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  4. Rat Collagen Type Ⅲ,Col Ⅲ ELISA KIT

Rat Collagen Type Ⅲ,Col Ⅲ ELISA KIT

  • 中文名稱：
    大鼠Ⅲ型膠原(Col Ⅲ)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E07924r
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3900/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：

    This Rat Col Ⅲ ELISA Kit was designed for the quantitative measurement of Rat Col Ⅲ protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL .

  • 別名：
    Collagen Type 3,Col 3,_x000D_ Collagen Type III,Col III
  • 縮寫：
    Col Ⅲ
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    0.156 ng/mL-10 ng/mL
  • 靈敏度：
    0.039 ng/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat Col Ⅲ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 92  
    Range % 88-96  
    1:2 Average % 98  
    Range % 92-104  
    1:4 Average % 95  
    Range % 92-98  
    1:8 Average % 86  
    Range % 80-94  
  • 回收率：
    The recovery of rat Col Ⅲ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 94 90-98  
    EDTA plasma (n=4) 90 85-95  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    10 2.585 2.532 2.559 2.415  
    5 1.795 1.719 1.757 1.613  
    2.5 1.083 1.021 1.052 0.908  
    1.25 0.657 0.705 0.681 0.537  
    0.625 0.418 0.450 0.434 0.290  
    0.312 0.299 0.314 0.307 0.163  
    0.156 0.242 0.229 0.236 0.092  
    0 0.146 0.141 0.144 　  
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

平均分:
5.0分 - 1 個評價

樣品類型：血清

樣品信息：大鼠

稀釋比：1:50

產(chǎn)品評價： RAT collagen type3, elisa試劑盒檢測大鼠血清，復孔的數(shù)據(jù)較穩(wěn)定。試劑盒溶液給量充足。此外，還發(fā)現(xiàn)該試劑盒可以檢測其他嚙齒動物的血清樣本，但OD值偏小。

By 杜老師