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  4. Rat 5'-nucleotidase/cluster of differentiation 73 (NT5E/CD73) ELISA kit

Rat 5'-nucleotidase/cluster of differentiation 73 (NT5E/CD73) ELISA kit

  • 中文名稱：
    大鼠5` -核苷酸酶，胞膜(NT5E/CD73)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E14100r
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 別名：
    Nt5e ELISA kit; Nt5 ELISA kit; Nte5'-nucleotidase ELISA kit; 5'-NT ELISA kit; EC 3.1.3.5 ELISA kit; Ecto-5'-nucleotidase ELISA kit; CD antigen CD73 ELISA kit
  • 縮寫：
    NT5E/CD73
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell culture supernates
  • 檢測范圍：
    0.625 mIU/mL-40 mIU/mL
  • 靈敏度：
    0.156 mIU/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Cardiovascular
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat CD73 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %90
    Range %87-96
    1:2Average %87
    Range %82-95
    1:4Average %90
    Range %85-97
    1:8Average %93
    Range %86-101
  • 回收率：
    The recovery of rat CD73 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9590-101
    EDTA plasma (n=4)9186-98
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mIU/mlOD1OD2AverageCorrected
    402.504 2.416 2.460 2.358
    201.933 1.824 1.879 1.777
    101.386 1.283 1.335 1.233
    50.814 0.749 0.782 0.680
    2.50.424 0.397 0.411 0.309
    1.250.228 0.213 0.221 0.119
    0.6250.154 0.145 0.150 0.048
    00.103 0.101 0.102 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Hydrolyzes extracellular nucleotides into membrane permeable nucleosides.
  • 亞細胞定位：
    Cell membrane; Lipid-anchor, GPI-anchor.
  • 蛋白家族：
    5'-nucleotidase family
  • 數(shù)據(jù)庫鏈接：