This Rabbit CTX-Ⅱ ELISA Kit was designed for the quantitative measurement of Rabbit CTX-Ⅱ protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL.
別名:
cross linked C-telopeptide of type 2 collagen (CTX-2),_x000D_
cross linked C-telopeptide of type II collagen (CTX-II)
縮寫:
CTX-Ⅱ
種屬:
Oryctolagus cuniculus (Rabbit)
樣本類型:
serum, plasma, tissue homogenates
檢測(cè)范圍:
0.625 ng/mL-40 ng/mL
靈敏度:
0.156 ng/mL
反應(yīng)時(shí)間:
1-5h
樣本體積:
50-100ul
檢測(cè)波長(zhǎng):
450 nm
研究領(lǐng)域:
Others
測(cè)定原理:
quantitative
測(cè)定方法:
Sandwich
精密度:
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of rabbit CTX-Ⅱ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample
Serum(n=4)
1:1
Average %
95
Range %
89-103
1:2
Average %
90
Range %
86-99
1:4
Average %
95
Range %
90-102
1:8
Average %
92
Range %
83-99
回收率:
The recovery of rabbit CTX-Ⅱ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type
Average % Recovery
Range
Serum (n=5)
97
92-103
EDTA plasma (n=4)
95
89-106
標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.