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Rat 8-Hydroxy-desoxyguanosine,8-OHdG ELISA Kit

  • 中文名稱:
    大鼠8羥基脫氧鳥苷(8-OHdG)酶聯(lián)免疫試劑盒
  • 貨號:
    CSB-E10526r
  • 規(guī)格:
    96T/48T
  • 價格:
    ¥3900/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
        8-OHdG,也稱為 8-羥基脫氧鳥苷,是體內(nèi)氧化應激的標志物。它是一種生物標志物,可用于評估氧化損傷的水平和識別潛在的健康問題。 8-OHdG 已被發(fā)現(xiàn)在患有某些疾?。ㄈ绨┌Y、糖尿病、心血管疾病和神經(jīng)退行性疾?。┑膫€體中升高。此外,它還與衰老和空氣污染等環(huán)境壓力因素有關(guān)。研究表明,測量 8-OHdG 水平可以幫助診斷疾病狀態(tài)和監(jiān)測治療效果。因此,它是臨床醫(yī)學中評估患者氧化應激的重要工具。
        華美生物所提供的Rat 8-Hydroxy-desoxyguanosine,8-OHdG ELISA Kit屬于ELISA檢測試劑盒,采用雙抗夾心法定量檢測大鼠血清、血漿、細胞培養(yǎng)上清、組織勻漿樣本中的8-OHdG,其靈敏度為華美生物所提供的0.078 ng/mL,檢測范圍為0.312 ng/mL-20 ng/mL。
     
  • 縮寫:
    8-OHdG
  • 種屬:
    Rattus norvegicus (Rat)
  • 樣本類型:
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測范圍:
    0.312 ng/mL-20 ng/mL
  • 靈敏度:
    0.078 ng/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領(lǐng)域:
    Cell Biology
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rat 8-OHdG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 95  
    Range % 88-98  
    1:2 Average % 96  
    Range % 91-102  
    1:4 Average % 90  
    Range % 85-96  
    1:8 Average % 96  
    Range % 88-99  
  • 回收率:
    The recovery of rat 8-OHdG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 102 98-107  
    EDTA plasma (n=4) 96 90-102  
                 
                 
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    20 1.890 1.780 1.835 1.682  
    10 1.198 1.128 1.163 1.010  
    5 0.756 0.726 0.741 0.588  
    2.5 0.525 0.495 0.510 0.357  
    1.25 0.321 0.318 0.320 0.167  
    0.625 0.272 0.262 0.267 0.114  
    0.312 0.213 0.209 0.211 0.058  
    0 0.154 0.152 0.153    
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

引用文獻

產(chǎn)品評價

靶點詳情

  • 最新研究進展:
        8-OHdG是DNA氧化損傷的標志物,最新研究發(fā)現(xiàn),其與肺癌、肝癌、結(jié)直腸癌、卵巢癌等多種癌癥的發(fā)生和發(fā)展有關(guān)。研究人員通過對癌癥患者和對照組進行對比分析,發(fā)現(xiàn)8-OHdG的水平在癌癥患者中明顯升高,因此可以作為腫瘤的生物標志物之一。
        此外,8-OHdG的水平還可以反映環(huán)境暴露和生活方式對人體DNA的影響。最新研究表明,8-OHdG的水平與吸煙、飲酒、肥胖、缺乏鍛煉等不良生活習慣有關(guān)。因此,8-OHdG的測量可以用作評估生活方式和環(huán)境暴露的生物標志物。
        另外,一些研究還表明,8-OHdG可能與一些神經(jīng)退行性疾病的發(fā)生和發(fā)展有關(guān),包括帕金森病和阿爾茨海默病。在這些疾病中,氧化應激和DNA氧化損傷可能是致病機制之一,而8-OHdG的測量可以用作評估疾病進展和治療效果的生物標志物。