BTN3A1 Antibody
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中文名稱:BTN3A1兔多克隆抗體
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貨號:CSB-PA002873LA01HU
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規(guī)格:¥3900
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圖片:
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Western Blot
Positive WB detected in: U937 whole cell lysate, K562 whole cell lysate, 293 whole cell lysate, A549 whole cell lysate
All lanes: BTN3A1 antibody at 1: 1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 40-58 kDa
Observed band size: 48 kDa -
Immunofluorescence staining of Hela cell with CSB-PA002873LA01HU at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Immunofluorescence staining of SH-SY5Y cell with CSB-PA002873LA01HU at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Immunofluorescence staining of SH-SY5Y cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:
To generate the BTN3A1 polyclonal antibody, rabbits were immunized with recombinant human BTN3A1 (30-254aa). The antibody was then purified using Protein G to achieve a purity level of over 95%, ensuring its high quality and freedom from impurities that may affect experimental outcomes. This antibody is capable of detecting the BTN3A1 protein in both human and mouse samples, making it a useful tool for research applications.
The BTN3A1 polyclonal antibody has been tested in a range of applications, including ELISA, WB, and IHC, enabling protein detection, determination of presence and size, and localization of BTN3A1 protein.
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產(chǎn)品名稱:Rabbit anti-Homo sapiens (Human) BTN3A1 Polyclonal antibody
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Uniprot No.:
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基因名:BTN3A1
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別名:BT3A1_HUMAN antibody; BTN3A1 antibody; Butyrophilin subfamily 3 member A1 antibody; CD277 antibody
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宿主:Rabbit
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反應(yīng)種屬:Human
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免疫原:Recombinant Human Butyrophilin subfamily 3 member A1 protein
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免疫原種屬:Homo sapiens (Human)
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標(biāo)記方式:Non-conjugated
本頁面中的產(chǎn)品,BTN3A1 Antibody (CSB-PA002873LA01HU),的標(biāo)記方式是Non-conjugated。對于BTN3A1 Antibody,我們還提供其他標(biāo)記。見下表:
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克隆類型:Polyclonal
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抗體亞型:IgG
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純化方式:Antigen?affinity?purification
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濃度:It differs from different batches. Please contact us to confirm it.
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保存緩沖液:Preservative:?0.02%?sodium?azide
Constituents:?PBS?containing?50%?glycerol pH 7.3 -
產(chǎn)品提供形式:Liquid
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應(yīng)用范圍:ELISA,WB?,IF
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推薦稀釋比:
Application Recommended Dilution Recommended ?Recommended?dilution:?WB:1:500-1:1000? IF ?1:20-1:200 -
Protocols:
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儲存條件:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期:Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
相關(guān)產(chǎn)品
靶點詳情
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功能:Plays a role in T-cell activation and in the adaptive immune response. Regulates the proliferation of activated T-cells. Regulates the release of cytokines and IFNG by activated T-cells. Mediates the response of T-cells toward infected and transformed cells that are characterized by high levels of phosphorylated metabolites, such as isopentenyl pyrophosphate.
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基因功能參考文獻(xiàn):
- Microtubule-associated protein 4 (MAP4) controls the dynein-dependent transport of BTN3A1 in response to nucleic acid stimulation, thereby identifying MAP4 as an upstream regulator of BTN3A1. Thus, the depletion of either MAP4 or BTN3A1 impairs cytosolic DNA- or RNA-mediated type I IFN responses. PMID: 27911820
- results show that ligand binding to BTN3A1 induces a conformational change within the intracellular domain that involves the JM region and is required for full activation. PMID: 28705810
- findings show that changes in the juxtamembrane domain of BTN3A1, but not its transmembrane domain, induce a markedly enhanced or reduced gammadelta T cell reactivity PMID: 28461569
- These findings support intracellular sensing of prenyl pyrophosphates by BTN3A1 rather than extracellular presentation. PMID: 26475929
- Human gamma-delta T cells are activated by cytosolic interactions of BTN3A1 with soluble phosphoantigens and the cytoskeletal adaptor periplakin. PMID: 25637025
- evidence that gene(s) on Chr6 in addition to BTN3A1 are mandatory for PAg-mediated activation of Vgamma9Vdelta2 T cells. PMID: 24890657
- Ligand binding to the BTN3A1 B30.2 domain affects residues in the juxtamembrane region, suggesting ligand-induced conformational change. PMID: 25065532
- These studies demonstrate that internal sensing of changes in pAg metabolite concentrations by BTN3A1 molecules is a critical step in Vgamma9Vdelta2 T cell detection of infection and tumorigenesis. PMID: 24703779
- BTN3A1 represents an antigen-presenting molecule required for the activation of Vgamma9Vdelta2 T cells. PMID: 23872678
- investigation of role of CD277 in activation/inactivation of T-lymphocytes: Data indicate that modulation of CD277 interaction (with agonists or blocking antibodies) with T-cell antigen receptor can modulate activation/inactivation of T-lymphocytes. PMID: 22767497
- BTN3A1 is necessary for Vgamma9Vdelta2 activation and begin to unravel the extracellular events that occur during stimulation through the Vgamma9Vdelta2 T cell receptor. PMID: 22846996
- CD277 triggering is not involved in CD16- or NKp46-induced NK cell activation. PMID: 21918970
- Results point to a role for CD277 up-regulated by microenvironmental signals in the acquisition of a regulatory phenotype by ovarian tumor-associated myeloid cells. PMID: 21113407
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亞細(xì)胞定位:Cell membrane; Single-pass type I membrane protein.
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蛋白家族:Immunoglobulin superfamily, BTN/MOG family
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組織特異性:Detected on T-cells, natural killer cells, dendritic cells and macrophages (at protein level). Ubiquitous. Highly expressed in heart, pancreas and lung, Moderately expressed in placenta, liver and muscle.
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