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Rabbit stromal cell derived factor 1 (SDF-1/CXCL12) ELISA kit

  • 中文名稱:
    兔基質(zhì)細(xì)胞衍生因子-1(SDF-1/CXCL12)酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-E12656Rb
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥3800/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:

    This Rabbit SDF-1/CXCL12 ELISA Kit was designed for the quantitative measurement of Rabbit SDF-1/CXCL12 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.57 ng/mL-10 ng/mL and the sensitivity is 0.36 pg/mL.

  • 縮寫:
    SDF-1/CXCL12
  • 種屬:
    Oryctolagus cuniculus (Rabbit)
  • 樣本類型:
    serum, plasma, tissue homogenates.
  • 檢測(cè)范圍:
    0.625 ng/ml-10 ng/ml.
  • 靈敏度:
    0.125 ng/ml.
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測(cè)波長(zhǎng):
    450 nm
  • 研究領(lǐng)域:
    Immunology
  • 測(cè)定原理:
    quantitative
  • 測(cè)定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rabbit SDF-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 102  
    Range % 88-106  
    1:2 Average % 95  
    Range % 89-102  
    1:4 Average % 88  
    Range % 85-94  
    1:8 Average % 99  
    Range % 93-104  
  • 回收率:
    The recovery of rabbit SDF-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 104 100-108  
    EDTA plasma (n=4) 93 91-97  
                 
                 
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    10 2.167 1.999 2.083 2.046  
    5.71 1.154 1.074 1.114 1.077  
    2.28 0.399 0.384 0.392 0.355  
    1.14 0.211 0.212 0.212 0.175  
    0.57 0.114 0.111 0.113 0.076  
    0 0.035 0.038 0.037    
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

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