Rat Fibrinogen,Fbg ELISA Kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

This Rat Fbg ELISA Kit was designed for the quantitative measurement of Rat Fbg protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 0.312 μg/mL-20 μg/mL and the sensitivity is 0.156 μg/mL.
別名：

N/A
縮寫：

Fbg
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.312 μg/mL-20 μg/mL
靈敏度：

0.156 μg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Cardiovascular
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat Fbg in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	99
	Range %	91-103
1:200	Average %	90
	Range %	82-94
1:400	Average %	102
	Range %	91-106
1:800	Average %	101
	Range %	91-105

回收率：

The recovery of rat Fbg spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	100	93-104
EDTA plasma (n=4)	94	90-98

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.