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Recombinant Dog Angiopoietin-2 (ANGPT2) (Active)

In Stock
  • 中文名稱：
  • 貨號(hào)：
    CSB-MP001707DO
  • 規(guī)格：
    ￥1908
  • 圖片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
    • Activity
      Measured by its binding ability in a functional ELISA. Immobilized Dog ANGPT2 at 2 μg/ml can bind anti-ANGPT2 recombinant antibody (CSB-RA001707MA01HU), the EC50 is 2.836-4.992 ng/mL. Biological Activity Assay
  • 其他：

產(chǎn)品詳情

  • 純度：
    Greater than 95% as determined by SDS-PAGE.
  • 內(nèi)毒素：
    Less than 1.0 EU/ug as determined by LAL method.
  • 生物活性：
    Measured by its binding ability in a functional ELISA. Immobilized Dog ANGPT2 at 2 μg/mL can bind anti-ANGPT2 recombinant antibody (CSB-RA001707MA01HU), the EC50 is 2.836-4.992 ng/mL.
  • 基因名：
  • Uniprot No.：
  • 別名：
    (ANG-2)
  • 分子結(jié)構(gòu)：
  • 種屬：
    Canis lupus familiaris (Dog) (Canis familiaris)
  • 蛋白長度：
    Full Length of Mature Protein
  • 來源：
    Mammalian cell
  • 分子量：
    57.2 kDa
  • 表達(dá)區(qū)域：
    19-495aa
  • 氨基酸序列
    YNNFRRSMDSIGRRQYQVQHGSCSYTFLLPETDNCRSPGSYVPNAVQRDAPLDYDDSVQRLQVLENIMENNTQWLIKLENYIQDNMKKEMVEMQQNAVQNQTAVMIEIGTNLLNQTAEQTRKLTDVEAQVLNQTTRLELQLLEHSLSTNKLEKQILDQTSEINKLQDKNSFLEKKVLDMEDKHIVQLRSIKEEKDQLQVLVSKQNSIIEELEKQLVTATVNNSVLQKQQHDLMETVHSLLTMISPSKSPKDTFVAKEEQIIYRDCAEVFKSGLTTNGIYTLTFPNSTEEIKAYCDMETSGGGWTVIQRREDGSVDFQRTWKEYKVGFGNPSGEHWLGNEFVFQVTNQQPYVLKIHLKDWEGNEAYSLYEHFYLSGEELNYRIHLKGLTGTAGKISSISQPGNDFSTKDADNDKCICKCSQMLTGGWWFDACGPSNLNGMYYPQRQNTNKFNGIKWYYWKGSGYSLKGTTMMIRPADF
  • 蛋白標(biāo)簽：
    C-terminal 6xHis-tagged
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 產(chǎn)品描述：

    This Recombinant Dog Angiopoietin-2 (ANGPT2) was produced in Mammalian cell, where the gene sequence encoding Dog ANGPT2 (19-495aa) was expressed with the C-terminal 6xHis tag. The purity of this ANGPT2 protein was greater than 95% by SDS-PAGE. The activity was validated by its binding ability in a functional ELISA.

    The similarity between dog ANGPT2 and human ANGPT2 is very high, reaching 92.13%. The dog ANGPT2 gene encodes 495 amino acids, the first 18 amino acids are signal peptides, and the mature protein has 477 amino acids. ANGPT2 is mainly secreted by endothelial cells. It is a natural competitive antagonist of ANGPT1. It can competitively inhibit the phosphorylation of ANGPT1 to promote TIE2. ANGPT2 is mainly expressed in vascular endothelial cells and can maintain the dynamic of vascular growth and degeneration by inhibiting the activation of ANGPT1 balance. In addition, the expression of ANGPT2 is regulated by a variety of factors such as VEGF, EGF, bFGF, TNFα, etc. Hypoxia can promote the expression of ANGPT2, However, ANGPT1 and TGF-β down-regulated their expression levels. The effect of ANGPT2 on angiogenesis is related to the local microenvironment. In the presence of VEGF, ANGPT2 can antagonize the effect of ANGPT1 on vascular structure stabilization, which is beneficial to vascular sprouting and growth. When VEGF is lacking, ANGPT2 inhibits ANGPT1, which is beneficial to vascular regression.

  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating angiogenic signals mediated by ANGPT1. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal.
  • 亞細(xì)胞定位：
    Secreted.
  • 數(shù)據(jù)庫鏈接：