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  4. Rat Glutathione Peroxidase (GSH-PX) ELISA kit

Rat Glutathione Peroxidase (GSH-PX) ELISA kit

  • 中文名稱(chēng)：
    大鼠谷胱甘肽過(guò)氧化物酶(GSH-PX)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E12146r
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
        GPX1 (Glutathione Peroxidase 1)是一種重要的抗氧化酶，能夠催化還原型谷胱甘肽(GSH)對(duì)過(guò)氧化氫和其他過(guò)氧化物進(jìn)行還原，從而保護(hù)細(xì)胞免受氧化損傷。GPX1的缺乏或活性下降與多種疾病的發(fā)生和發(fā)展密切相關(guān)，如心血管疾病、炎癥性腸病等。因此，測(cè)定GPX1的含量可以用來(lái)評(píng)估機(jī)體抗氧化能力的水平，以及某些疾病的風(fēng)險(xiǎn)。
        華美生物所提供的Rat Glutathione Peroxidase (GSH-PX) ELISA kit屬于ELISA檢測(cè)試劑盒，采用雙抗夾心法定量檢測(cè)大鼠血清、血漿、組織勻漿樣本中的GPX1，其靈敏度為3.12 mIU/mL，檢測(cè)范圍為12.5 mIU/mL-800 mIU/mL。
     
  • 別名：
    Gpx1Glutathione peroxidase 1 ELISA Kit; GPx-1 ELISA Kit; GSHPx-1 ELISA Kit; EC 1.11.1.9 ELISA Kit; Cellular glutathione peroxidase ELISA Kit
  • 縮寫(xiě)：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類(lèi)型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    12.5 mIU/mL-800 mIU/mL
  • 靈敏度：
    3.12 mIU/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Cancer
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat GSH-PX in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:200 Average % 92  
    Range % 85-100  
    1:400 Average % 97  
    Range % 94-101  
    1:800 Average % 95  
    Range % 90-99  
    1:1600 Average % 93  
    Range % 87-98  
  • 回收率：
    The recovery of rat GSH-PX spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 94 86-100  
    EDTA plasma (n=4) 100 95-105  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mIU/ml OD1 OD2 Average Corrected  
    800 2.251 2.299 2.275 2.119  
    400 1.845 1.834 1.840 1.684  
    200 1.301 1.324 1.313 1.157  
    100 0.892 0.876 0.884 0.728  
    50 0.553 0.590 0.572 0.416  
    25 0.395 0.381 0.388 0.232  
    12.5 0.251 0.234 0.243 0.087  
    0 0.155 0.157 0.156 　  
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 最新研究進(jìn)展：
    GPX1是一種谷胱甘肽過(guò)氧化物酶，可以清除細(xì)胞內(nèi)的過(guò)氧化氫等活性氧，具有抗氧化和細(xì)胞保護(hù)作用。最新研究表明，GPX1在多種疾病的發(fā)生和發(fā)展中發(fā)揮重要作用。例如，一些研究發(fā)現(xiàn)GPX1與心血管疾病、癌癥和糖尿病等疾病的風(fēng)險(xiǎn)存在相關(guān)性。此外，一些研究還發(fā)現(xiàn)GPX1在免疫調(diào)節(jié)、神經(jīng)保護(hù)和干細(xì)胞分化等方面發(fā)揮著重要作用。
  • 功能：
    Protects the hemoglobin in erythrocytes from oxidative breakdown. In platelets, plays a crucial role of glutathione peroxidase in the arachidonic acid metabolism.
  • 基因功能參考文獻(xiàn)：
    1. Knockdown of nNOS or GPx1 was specific and had broad effects on downstream genes, though it only modestly ameliorated traumatic brain injury-induced working memory deficits, despite widespread genomic changes. PMID: 29016640
    2. The glutathione peroxidase activity and p110-PI3K/Thr308-Akt are both modulated in the same way by oxygen. PMID: 24401635
    3. VPO1 plays an important role in senescence of endothelial cells and endothelial dysfunction by induction of oxidative stress and inflammatory reaction in type 2 diabetic rats. PMID: 26142204
    4. glutathione peroxidase 1 immunoreactivity was diffusely cytoplasmic in some of the hepatocytes PMID: 24867493
    5. Dietary Se-enriched A. bisporus protected against increase in ileum permeability and up-regulated GPx-1 and GPx-2 expression, selenoenzymes relevant to mitigating oxidative stress. PMID: 24962481
    6. It represents the pivotal antioxidant enzyme in vascular endothelium and exerts a protective effect against the presence of coronary artery disease PMID: 24389817
    7. data collectively indicate that GDNF is capable of inducing anti-oxidant activities of intra-cellular GPX-1 and that growth-promoting potential of GDNF and anti-oxidant properties of GPX-1 can, in concert, maximize survival of dopaminergic neurons. PMID: 23472740
    8. Upon selenium fluctuation, the modulation of UGA recoding efficiency depends on the nature of the SECIS, with Gpx1 being more sensitive than Gpx4. PMID: 22614831
    9. GPx levels in the cortex and hippocampus were increased in OLETF rats compared with LETO rats, with an inverse correlation between GPx in the hippocampus and learning score. PMID: 22981416
    10. The glutathione peroxidase represents an important part of the antioxidant defense system. PMID: 20524817
    11. expression of glutathione peroxidase 1 and glutathione reductase via GSH did not appear to coordinate with development, tissue localization or under oxidative stress PMID: 12516874
    12. excess amounts of PLOOH are efficiently eliminated through the hydrolysis by PLA2, and the subsequent reduction of FA hydroperoxide by GPx is the critical step for complete detoxification of oxidized phospholipids in the stomach PMID: 12934674
    13. Over-expression gpx1 genes in primary hippocampal or cortical cultures resulted in increased enzymatic activity of the cognate protein. PMID: 14713308
    14. Gxp1 levels was increased indicating that a reduction in oxidative damage protection may be responsible for accelerated telomere shortening over time, resulting in increased cellular senescence, loss of renal function, and death in male rats. PMID: 16189290
    15. Novel sites of Gpx1 expression were observed in colon lymphatic tissue. PMID: 16229841
    16. Our results suggest that impairment of the GPx (including glutathione)-mediated antioxidant system contributed to epileptogenesis in GEPR-9s. PMID: 18226427
    17. docosahexaenoic acid sensitizes breast cancer cells to anthracyclines through loss of glutathione peroxidase (GPx1) response PMID: 18267129
    18. Selenium requirements are higher for glutathione peroxidase-1 mRNA than gpx1 activity in rat testis. PMID: 19234057
    19. red blood cell glutathione peroxidase (Gpx1) activity in old female rats. PMID: 19398061
    20. Whole blood selenoprotein mRNA levels can be used as molecular biomarkers for assessing Selenium requirements. PMID: 19855070

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  • 亞細(xì)胞定位：
    Cytoplasm.
  • 蛋白家族：
    Glutathione peroxidase family
  • 組織特異性：
    Expressed in liver and lung.
  • 數(shù)據(jù)庫(kù)鏈接：