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  4. Sheep Interferon γ,IFN-γ ELISA Kit

Sheep Interferon γ,IFN-γ ELISA Kit

  • 中文名稱：
    綿羊γ干擾素(IFN-γ)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E14018Sh
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：

    This Sheep IFNG ELISA Kit was designed for the quantitative measurement of Sheep IFNG protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 5 pg/mL-200 pg/mL and the sensitivity is 2.5 pg/mL.

  • 別名：
    IFNG ELISA Kit; Interferon gamma ELISA Kit; IFN-gamma ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Ovis aries (Sheep)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    5 pg/mL-200 pg/mL
  • 靈敏度：
    2.5 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of sheep IFN-γ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 98  
    Range % 95-102  
    1:2 Average % 87  
    Range % 84-90  
    1:4 Average % 85  
    Range % 82-89  
    1:8 Average % 93  
    Range % 90-96  
  • 回收率：
    The recovery of sheep IFN-γ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 92 87-95  
    EDTA plasma (n=4) 86 83-89  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    200 2.776 2.798 2.787    
    100 2.088 2.098 2.093    
    50 1.164 1.187 1.176    
    25 0.675 0.633 0.654    
    10 0.264 0.287 0.276    
    5 0.125 0.127 0.126    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation. Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation. Upon IFNG binding, IFNGR1 intracellular domain opens out to allow association of downstream signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation, nuclear translocation and transcription of IFNG-regulated genes. Many of the induced genes are transcription factors such as IRF1 that are able to further drive regulation of a next wave of transcription. Plays a role in class I antigen presentation pathway by inducing a replacement of catalytic proteasome subunits with immunoproteasome subunits. In turn, increases the quantity, quality, and repertoire of peptides for class I MHC loading. Increases the efficiency of peptide generation also by inducing the expression of activator PA28 that associates with the proteasome and alters its proteolytic cleavage preference. Up-regulates as well MHC II complexes on the cell surface by promoting expression of several key molecules such as cathepsins B/CTSB, H/CTSH, and L/CTSL. Participates in the regulation of hematopoietic stem cells during development and under homeostatic conditions by affecting their development, quiescence, and differentiation.
  • 基因功能參考文獻(xiàn)：
    1. gammadelta T cells traveling in the skin-draining afferent lymph are effectors that produce IFN-gamma or IL-17 and express high levels of the skin- and inflammation-seeking molecule E-selectin ligand PMID: 23838472
    2. structural and functional characterization and role in haemonchiasis resistance PMID: 21419496
    3. results suggest that intron 1 of the interferon gamma gene has an important role in resistance to nematode infection in the Texel breed PMID: 16054889
    4. Five unique polymorphic patterns were detected and sequence analysis revealed that these patterns represent five different IFNG alleles. PMID: 16820277
  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Type II (or gamma) interferon family
  • 組織特異性：
    Released primarily from activated T lymphocytes.
  • 數(shù)據(jù)庫鏈接：

    KEGG: oas:443396

    UniGene: Oar.457