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Rat dopamine,DA ELISA Kit

  • 中文名稱：
    大鼠多巴胺(DA)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E08660r
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3900/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
        DA (多巴胺，dopamine) 是一種神經(jīng)遞質(zhì)，在中樞神經(jīng)系統(tǒng)中發(fā)揮重要作用，涉及許多功能，如行為控制、情感調(diào)節(jié)、內(nèi)分泌調(diào)節(jié)和運動協(xié)調(diào)等。
        華美生物所提供的Rat dopamine,DA ELISA Kit屬于ELISA檢測試劑盒，采用雙抗夾心法定量檢測大鼠血清、血漿、組織勻漿樣本中的DA，其靈敏度為0.039 ng/mL，檢測范圍為0.156 ng/mL-10 ng/mL。
     
  • 別名：
    N/A
  • 縮寫：
    DA
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    0.156 ng/mL-10 ng/mL
  • 靈敏度：
    0.039 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Neuroscience
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat DA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:5 Average % 86  
    Range % 84-96  
    1:10 Average % 95  
    Range % 91-100  
    1:20 Average % 84  
    Range % 81-88  
    1:40 Average % 92  
    Range % 88-96  
  • 回收率：
    The recovery of rat DA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 91 87-95  
    EDTA plasma (n=4) 96 94-98  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    10 2.174 2.280 2.227 2.076  
    5 1.784 1.789 1.787 1.636  
    2.5 1.250 1.276 1.263 1.112  
    1.25 0.841 0.875 0.858 0.707  
    0.625 0.435 0.465 0.450 0.299  
    0.312 0.287 0.308 0.298 0.147  
    0.156 0.208 0.223 0.216 0.065  
    0 0.152 0.150 0.151 　  
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻

產(chǎn)品評價

問答及客戶評論

■ 客戶評論
  • Sample type: Serum
    Sample species: Rat
    Review: The DA content was evaluated using the corresponding ELISA kit. A similar pattern was observed in the DA striatal content as presented in panel I, where 6-OHDA leveled it off, while the co-administration of 6-OHDA with A-779 depleted it markedly.
    PMID: 29428223
  • Sample type: Cell-free supernatants
    Sample species: Rat
    Review: It was found that 2 hours after the intravenous injection of PC8 a significant increase of melatonin and serotonin (5-HT) was observed in the brain, whereas injection of PITScomposition (PC8+Lip+Xe) decreased the levels of serotonin and dopamine, although there was a considerable rise in the level of melatonin in both cases
    PMID: 30658098
  • Sample type: Supernatants
    Sample species: Rat
    Review: The supernatant was used to measure concentrations of GFAP, DA, TH, MANF, NGF, and CDNF using commercially available ELISA assays following the manufacturer’s instructions. PEx prevents the loss of DA in 6-OHDA challenged rats greater than MIEx (F (5, 29) = 22.03, p< 0.0001). *significant difference between HC and PC. **significant difference between PEx and PC. ***significant difference between PEx and MIEx (P<0.0001).
    PMID: 31100327
  • Sample type: Supernatant of rats’ brains
    Sample species: Rat
    Review: Effects of L-dopa, sitagliptin, or liraglutide alone or combined with L-dopa on striatal dopamine content of rotenone-induced Parkinsonism in rats. L-dopa/carbidopa (50/25 mg/kg, i.p.), sitagliptin (30 mg/kg, p.o.), or liraglutide (50 μg/kg, s.c.) was administered for 16 days alone or in combination to rats treated with 10 daily doses of rotenone (3 mg/kg/day, s.c.)
    PMID: 30673988
  • Sample type: Brain tissue samples
    Sample species: Rat
    Review: Norepinephrine (NE), serotonin (5-HT), and dopamine (DA) levels in the hypothalamus, hippocampus, and prefrontal cortex were measured in brain tissue samples of recommended volume using commercially available enzyme immunoassay kits specifc for each compound as previously described. DA levels (ng/g tissue) in the hippocampus of stress (n=6, outlier number=2) and control ofspring (n=6, outlier number=2). h DA levels (ng/g tissue) in the hypothalamus of stress (n=6, outlier number=2) and control ofspring (n=6, outlier number=2). i DA levels (ng/g tissue) in the prefrontal cortex of stress (n=5, outlier number=3) and control ofspring (n=5, outlier number=3). Control ofspring: ofspring of control group (black); Stress ofspring: ofspring of maternal rats subject to SDS stimulation (grey); 5-HT: serotonin; NE: norepinephrine; DA: dopamine; *p<0.05
    PMID: 30326843
  • Sample type: Supernatant of brain tissues (100 μl)
    Sample species: Rat
    Review: Effect of IDPU treatment on A) Dopamine B) Glutamate C) Calcium D) Nitrite.Values are expressed as mean ± SEM (n = 6). Statistical analysis by one way ANOVA followed by Tukey's post hoc multiple comparison tests. ### P ≤ 0.001, versus sham group, ***P ≤ 0.001 *P ≤ 0.05 indicates versus 6-OHDA (10 μg) lesioned group.
    PMID: 29567422

靶點詳情

  • 最新研究進展：
    DA是多巴胺的簡稱，是一種重要的神經(jīng)遞質(zhì)。近年來，研究人員發(fā)現(xiàn)DA參與了多種生理和病理過程，如運動功能、認知能力、代謝性疾病、精神疾病等。同時，DA與多種神經(jīng)系統(tǒng)疾病的發(fā)病機制密切相關(guān)，如帕金森病、精神分裂癥等。