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  4. Rat Insulin,INS ELISA Kit

Rat Insulin,INS ELISA Kit

  • 中文名稱：
    大鼠胰島素(INS)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E05070r
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
        INS（胰島素）是一種由胰腺分泌的多肽激素，其作用是促進(jìn)體細(xì)胞對(duì)葡萄糖的吸收和利用，從而維持血糖平衡。胰島素的合成和分泌受多種內(nèi)外因素的調(diào)控。體內(nèi)胰島素含量與血糖水平密切相關(guān)，高血糖可刺激胰腺分泌胰島素。
        華美生物所提供的Rat Insulin,INS ELISA Kit屬于ELISA檢測(cè)試劑盒，采用雙抗夾心法定量檢測(cè)大鼠血清、血漿、細(xì)胞培養(yǎng)上清樣本中的INS，其靈敏度為3.9 nIU/mL，檢測(cè)范圍為15.6 nIU/mL-1000 nIU/mL。
     
  • 別名：
    Ins1 ELISA Kit; Ins-1 ELISA Kit; Insulin-1 [Cleaved into: Insulin-1 B chain; Insulin-1 A chain] ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, cell culture supernates
  • 檢測(cè)范圍：
    15.6 nIU/mL-1000 nIU/mL
  • 靈敏度：
    3.9 nIU/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Metabolism
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat INS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:100 Average % 90  
    Range % 85-94  
    1:200 Average % 95  
    Range % 91-99  
    1:400 Average % 84  
    Range % 80-89  
    1:800 Average % 89  
    Range % 84-96  
  • 回收率：
    The recovery of rat INS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 90-100  
    EDTA plasma (n=4) 92 88-96  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    nIU/ml OD1 OD2 Average Corrected  
    1000 2.372 2.234 2.303 2.150  
    500 1.859 1.812 1.836 1.683  
    250 1.429 1.365 1.397 1.244  
    125 1.027 1.097 1.062 0.909  
    62.5 0.576 0.589 0.583 0.430  
    31.2 0.341 0.317 0.329 0.176  
    15.6 0.213 0.207 0.210 0.057  
    0 0.155 0.151 0.153 　  
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評(píng)價(jià)

問答及客戶評(píng)論

■ 常見問題解答
Q:

Seeking for ELISA kits of rat INSULIN.I am working on SD Rats and project related to High Fat Diet induced Metabolic complications.

A:
Thanks for your inquiry.
For the mentiond detection targets,we have the following kit:
#CSB-E05070r,Rat Insulin,INS ELISA Kit
And we use kits to test ordinary rats.While there is not big problems to detect SD Rats.Pls take your actual test results as the real results.

靶點(diǎn)詳情

  • 最新研究進(jìn)展：
    胰島素是由胰島β細(xì)胞分泌的多肽激素，在血糖調(diào)節(jié)和能量代謝中起著重要作用。最新研究表明，胰島素除了其已知的代謝作用外，還可能具有其他生物學(xué)功能，例如參與免疫調(diào)節(jié)和細(xì)胞增殖等。此外，研究人員正在探索利用人工智能和機(jī)器學(xué)習(xí)等技術(shù)預(yù)測(cè)胰島素的作用機(jī)制和治療效果。
  • 功能：
    Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
  • 基因功能參考文獻(xiàn)：
    1. In conclusion, TRPV4 differently regulates insulin mRNA expression in INS-1E cells via ERK1/2 and NO-dependent mechanisms. PMID: 28359774
    2. CART is important for maintaining the beta-cell phenotype. PMID: 28237718
    3. Expression of ghrelin and ghrelin receptor and glucose stimulated insulin secretion (GSIS) of pancreatic islets. PMID: 28410130
    4. glucagon plus insulin increases FGF21 transcription by stimulating ATF4 expression PMID: 28188284
    5. Under insulin stimulation, and in spite of the less activated mTOR function in high altitude rats, low altitude rats had higher glycogen content and reduced levels of gluconeogenic enzymes with a more enhanced insulin signaling, mainly due to higher levels of phosphorylated IRS1. PMID: 28084108
    6. Reduced sortilin levels associate with lower VLDL apo B secretion. Sortilin facilitates VLDL-B100 secretion by insulin sensitive McArdle RH7777 cells. PMID: 27495870
    7. Data show that quercetin acutely stimulates insulin release, inhibits cell proliferation and induces apoptosis of INS-1 beta cells. PMID: 27336168
    8. Insulin deficiency increases the expression of IGF-1 and IGF-1R in renal mesangial cells and the kidney of diabetic rats, which contributes to the development of diabetic nephropathy. PMID: 26775660
    9. Data suggest that rosuvastatin has the potential to increase basal insulin secretion and reduce glucose-induced insulin secretion. PMID: 26986474
    10. These results identify a novel role for Jagn1 in regulating proinsulin biosynthesis. PMID: 26882284
    11. Data suggest glucose-induced insulin secretion is first up-regulated (acute infection), then down-regulated (chronic infection) in beta cells infected with S. aureus; Pla2g4a is up-regulated in acute infection; Pla2g6 is up-regulated in chronic infection. PMID: 26632509
    12. These observations support the hypothesis that glucagon acts via adenylate cyclase to decrease hepatic GSH levels and induce insulin resistance. PMID: 25961284
    13. Gamma conglutin and glibenclamide increased Ins-1 gene expression in diabetic rats. PMID: 24894193
    14. Data suggest neural mechanism of allostatic insulin secretion in male rats fed liquid diet as meals; allostasis leads to postprandial insulin secretion by pancreatic islets suggestive of neural/neurosecretory regulation to prevent hyperglycemia. PMID: 25159330
    15. Maximal upregulation of many unfolded protein response genes in response to mutant proinsulin production required IRE1. PMID: 25011481
    16. Data indicate that, gestational Di-(2-ethylhexyl)phthalate (DEHP) exposure predisposes F1 offspring to glucometabolic dysfunction by down-regulating the expression of critical genes involved in the insulin signalling pathway. PMID: 25232145
    17. analysis of organization of the beta-structure of rat insulin reveals key regions essential for the propagation and cytotoxicity of amyloid fibrils PMID: 24569992
    18. Data suggest that responsiveness of pancreatic beta cells to glucose level resulting in secretion of insulin involves Glp1/Glp1r (glucagon-like peptide-1/glucagon-like peptide-1 receptor) signaling and GLP1/Glp1r agonists act as hypoglycemic agents. PMID: 24425760
    19. Data indicate that miR-183 was decreased and Foxo1 was upregulated during conversion of dental pulp cells (DPCs) to insulin-producing cells. PMID: 24440707
    20. miR-25 and miR-92a are found as direct modulators of insulin expression; overexpression of miR-25 or miR-92a reduced insulin expression;inhibition of miR-25 and miR-92a expression promoted insulin expression and ultimately enhanced glucose-induced insulin secretion PMID: 24084692
    21. B-cell translocation gene 2 (BTG2) is a crucial regulator in GLP-1-induced insulin gene expression and insulin secretion via upregulation of pancreatic duodenal homeobox-1 (PDX-1) in pancreatic beta-cells. PMID: 23703573
    22. investigation of role of microtubules/actin cytoskeleton in insulin-stimulated glucose uptake in skeletal muscle: Data suggest sequence of reorganization of microtubules, actin remodeling, translocation of GLUT4, and glucose uptake. PMID: 22996137
    23. Exogenous and endogenous ghrelin counteracts GLP-1 action to stimulate cAMP signaling and insulin secretion in islet beta-cells PMID: 22750144
    24. adiponectin in the serum from CR animals increases NO* signaling by activating the insulin pathway PMID: 22319612
    25. Insulin entry into muscle involves a saturable process in the vascular endothelium PMID: 22002008
    26. results revealed a novel function for brain insulin in CTA and an additional source of local synthesis, the central and/or basolateral nuclei of the amygdala. PMID: 22119580
    27. Per-Arnt-Sim (PAS) domain-containing protein kinase (PASK) has a role in insulin hypersecretion PMID: 22065581
    28. Estrogen-related receptor gamma (ERRgamma) is a novel transcriptional regulator of phosphatidic acid phosphatase, LIPIN1, and inhibits hepatic insulin signaling PMID: 21911493
    29. NAD(P)H oxidase is an important source of superoxide in pancreatic islets and the activity of NAD(P)H oxidase is involved in the control of insulin secretion by palmitate. PMID: 20857410
    30. Insulin increased plasma membrane GLUT4 and GLUT1 content 30 and 40 min after treatment and CD36 content in plasma membrane fraction. PMID: 21773965
    31. Insulin (Ins) release is altered by anesthetics. PMID: 18165568
    32. insulin stimulates cell proliferation and c-Myc expression in colon cancer cell lines HT29 and Caco-2, intestinal non-cancer cell line IEC-6, and primary fetal rat intestinal cell (FRIC) cultures. PMID: 17993259
    33. the stimulation of glucose uptake in response to insulin or metabolic stress is dependent in cardiomyocytes on the presence of intact microtubules. PMID: 18063688
    34. Insulin down-regulates specific activity of ATP-binding cassette transporter A1 for high density lipoprotein biogenesis through its specific phosphorylation PMID: 21402379
    35. C-peptide may contribute to plaque development and restenosis formation in patients with insulin resistance and early type 2 diabetes mellitus. (C-peptide) PMID: 17068290
    36. AS160 phosphotyrosine-binding domain constructs inhibit insulin-stimulated GLUT4 vesicle fusion with the plasma membrane PMID: 21454690
    37. Data support the hypothesis that brain insulin is required to support optimal baroreflex function and that a decrease in brain insulin contributes to the fall in baroreflex gain during pregnancy. PMID: 21149828
    38. beta2-Syntrophin is a Cdk5 substrate that restrains the motility of insulin secretory granules. PMID: 20886068
    39. Intrauterine growth retardation leads to the functional change of insulin secretion in the newborn. PMID: 20225168
    40. This study identified second messenger systems in neuropeptide Y neurons of arcuate nucleus which regulate neuron activity and physiological processes such as the control of food intake and body weight, under the combined control of insulin and leptin. PMID: 19671839
    41. Leptin and insulin induce mutual resistance for nitric oxide synthase III activation in adipocytes PMID: 19728324
    42. induction of c-myc expression suppresses gene transcription PMID: 11799123
    43. Insulin preincubation effects on rat vessel contractile responses: role of the endothelium cycooxygenase and nitric oxide synthase pathways. PMID: 11824479
    44. mechanisms involved in the insulin-induced acceleration of ET1 contractions; role of the sarcoplasmic reticulum and calcium. PMID: 11824480
    45. pancreatic release of insulin inhibited by ghrelin PMID: 11834435
    46. effects of insulin and the degree of muscle loading on the phosphorylation of Ser(2448), a site in the mammalian target of rapamycin (mTOR) phosphorylated by protein kinase B (PKB) in vitro PMID: 11884412
    47. Glucagon-like peptide-1 analogue LY315902: effect on intestinal motility and release of insulin and somatostatin PMID: 12047915
    48. endogenously released insulin plays an important role in potentiating pancreatic secretion stimulated by exogenous cholecystokinin (CCK) and intraduodenal infusion of casein PMID: 12120208
    49. the signalling pathways and the effects of insulin were compared to those of glutamine and leucine in isolated hepatocytes from normal and streptozotocin-diabetic rats PMID: 12153571
    50. Insulin modulates GLUT4 trafficking at multiple steps, including not only the endocytotic and exocytotic rates, but also the two rate coefficients coupling the three intracellular compartments. PMID: 12450403

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  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Insulin family
  • 數(shù)據(jù)庫(kù)鏈接：

    KEGG: rno:24505

    STRING: 10116.ENSRNOP00000016052

    UniGene: Rn.962