產(chǎn)品詳情

  • Uniprot No.：
  • 基因名：
  • 別名：
    AGS2 antibody; hN2 antibody; Motch B antibody; N2 antibody; N2ECD antibody; N2ICD antibody; neurogenic locus notch homolog protein 2 antibody; NOTC2_HUMAN antibody; Notch 2 antibody; Notch 2 intracellular domain antibody; Notch Drosophila homolog 2 antibody; Notch homolog 2 antibody; Notch homolog 2 Drosophila antibody; Notch2 antibody
  • 宿主：
    Rabbit
  • 反應(yīng)種屬：
    Human,Mouse,Rat
  • 免疫原：
    Synthesized peptide derived from the Internal region of Human Notch 2.
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated
  • 抗體亞型：
    IgG
  • 純化方式：
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    WB, ELISA
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:2000
    ELISA 1:10000
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

產(chǎn)品評價

靶點(diǎn)詳情

  • 功能：
    Functions as a receptor for membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1) to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. Involved in bone remodeling and homeostasis. In collaboration with RELA/p65 enhances NFATc1 promoter activity and positively regulates RANKL-induced osteoclast differentiation. Positively regulates self-renewal of liver cancer cells.
  • 基因功能參考文獻(xiàn)：
    1. TNFalpha regulates NOTCH2 and NOTCH3 expression in pulmonary artery smooth muscle cells via preferential ACTR-IIA signalling in BMPR-II-deficient cells. PMID: 28084316
    2. results confirm the association of the NOTCH2-mutation with shorter median treatment-free survival and suggest the possible usefulness of the identification of these changes for the diagnosis of splenic marginal zone lymphoma. PMID: 28522570
    3. BANCR may promote melanoma cell growth through inhibition of miR204, leading to the activation of Notch2 pathway. Authors further demonstrated that BANCR knockdown inhibited tumor growth in vivo. Results suggest the BANCR/miR204/Notch2 axis mediates melanoma cell proliferation and tumor progression. PMID: 29075789
    4. Altered expression of WFS1 and NOTCH2 genes may play a role in pathogenesis and development of DN in patients with T2DM. PMID: 29626590
    5. Notch2 is important in Club cell differentiation in normal lungs and adenocarcinoma. Notch2 is regulated mutually with Notch1, and the balance of the expression of Notch receptors could determine the biological behaviours of lung cancer cells. PMID: 28666642
    6. Notch2 is up-regulated in oesophageal squamous cell carcinoma tissues and could serve as a promising biomarker for identifying individuals with poor prognostic potential. PMID: 27158037
    7. The SNHG12/miR-195-5p/Notch2-Notch signaling pathway axis might become a novel therapeutic target for osteosarcoma. SNHG12 functioned as a competing endogenous RNA, modulating the expression of Notch2 by sponging miR-195-5p in osteosarcoma. PMID: 29229388
    8. NOTCH2 acts as an oncogene that promotes bladder cancer growth and metastasis through epithelial-to-mesenchymal transition, cell-cycle progression, and maintenance of stemness. Inhibition of NOTCH2 is a rational novel treatment strategy for invasive bladder cancer. PMID: 26769750
    9. Examination of the molecular underpinnings of this "NOTCH2-BCR axis" in cGVHD revealed imbalanced expression of the transcription factors IRF4 and IRF8, each critical to B-cell differentiation and fate. All-trans retinoic acid (ATRA) increased IRF4 expression, restored the IRF4-to-IRF8 ratio, abrogated BCR-NOTCH hyperactivation, and reduced NOTCH2 expression in cGVHD B cells without compromising viability. PMID: 28851699
    10. Genetic variation in NOTCH2 was associated with troponin T levels in women with psychosis. PMID: 28167435
    11. Human biliary atresia and 3,5-diethoxycarbonyl-1,4-dihydrocollidine (DDC)-induced experimental cholestasis in mice are associated with increased expression of Notch2. PMID: 28688656
    12. The data showed that the overexpression of miR-18a-5p could downregulate Notch2 expression and subsequently suppress endothelial-mesenchymal transition and cardiac fibrosis. PMID: 28733035
    13. Mutation in NOTCH2 gene is associated with nodal marginal zone lymphoma. PMID: 27335277
    14. Transgenic mice harboring Notch2 mutation analogous to that in patients with Hajdu-Cheney syndrome (HCS) are severely osteopenic because of enhanced bone resorption; this model has now been validated. Data from further studies in transgenic mice suggest that the HCS mutation in osteoblasts, but not in osteoclasts, causes osteopenia in this model. PMID: 28592489
    15. Intermittent compressive stress regulates Notch receptor and target gene expression via the TGF-beta signaling pathway. Notch signaling participates in TGF-beta-induced sclerostin expression in periodontal ligament cells. PMID: 27966788
    16. The authors present novel structures of human ligands Jagged2 and Delta-like4 and human Notch2, together with functional assays, which suggest that ligand-mediated coupling of membrane recognition and Notch binding is likely to be critical in establishing the optimal context for Notch signalling. PMID: 28572448
    17. our results indicated epidermal growth factor-like domain multiple 7 protein participates in growth hormone-secreting pituitary adenoma proliferation and invasion regulation via Notch2/DLL3 signaling pathway. These findings raised the possibility that epidermal growth factor-like domain multiple 7 protein might serve as a useful biomarker to assess growth hormone-secreting pituitary adenoma invasion and prognosis PMID: 28705113
    18. Mutations in NOTCH2 gene is associated with T-cell acute lymphoblastic leukemia. PMID: 27717083
    19. Together, these data show that miR-181a may play an essential role in GSC formation and GBM progression by targeting Notch2, suggesting that Notch2 and miR-181a have potential prognostic value as tumor biomarkers in GBM patients. PMID: 28389242
    20. MicroRNA-146a may increase the IL-6 levels and exacerbate Graves Ophthalmopathy by directly targeting Notch2. PMID: 28278511
    21. High NOTCH2 expression is associated with metastasis in colorectal cancer. PMID: 28161537
    22. this study shows that Treg cells infiltrating uveitic eyes display elevated Notch2 expression PMID: 27564686
    23. miR-146a-5p functions as a tumour-suppressive miRNA targeting Notch2 and inhibits the EMT progression of ESCC PMID: 27832663
    24. Notch2 may confer stemness properties in HCC. PMID: 27221981
    25. AGS is caused by mutations in one of two genes, namely, JAG1 or NOTCH2. These genes are part of the Notch signaling pathway, which is involved in cell fate determination. JAG1 mutations have been identified in 70-94% of individuals with clinically diagnosed AGS PMID: 25676721
    26. This is supported by the depletion of CTCF in glioblastoma cells affecting the expression levels of NOTCH2 as a target of miR-181c. CONCLUSION: Together, our results point to the epigenetic role of CTCF in the regulation of microRNAs implicated in tumorigenesis PMID: 26983574
    27. suggest that Notch2 has an essential role in the cell growth, invasion, and migration of SACC. Notch2 may therefore be a potential target gene for the treatment of SACC by interfering with cell growth and metastasis PMID: 26427670
    28. Notch2 and Notch3 inhibited both cell proliferation and cell apoptosis in BeWo and JAR trophoblast cell lines. PMID: 26640406
    29. Results suggest that Notch2 pathway and miR-23b interplay in a reciprocal regulation loop in gastric cancer cells and this axis plays an important role in gastric carcinogenesis. PMID: 26041881
    30. These findings suggested that the NOTCH2 signaling may confer aggressive behavior and immature morphology in human hepatocellular carcinoma cells. PMID: 26252838
    31. High Notch2 expression is associated with chronic myeloid leukemia. PMID: 25849484
    32. miR-191 represses proliferation in primary human fibroblasts via targeting multiple proto-oncogenes, including CDK9, NOTCH2, and RPS6KA3. PMID: 25992613
    33. C8orf4 negatively regulates the self-renewal of liver CSCs via suppression of NOTCH2 signalling PMID: 25985737
    34. NOTCH2 inhibition triggers the Epstein-Barr virus lytic cycle and cell apoptosis; and NOTCH2 inhibition may represent an appealing therapeutic strategy against Epstein-Barr virus-associated malignancies. PMID: 26018735
    35. Notch2 controls prolactin and insulin-like growth factor binding protein-1 expression in decidualizing human stromal cells of early pregnancy. PMID: 25397403
    36. Hajdu-Cheney syndrome and serpentine fibula-polycystic kidney syndrome are a single disease entity with a wide spectrum of clinical manifestations associated with truncating mutations in exon 34 of NOTCH2. PMID: 25696021
    37. Data suggest that expression of NOTCH2 in first-trimester placenta is cell-type specific; NOTCH2 is expressed in differentiated cells of extravillous trophoblast lineage; inhibition of NOTCH2 by RNA interference promotes trophoblast motility. PMID: 25659500
    38. a novel biological method that entails selection of human BMSCs based on NOTCH2 expression and activation of the NOTCH signaling pathway in cultured BMSCs via a tissue culture plate coated with recombinant human JAGGED1 (JAG1) ligand. PMID: 25368376
    39. This review establishes that gain-of-function mutations of NOTCH2 are associate with Hajdu-Cheney syndrome. PMID: 25491639
    40. NOTCH2 mutations were associated with diffuse large B-cell lymphoma with hepatitis C virus infection. PMID: 25381127
    41. NOTCH2 inhibits PDGF-B-dependent proliferation and its expression is decreased by PDGF-B. PMID: 25957400
    42. Inhibition of Notch2 prevents goblet cell metaplasia induced by a broad range of stimuli. PMID: 25558064
    43. Human NOTCH2 but not mouse Notch2 is resistant to negative regulatory region perturbation and ligand-independent activation by Adam17. PMID: 25918160
    44. drives multiple myeloma associated osteoclast development and bone destruction PMID: 25257302
    45. High NOTCH2 expression is associated with minimal deviation adenocarcinoma of uterine cervix. PMID: 25381598
    46. 28 of 30 in: Mol Med Rep. 2015 Jan;11(1) Interference of Notch 2 inhibits the progression of gliomas and induces cell apoptosis by induction of the cell cycle at the G0/G1 phase PMID: 25338527
    47. The Notch2 receptor with PEST domain truncation enhances cell proliferation which may be associated with the activation of the Notch2 and the NF-kappaB signaling. PMID: 25314575
    48. The cumulative survival rate was significantly longer in the Notch2shRNA group. PMID: 25323114
    49. All three syndromes result from mutations in the gene that encodes NOTCH2. PMID: 24995648
    50. In the placentas from women with early-onset severe preeclampsia, Notch2 expression was significantly increased. PMID: 24336671

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  • 相關(guān)疾?。?/div>
    Alagille syndrome 2 (ALGS2); Hajdu-Cheney syndrome (HJCYS)
  • 亞細(xì)胞定位：
    [Notch 2 extracellular truncation]: Cell membrane; Single-pass type I membrane protein.; [Notch 2 intracellular domain]: Nucleus. Cytoplasm.
  • 蛋白家族：
    NOTCH family
  • 組織特異性：
    Expressed in the brain, heart, kidney, lung, skeletal muscle and liver. Ubiquitously expressed in the embryo.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 7882

    OMIM: 102500

    KEGG: hsa:4853

    STRING: 9606.ENSP00000256646

    UniGene: Hs.487360