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  4. Rat 5-hydroxyindolacetic acid,5-HIAA ELISA Kit

Rat 5-hydroxyindolacetic acid,5-HIAA ELISA Kit

  • 中文名稱(chēng)：
    大鼠5羥基吲哚乙酸(5-HIAA)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E13983r
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：

    This Rat 5-HIAA ELISA Kit was designed for the quantitative measurement of Rat 5-HIAA protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 25 ng/mL-400 ng/mL and the sensitivity is 25 ng/mL.

  • 縮寫(xiě)：
    5-HIAA
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類(lèi)型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    25 ng/mL-400 ng/mL
  • 靈敏度：
    25 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Cancer
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat 5-HIAA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 95  
    Range % 89-100  
    1:2 Average % 93  
    Range % 88-97  
    1:4 Average % 98  
    Range % 94-100  
    1:8 Average % 98  
    Range % 94-102  
  • 回收率：
    The recovery of rat 5-HIAA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 92 87-96  
    EDTA plasma (n=4) 94 91-98  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    400 0.283 0.278 0.281    
    200 0.540 0.527 0.534    
    100 0.834 0.901 0.868    
    50 1.698 1.676 1.687    
    25 2.616 2.524 2.570    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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