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Rat Interleukin 12,IL-12/P70 ELISA KIT

  • 中文名稱(chēng):
    大鼠白介素12(IL-12/P70)酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-E07364r
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥3200/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:

    This Rat IL12/P70 ELISA Kit was designed for the quantitative measurement of Rat IL12/P70 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.12 pg/mL-200 pg/mL and the sensitivity is 0.78 pg/mL .

  • 別名:
    N/A
  • 縮寫(xiě):
    IL-12/P70
  • 種屬:
    Rattus norvegicus (Rat)
  • 樣本類(lèi)型:
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍:
    3.12 pg/mL-200 pg/mL
  • 靈敏度:
    0.78 pg/mL
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測(cè)波長(zhǎng):
    450 nm
  • 研究領(lǐng)域:
    Immunology
  • 測(cè)定原理:
    quantitative
  • 測(cè)定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-12/P70 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:1Average %96
    Range %91-106
    1:2Average %94
    Range %91-98
    1:4Average %93
    Range %89-96
    1:8Average %87
    Range %82-96
  • 回收率:
    The recovery of rat IL-12/P70 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9489-98
    EDTA plasma (n=4)10398-107
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    2002.557 2.656 2.607 2.425
    1002.023 2.122 2.073 1.891
    501.448 1.468 1.458 1.276
    250.928 0.917 0.923 0.741
    12.50.578 0.569 0.574 0.392
    6.250.415 0.405 0.410 0.228
    3.120.303 0.308 0.306 0.124
    00.181 0.182 0.182  
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

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